Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen Vibrio shilonii AK1
ABSTRACT Recent application of mutation accumulation techniques combined with whole-genome sequencing (MA/WGS) has greatly promoted studies of spontaneous mutation. However, such explorations have rarely been conducted on marine organisms, and it is unclear how marine habitats have influenced genome...
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crasmicro:10.1128/mbio.01021-17 2024-04-07T07:55:09+00:00 Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen Vibrio shilonii AK1 Strauss, Chloe Long, Hongan Patterson, Caitlyn E. Te, Ronald Lynch, Michael Moran, Nancy A. Army Research Office HHS | National Institutes of Health 2017 http://dx.doi.org/10.1128/mbio.01021-17 https://journals.asm.org/doi/pdf/10.1128/mBio.01021-17 en eng American Society for Microbiology https://creativecommons.org/licenses/by/4.0/ https://journals.asm.org/non-commercial-tdm-license mBio volume 8, issue 4 ISSN 2161-2129 2150-7511 Virology Microbiology journal-article 2017 crasmicro https://doi.org/10.1128/mbio.01021-17 2024-03-08T00:24:45Z ABSTRACT Recent application of mutation accumulation techniques combined with whole-genome sequencing (MA/WGS) has greatly promoted studies of spontaneous mutation. However, such explorations have rarely been conducted on marine organisms, and it is unclear how marine habitats have influenced genome stability. This report resolves the mutation rate and spectrum of the coral reef pathogen Vibrio shilonii , which causes coral bleaching and endangers the biodiversity maintained by coral reefs. We found that its mutation rate and spectrum are highly similar to those of other studied bacteria from various habitats, despite the saline environment. The mutational properties of this marine bacterium are thus controlled by other general evolutionary forces such as natural selection and genetic drift. We also found that as pH drops, the mutation rate decreases and the mutation spectrum is biased in the direction of generating G/C nucleotides. This implies that evolutionary features of this organism and perhaps other marine microbes might be altered by the increasingly acidic ocean water caused by excess CO 2 emission. Nonetheless, further exploration is needed as the pH range tested in this study was rather narrow and many other possible mutation determinants, such as carbonate increase, are associated with ocean acidification. IMPORTANCE This study explored the pH dependence of a bacterial genome-wide mutation rate. We discovered that the genome-wide rates of appearance of most mutation types decrease linearly and that the mutation spectrum is biased in generating more G/C nucleotides with pH drop in the coral reef pathogen V. shilonii . Article in Journal/Newspaper Ocean acidification ASM Journals (American Society for Microbiology) mBio 8 4 |
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English |
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Virology Microbiology |
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Virology Microbiology Strauss, Chloe Long, Hongan Patterson, Caitlyn E. Te, Ronald Lynch, Michael Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen Vibrio shilonii AK1 |
topic_facet |
Virology Microbiology |
description |
ABSTRACT Recent application of mutation accumulation techniques combined with whole-genome sequencing (MA/WGS) has greatly promoted studies of spontaneous mutation. However, such explorations have rarely been conducted on marine organisms, and it is unclear how marine habitats have influenced genome stability. This report resolves the mutation rate and spectrum of the coral reef pathogen Vibrio shilonii , which causes coral bleaching and endangers the biodiversity maintained by coral reefs. We found that its mutation rate and spectrum are highly similar to those of other studied bacteria from various habitats, despite the saline environment. The mutational properties of this marine bacterium are thus controlled by other general evolutionary forces such as natural selection and genetic drift. We also found that as pH drops, the mutation rate decreases and the mutation spectrum is biased in the direction of generating G/C nucleotides. This implies that evolutionary features of this organism and perhaps other marine microbes might be altered by the increasingly acidic ocean water caused by excess CO 2 emission. Nonetheless, further exploration is needed as the pH range tested in this study was rather narrow and many other possible mutation determinants, such as carbonate increase, are associated with ocean acidification. IMPORTANCE This study explored the pH dependence of a bacterial genome-wide mutation rate. We discovered that the genome-wide rates of appearance of most mutation types decrease linearly and that the mutation spectrum is biased in generating more G/C nucleotides with pH drop in the coral reef pathogen V. shilonii . |
author2 |
Moran, Nancy A. Army Research Office HHS | National Institutes of Health |
format |
Article in Journal/Newspaper |
author |
Strauss, Chloe Long, Hongan Patterson, Caitlyn E. Te, Ronald Lynch, Michael |
author_facet |
Strauss, Chloe Long, Hongan Patterson, Caitlyn E. Te, Ronald Lynch, Michael |
author_sort |
Strauss, Chloe |
title |
Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen Vibrio shilonii AK1 |
title_short |
Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen Vibrio shilonii AK1 |
title_full |
Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen Vibrio shilonii AK1 |
title_fullStr |
Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen Vibrio shilonii AK1 |
title_full_unstemmed |
Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen Vibrio shilonii AK1 |
title_sort |
genome-wide mutation rate response to ph change in the coral reef pathogen vibrio shilonii ak1 |
publisher |
American Society for Microbiology |
publishDate |
2017 |
url |
http://dx.doi.org/10.1128/mbio.01021-17 https://journals.asm.org/doi/pdf/10.1128/mBio.01021-17 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
mBio volume 8, issue 4 ISSN 2161-2129 2150-7511 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/mbio.01021-17 |
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mBio |
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8 |
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4 |
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1795672130450358272 |