mRNA Stability and Polysome Loss in Hibernating Arctic Ground Squirrels ( Spermophilus parryii )
ABSTRACT All small mammalian hibernators periodically rewarm from torpor to high, euthermic body temperatures for brief intervals throughout the hibernating season. The functional significance of these arousal episodes is unknown, but one suggestion is that rewarming may be related to replacement of...
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crasmicro:10.1128/mcb.20.17.6374-6379.2000 2023-05-15T14:57:59+02:00 mRNA Stability and Polysome Loss in Hibernating Arctic Ground Squirrels ( Spermophilus parryii ) Knight, Jason E. Narus, Erin Nicol Martin, Sandra L. Jacobson, Allan Barnes, Brian M. Boyer, Bert B. 2000 http://dx.doi.org/10.1128/mcb.20.17.6374-6379.2000 https://journals.asm.org/doi/pdf/10.1128/MCB.20.17.6374-6379.2000 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Molecular and Cellular Biology volume 20, issue 17, page 6374-6379 ISSN 0270-7306 1098-5549 Cell Biology Molecular Biology journal-article 2000 crasmicro https://doi.org/10.1128/mcb.20.17.6374-6379.2000 2022-10-30T09:13:12Z ABSTRACT All small mammalian hibernators periodically rewarm from torpor to high, euthermic body temperatures for brief intervals throughout the hibernating season. The functional significance of these arousal episodes is unknown, but one suggestion is that rewarming may be related to replacement of gene products lost during torpor due to degradation of mRNA. To assess the stability of mRNA as a function of the hibernation state, we examined the poly(A) tail lengths of liver mRNA from arctic ground squirrels sacrificed during four hibernation states (early and late during a torpor bout and early and late following arousal from torpor) and from active ground squirrels sacrificed in the summer. Poly(A) tail lengths were not altered during torpor, suggesting either that mRNA is stabilized or that transcription continues during torpor. In mRNA isolated from torpid ground squirrels, we observed a pattern of 12 poly(A) residues at greater densities approximately every 27 nucleotides along the poly(A) tail, which is a pattern consistent with binding of poly(A)-binding protein. The intensity of this pattern was significantly reduced following arousal from torpor and undetectable in mRNA obtained from summer ground squirrels. Analyses of polysome profiles revealed a significant reduction in polyribosomes in torpid animals, indicating that translation is depressed during torpor. Article in Journal/Newspaper Arctic ASM Journals (American Society for Microbiology - via Crossref) Arctic Molecular and Cellular Biology 20 17 6374 6379 |
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ASM Journals (American Society for Microbiology - via Crossref) |
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language |
English |
topic |
Cell Biology Molecular Biology |
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Cell Biology Molecular Biology Knight, Jason E. Narus, Erin Nicol Martin, Sandra L. Jacobson, Allan Barnes, Brian M. Boyer, Bert B. mRNA Stability and Polysome Loss in Hibernating Arctic Ground Squirrels ( Spermophilus parryii ) |
topic_facet |
Cell Biology Molecular Biology |
description |
ABSTRACT All small mammalian hibernators periodically rewarm from torpor to high, euthermic body temperatures for brief intervals throughout the hibernating season. The functional significance of these arousal episodes is unknown, but one suggestion is that rewarming may be related to replacement of gene products lost during torpor due to degradation of mRNA. To assess the stability of mRNA as a function of the hibernation state, we examined the poly(A) tail lengths of liver mRNA from arctic ground squirrels sacrificed during four hibernation states (early and late during a torpor bout and early and late following arousal from torpor) and from active ground squirrels sacrificed in the summer. Poly(A) tail lengths were not altered during torpor, suggesting either that mRNA is stabilized or that transcription continues during torpor. In mRNA isolated from torpid ground squirrels, we observed a pattern of 12 poly(A) residues at greater densities approximately every 27 nucleotides along the poly(A) tail, which is a pattern consistent with binding of poly(A)-binding protein. The intensity of this pattern was significantly reduced following arousal from torpor and undetectable in mRNA obtained from summer ground squirrels. Analyses of polysome profiles revealed a significant reduction in polyribosomes in torpid animals, indicating that translation is depressed during torpor. |
format |
Article in Journal/Newspaper |
author |
Knight, Jason E. Narus, Erin Nicol Martin, Sandra L. Jacobson, Allan Barnes, Brian M. Boyer, Bert B. |
author_facet |
Knight, Jason E. Narus, Erin Nicol Martin, Sandra L. Jacobson, Allan Barnes, Brian M. Boyer, Bert B. |
author_sort |
Knight, Jason E. |
title |
mRNA Stability and Polysome Loss in Hibernating Arctic Ground Squirrels ( Spermophilus parryii ) |
title_short |
mRNA Stability and Polysome Loss in Hibernating Arctic Ground Squirrels ( Spermophilus parryii ) |
title_full |
mRNA Stability and Polysome Loss in Hibernating Arctic Ground Squirrels ( Spermophilus parryii ) |
title_fullStr |
mRNA Stability and Polysome Loss in Hibernating Arctic Ground Squirrels ( Spermophilus parryii ) |
title_full_unstemmed |
mRNA Stability and Polysome Loss in Hibernating Arctic Ground Squirrels ( Spermophilus parryii ) |
title_sort |
mrna stability and polysome loss in hibernating arctic ground squirrels ( spermophilus parryii ) |
publisher |
American Society for Microbiology |
publishDate |
2000 |
url |
http://dx.doi.org/10.1128/mcb.20.17.6374-6379.2000 https://journals.asm.org/doi/pdf/10.1128/MCB.20.17.6374-6379.2000 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Molecular and Cellular Biology volume 20, issue 17, page 6374-6379 ISSN 0270-7306 1098-5549 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/mcb.20.17.6374-6379.2000 |
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Molecular and Cellular Biology |
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20 |
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17 |
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6374 |
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6379 |
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1766330067970949120 |