Efficient hydrolysis of cottonseed oil with Amano lipase entrapped on hydrolysed polyvinyl alcohol gel pellets optimized via factorial design

• Published in: Current Organic Chemistry
• Main Authors: Talpur, Farah N., Khan, Sidra, Unar, Ahsanullah, Afridi, Hassan Imran, Ibrahim, Adnan, Bashir, Muhammad Sohail
• Format: Article in Journal/Newspaper
• Language: English
• Published: Bentham Science Publishers Ltd. 2023
• Subjects: Organic Chemistry; Antarc*; Antarctica
• Online Access: http://dx.doi.org/10.2174/1385272827666230912164358

Abstract: The aim of this study was to obtain the optimum hydrolysis of cottonseed oil via immobilized lipases (Lipases Rhizopus oryzae, SL from Pseudomonas (Burkholderia) cepacia, Amano lipase from mucor javanica, ANL from Aspergillus niger lipase, TL from Pseudomonas stutzeri, QLM from Alcaligenes sp., Triacylglycerol lipase (EC 3.1.1.3), PL from Alcaligenes sp, AL from lysosomal acid lipase, Candida Antarctica (CA), and Candida cylindrea (CC) on hydrolysed polyvinyl alcohol (PVA) gel pellets. The production of free fatty acids by the hydrolysis of triglycerides from several sources is an important component in the economic exploitation of naturally produced renewable raw materials. Objective: This study aims to obtain the optimum hydrolysis of cottonseed oil via immobilized lipases on hydrolysed Polyvinyl Alcohol (PVA) gel pellets. Various lipases, including Rhizopus oryzea, SL, Amano lipase from mucor javanica, ANL, TL, QLM, EC, PL, AL, CA, and CC were screened for optimum hydrolysis of cottonseed oil. Following the selection of lipase for hydrolysis of cottonseed oil, transparent PVA gel was synthesized by physical gelation method employing Dimethyl sulfoxide and a water system. In the last step, immobilization of selected lipase i.e. Amano lipase for cotton seed oil was carried out at 4 ᵒC for one hour. After successful synthesis and immobilization, the PVA gel was characterized by FTIR and SEM analysis. Finally, factorial design was employed for optimization of experimental variables such as pH, time and amount of oil for both oils hydrolysis by lipase immobilized PVA. The obtained results indicated that lipase Amano was found better with higher free fatty acids (FFA) yields (with 98.38%) in 5 hours using 3g of cottonseed oil, suggesting that PVA immobilized Amano lipase is an efficient catalyst to hydrolyze the cottonseed oil. The overall study indicated that hydrolysed PVA gel is an effective biocompatible solid support for immobilization of lipases to obtain higher FFA from cottonseed oil following ...