Nonribosomal Peptides, Key Biocontrol Components for Pseudomonas fluorescens In5, Isolated from a Greenlandic Suppressive Soil
ABSTRACT Potatoes are cultivated in southwest Greenland without the use of pesticides and with limited crop rotation. Despite the fact that plant-pathogenic fungi are present, no severe-disease outbreaks have yet been observed. In this report, we document that a potato soil at Inneruulalik in southe...
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crasmicro:10.1128/mbio.00079-15 2024-09-15T18:09:35+00:00 Nonribosomal Peptides, Key Biocontrol Components for Pseudomonas fluorescens In5, Isolated from a Greenlandic Suppressive Soil Michelsen, Charlotte F. Watrous, Jeramie Glaring, Mikkel A. Kersten, Roland Koyama, Nobuhiro Dorrestein, Pieter C. Stougaard, Peter Greenberg, E. Peter 2015 http://dx.doi.org/10.1128/mbio.00079-15 https://journals.asm.org/doi/pdf/10.1128/mBio.00079-15 en eng American Society for Microbiology http://creativecommons.org/licenses/by-nc-sa/3.0/ https://journals.asm.org/non-commercial-tdm-license mBio volume 6, issue 2 ISSN 2161-2129 2150-7511 journal-article 2015 crasmicro https://doi.org/10.1128/mbio.00079-15 2024-08-26T04:06:00Z ABSTRACT Potatoes are cultivated in southwest Greenland without the use of pesticides and with limited crop rotation. Despite the fact that plant-pathogenic fungi are present, no severe-disease outbreaks have yet been observed. In this report, we document that a potato soil at Inneruulalik in southern Greenland is suppressive against Rhizoctonia solani Ag3 and uncover the suppressive antifungal mechanism of a highly potent biocontrol bacterium, Pseudomonas fluorescens In5, isolated from the suppressive potato soil. A combination of molecular genetics, genomics, and matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) imaging mass spectrometry (IMS) revealed an antifungal genomic island in P. fluorescens In5 encoding two nonribosomal peptides, nunamycin and nunapeptin, which are key components for the biocontrol activity by strain In5 in vitro and in soil microcosm experiments. Furthermore, complex microbial behaviors were highlighted. Whereas nunamycin was demonstrated to inhibit the mycelial growth of R. solani Ag3, but not that of Pythium aphanidermatum , nunapeptin instead inhibited P. aphanidermatum but not R. solani Ag3. Moreover, the synthesis of nunamycin by P. fluorescens In5 was inhibited in the presence of P. aphanidermatum. Further characterization of the two peptides revealed nunamycin to be a monochlorinated 9-amino-acid cyclic lipopeptide with similarity to members of the syringomycin group, whereas nunapeptin was a 22-amino-acid cyclic lipopeptide with similarity to corpeptin and syringopeptin. IMPORTANCE Crop rotation and systematic pest management are used to only a limited extent in Greenlandic potato farming. Nonetheless, although plant-pathogenic fungi are present in the soil, the farmers do not experience major plant disease outbreaks. Here, we show that a Greenlandic potato soil is suppressive against Rhizoctonia solani , and we unravel the key biocontrol components for Pseudomonas fluorescens In5, one of the potent biocontrol bacteria isolated from this Greenlandic ... Article in Journal/Newspaper Greenland greenlandic ASM Journals (American Society for Microbiology) mBio 6 2 |
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English |
description |
ABSTRACT Potatoes are cultivated in southwest Greenland without the use of pesticides and with limited crop rotation. Despite the fact that plant-pathogenic fungi are present, no severe-disease outbreaks have yet been observed. In this report, we document that a potato soil at Inneruulalik in southern Greenland is suppressive against Rhizoctonia solani Ag3 and uncover the suppressive antifungal mechanism of a highly potent biocontrol bacterium, Pseudomonas fluorescens In5, isolated from the suppressive potato soil. A combination of molecular genetics, genomics, and matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) imaging mass spectrometry (IMS) revealed an antifungal genomic island in P. fluorescens In5 encoding two nonribosomal peptides, nunamycin and nunapeptin, which are key components for the biocontrol activity by strain In5 in vitro and in soil microcosm experiments. Furthermore, complex microbial behaviors were highlighted. Whereas nunamycin was demonstrated to inhibit the mycelial growth of R. solani Ag3, but not that of Pythium aphanidermatum , nunapeptin instead inhibited P. aphanidermatum but not R. solani Ag3. Moreover, the synthesis of nunamycin by P. fluorescens In5 was inhibited in the presence of P. aphanidermatum. Further characterization of the two peptides revealed nunamycin to be a monochlorinated 9-amino-acid cyclic lipopeptide with similarity to members of the syringomycin group, whereas nunapeptin was a 22-amino-acid cyclic lipopeptide with similarity to corpeptin and syringopeptin. IMPORTANCE Crop rotation and systematic pest management are used to only a limited extent in Greenlandic potato farming. Nonetheless, although plant-pathogenic fungi are present in the soil, the farmers do not experience major plant disease outbreaks. Here, we show that a Greenlandic potato soil is suppressive against Rhizoctonia solani , and we unravel the key biocontrol components for Pseudomonas fluorescens In5, one of the potent biocontrol bacteria isolated from this Greenlandic ... |
author2 |
Greenberg, E. Peter |
format |
Article in Journal/Newspaper |
author |
Michelsen, Charlotte F. Watrous, Jeramie Glaring, Mikkel A. Kersten, Roland Koyama, Nobuhiro Dorrestein, Pieter C. Stougaard, Peter |
spellingShingle |
Michelsen, Charlotte F. Watrous, Jeramie Glaring, Mikkel A. Kersten, Roland Koyama, Nobuhiro Dorrestein, Pieter C. Stougaard, Peter Nonribosomal Peptides, Key Biocontrol Components for Pseudomonas fluorescens In5, Isolated from a Greenlandic Suppressive Soil |
author_facet |
Michelsen, Charlotte F. Watrous, Jeramie Glaring, Mikkel A. Kersten, Roland Koyama, Nobuhiro Dorrestein, Pieter C. Stougaard, Peter |
author_sort |
Michelsen, Charlotte F. |
title |
Nonribosomal Peptides, Key Biocontrol Components for Pseudomonas fluorescens In5, Isolated from a Greenlandic Suppressive Soil |
title_short |
Nonribosomal Peptides, Key Biocontrol Components for Pseudomonas fluorescens In5, Isolated from a Greenlandic Suppressive Soil |
title_full |
Nonribosomal Peptides, Key Biocontrol Components for Pseudomonas fluorescens In5, Isolated from a Greenlandic Suppressive Soil |
title_fullStr |
Nonribosomal Peptides, Key Biocontrol Components for Pseudomonas fluorescens In5, Isolated from a Greenlandic Suppressive Soil |
title_full_unstemmed |
Nonribosomal Peptides, Key Biocontrol Components for Pseudomonas fluorescens In5, Isolated from a Greenlandic Suppressive Soil |
title_sort |
nonribosomal peptides, key biocontrol components for pseudomonas fluorescens in5, isolated from a greenlandic suppressive soil |
publisher |
American Society for Microbiology |
publishDate |
2015 |
url |
http://dx.doi.org/10.1128/mbio.00079-15 https://journals.asm.org/doi/pdf/10.1128/mBio.00079-15 |
genre |
Greenland greenlandic |
genre_facet |
Greenland greenlandic |
op_source |
mBio volume 6, issue 2 ISSN 2161-2129 2150-7511 |
op_rights |
http://creativecommons.org/licenses/by-nc-sa/3.0/ https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/mbio.00079-15 |
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mBio |
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6 |
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1810447158551248896 |