Participation of CWINV and SUS Genes in Sucrose Utilization in the Disruption of Cambium Derivatives Differentiation of Silver Birch
Background: The mechanisms that control the accumulation of woody biomass are of great interest to the study. Invertase and sucrose synthase are enzymes that are vital for distributing carbon in various biosynthetic pathways. Karelian birch (Betula pendula var. carelica) is a form of silver birch (B...
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Online Access: | http://dx.doi.org/10.2174/0109298665309207240621094227 |
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crbenthamsciepub:10.2174/0109298665309207240621094227 2024-09-15T18:16:20+00:00 Participation of CWINV and SUS Genes in Sucrose Utilization in the Disruption of Cambium Derivatives Differentiation of Silver Birch Moshchenskaya, Yulia Leonidovna Galibina, Natalia Alekseevna Serkova, Aleksandra Aleksandrovna Tarelkina, Tatyana Vladimirovna Nikerova, Ksenia Michailovna Korzhenevsky, Maksim Anatol’evich Sofronova, Irina Nikolaevna Semenova, Ludmila Igorevna 2024 http://dx.doi.org/10.2174/0109298665309207240621094227 en eng Bentham Science Publishers Ltd. Protein & Peptide Letters volume 31 ISSN 0929-8665 journal-article 2024 crbenthamsciepub https://doi.org/10.2174/0109298665309207240621094227 2024-07-11T04:11:45Z Background: The mechanisms that control the accumulation of woody biomass are of great interest to the study. Invertase and sucrose synthase are enzymes that are vital for distributing carbon in various biosynthetic pathways. Karelian birch (Betula pendula var. carelica) is a form of silver birch (B. pendula Roth) and is characterized by disruption of the differentiation of cambium derivatives towards both the xylem and phloem, which leads to a change in the proportion of the conducting tissues' structural elements and the figured wood formation. We researched the expression profiles of genes encoding sucrose-cleaving enzymes (CWINV and SUS gene families) and genes encoding CVIF protein, which is responsible for the post-translational regulation of the cell wall invertase activity Object: In our study, 16-year-old common silver birch (Betula pendula var. pendula) and Karelian birch were used for sampling non-figured and figured trunk section tissues, respectively. Samples were selected for the research based on the radial vector: non-conductive, conductive phloem, cambial zone - differentiating xylem - mature xylem. Method: The enzyme's activity was investigated by biochemical methods. RT-PCR method was used to determine the level of gene expression. Anatomical and morphological methods were used to determine the stage of differentiation of xylem cambial derivatives. Results: Our research revealed a shift in the composition of xylem components in figured Karelian birch, characterized by increased parenchymatization and reduced vessel quantity. In all studied trunk tissues of Karelian birch, compared with common silver birch, an increase in the expression of the CWINV gene family and the SUS3 gene and a decrease in the expression of SUS4 were shown. Conclusion: Therefore, the increase in parenchymatization in figured Karelian birch is linked to a shift in sucrose metabolism towards the apoplastic pathway, indicated by a higher cell wall invertase activity and gene expression. The expression of the SUS4 gene ... Article in Journal/Newspaper karelian Bentham Science Publishers Protein & Peptide Letters 31 6 479 489 |
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English |
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Background: The mechanisms that control the accumulation of woody biomass are of great interest to the study. Invertase and sucrose synthase are enzymes that are vital for distributing carbon in various biosynthetic pathways. Karelian birch (Betula pendula var. carelica) is a form of silver birch (B. pendula Roth) and is characterized by disruption of the differentiation of cambium derivatives towards both the xylem and phloem, which leads to a change in the proportion of the conducting tissues' structural elements and the figured wood formation. We researched the expression profiles of genes encoding sucrose-cleaving enzymes (CWINV and SUS gene families) and genes encoding CVIF protein, which is responsible for the post-translational regulation of the cell wall invertase activity Object: In our study, 16-year-old common silver birch (Betula pendula var. pendula) and Karelian birch were used for sampling non-figured and figured trunk section tissues, respectively. Samples were selected for the research based on the radial vector: non-conductive, conductive phloem, cambial zone - differentiating xylem - mature xylem. Method: The enzyme's activity was investigated by biochemical methods. RT-PCR method was used to determine the level of gene expression. Anatomical and morphological methods were used to determine the stage of differentiation of xylem cambial derivatives. Results: Our research revealed a shift in the composition of xylem components in figured Karelian birch, characterized by increased parenchymatization and reduced vessel quantity. In all studied trunk tissues of Karelian birch, compared with common silver birch, an increase in the expression of the CWINV gene family and the SUS3 gene and a decrease in the expression of SUS4 were shown. Conclusion: Therefore, the increase in parenchymatization in figured Karelian birch is linked to a shift in sucrose metabolism towards the apoplastic pathway, indicated by a higher cell wall invertase activity and gene expression. The expression of the SUS4 gene ... |
format |
Article in Journal/Newspaper |
author |
Moshchenskaya, Yulia Leonidovna Galibina, Natalia Alekseevna Serkova, Aleksandra Aleksandrovna Tarelkina, Tatyana Vladimirovna Nikerova, Ksenia Michailovna Korzhenevsky, Maksim Anatol’evich Sofronova, Irina Nikolaevna Semenova, Ludmila Igorevna |
spellingShingle |
Moshchenskaya, Yulia Leonidovna Galibina, Natalia Alekseevna Serkova, Aleksandra Aleksandrovna Tarelkina, Tatyana Vladimirovna Nikerova, Ksenia Michailovna Korzhenevsky, Maksim Anatol’evich Sofronova, Irina Nikolaevna Semenova, Ludmila Igorevna Participation of CWINV and SUS Genes in Sucrose Utilization in the Disruption of Cambium Derivatives Differentiation of Silver Birch |
author_facet |
Moshchenskaya, Yulia Leonidovna Galibina, Natalia Alekseevna Serkova, Aleksandra Aleksandrovna Tarelkina, Tatyana Vladimirovna Nikerova, Ksenia Michailovna Korzhenevsky, Maksim Anatol’evich Sofronova, Irina Nikolaevna Semenova, Ludmila Igorevna |
author_sort |
Moshchenskaya, Yulia Leonidovna |
title |
Participation of CWINV and SUS Genes in Sucrose Utilization in the Disruption of Cambium Derivatives Differentiation of Silver Birch |
title_short |
Participation of CWINV and SUS Genes in Sucrose Utilization in the Disruption of Cambium Derivatives Differentiation of Silver Birch |
title_full |
Participation of CWINV and SUS Genes in Sucrose Utilization in the Disruption of Cambium Derivatives Differentiation of Silver Birch |
title_fullStr |
Participation of CWINV and SUS Genes in Sucrose Utilization in the Disruption of Cambium Derivatives Differentiation of Silver Birch |
title_full_unstemmed |
Participation of CWINV and SUS Genes in Sucrose Utilization in the Disruption of Cambium Derivatives Differentiation of Silver Birch |
title_sort |
participation of cwinv and sus genes in sucrose utilization in the disruption of cambium derivatives differentiation of silver birch |
publisher |
Bentham Science Publishers Ltd. |
publishDate |
2024 |
url |
http://dx.doi.org/10.2174/0109298665309207240621094227 |
genre |
karelian |
genre_facet |
karelian |
op_source |
Protein & Peptide Letters volume 31 ISSN 0929-8665 |
op_doi |
https://doi.org/10.2174/0109298665309207240621094227 |
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Protein & Peptide Letters |
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31 |
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6 |
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479 |
op_container_end_page |
489 |
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1810454344900804608 |