Comparative Antigenicity and Immunogenicity of Hepadnavirus Core Proteins
ABSTRACT The hepatitis B virus core protein (HBcAg) is a uniquely immunogenic particulate antigen and as such has been used as a vaccine carrier platform. The use of other hepadnavirus core proteins as vaccine carriers has not been explored. To determine whether the rodent hepadnavirus core proteins...
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crasmicro:10.1128/jvi.79.21.13641-13655.2005 2023-06-11T04:09:40+02:00 Comparative Antigenicity and Immunogenicity of Hepadnavirus Core Proteins Billaud, Jean-Noel Peterson, Darrell Schödel, Florian Chen, Antony Sallberg, Matti Garduno, Fermin Goldstein, Phillip McDowell, Wendy Hughes, Janice Jones, Joyce Milich, David 2005 http://dx.doi.org/10.1128/jvi.79.21.13641-13655.2005 https://journals.asm.org/doi/pdf/10.1128/JVI.79.21.13641-13655.2005 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Journal of Virology volume 79, issue 21, page 13641-13655 ISSN 0022-538X 1098-5514 Virology Insect Science Immunology Microbiology journal-article 2005 crasmicro https://doi.org/10.1128/jvi.79.21.13641-13655.2005 2023-05-28T20:55:47Z ABSTRACT The hepatitis B virus core protein (HBcAg) is a uniquely immunogenic particulate antigen and as such has been used as a vaccine carrier platform. The use of other hepadnavirus core proteins as vaccine carriers has not been explored. To determine whether the rodent hepadnavirus core proteins derived from the woodchuck (WHcAg), ground squirrel (GScAg), and arctic squirrel (AScAg) viruses possess immunogen characteristics similar to those of HBcAg, comparative antigenicity and immunogenicity studies were performed. The results indicate that (i) the rodent core proteins are equal in immunogenicity to or more immunogenic than HBcAg at the B-cell and T-cell levels; (ii) major histocompatibility complex (MHC) genes influence the immune response to the rodent core proteins (however, nonresponder haplotypes were not identified); (iii) WHcAg can behave as a T-cell-independent antigen in athymic mice; (iv) the rodent core proteins are not significantly cross-reactive with the HBcAg at the antibody level (however, the nonparticulate “eAgs” do appear to be cross-reactive); (v) the rodent core proteins are only partially cross-reactive with HBcAg at the CD4 + T-cell level, depending on MHC haplotype; and (vi) the rodent core proteins are competent to function as vaccine carrier platforms for heterologous, B-cell epitopes. These results have implications for the selection of an optimal hepadnavirus core protein for vaccine design, especially in view of the “preexisting” immunity problem that is inherent in the use of HBcAg for human vaccine development. Article in Journal/Newspaper Arctic ASM Journals (American Society for Microbiology - via Crossref) Arctic Journal of Virology 79 21 13641 13655 |
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Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Virology Insect Science Immunology Microbiology |
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Virology Insect Science Immunology Microbiology Billaud, Jean-Noel Peterson, Darrell Schödel, Florian Chen, Antony Sallberg, Matti Garduno, Fermin Goldstein, Phillip McDowell, Wendy Hughes, Janice Jones, Joyce Milich, David Comparative Antigenicity and Immunogenicity of Hepadnavirus Core Proteins |
topic_facet |
Virology Insect Science Immunology Microbiology |
description |
ABSTRACT The hepatitis B virus core protein (HBcAg) is a uniquely immunogenic particulate antigen and as such has been used as a vaccine carrier platform. The use of other hepadnavirus core proteins as vaccine carriers has not been explored. To determine whether the rodent hepadnavirus core proteins derived from the woodchuck (WHcAg), ground squirrel (GScAg), and arctic squirrel (AScAg) viruses possess immunogen characteristics similar to those of HBcAg, comparative antigenicity and immunogenicity studies were performed. The results indicate that (i) the rodent core proteins are equal in immunogenicity to or more immunogenic than HBcAg at the B-cell and T-cell levels; (ii) major histocompatibility complex (MHC) genes influence the immune response to the rodent core proteins (however, nonresponder haplotypes were not identified); (iii) WHcAg can behave as a T-cell-independent antigen in athymic mice; (iv) the rodent core proteins are not significantly cross-reactive with the HBcAg at the antibody level (however, the nonparticulate “eAgs” do appear to be cross-reactive); (v) the rodent core proteins are only partially cross-reactive with HBcAg at the CD4 + T-cell level, depending on MHC haplotype; and (vi) the rodent core proteins are competent to function as vaccine carrier platforms for heterologous, B-cell epitopes. These results have implications for the selection of an optimal hepadnavirus core protein for vaccine design, especially in view of the “preexisting” immunity problem that is inherent in the use of HBcAg for human vaccine development. |
format |
Article in Journal/Newspaper |
author |
Billaud, Jean-Noel Peterson, Darrell Schödel, Florian Chen, Antony Sallberg, Matti Garduno, Fermin Goldstein, Phillip McDowell, Wendy Hughes, Janice Jones, Joyce Milich, David |
author_facet |
Billaud, Jean-Noel Peterson, Darrell Schödel, Florian Chen, Antony Sallberg, Matti Garduno, Fermin Goldstein, Phillip McDowell, Wendy Hughes, Janice Jones, Joyce Milich, David |
author_sort |
Billaud, Jean-Noel |
title |
Comparative Antigenicity and Immunogenicity of Hepadnavirus Core Proteins |
title_short |
Comparative Antigenicity and Immunogenicity of Hepadnavirus Core Proteins |
title_full |
Comparative Antigenicity and Immunogenicity of Hepadnavirus Core Proteins |
title_fullStr |
Comparative Antigenicity and Immunogenicity of Hepadnavirus Core Proteins |
title_full_unstemmed |
Comparative Antigenicity and Immunogenicity of Hepadnavirus Core Proteins |
title_sort |
comparative antigenicity and immunogenicity of hepadnavirus core proteins |
publisher |
American Society for Microbiology |
publishDate |
2005 |
url |
http://dx.doi.org/10.1128/jvi.79.21.13641-13655.2005 https://journals.asm.org/doi/pdf/10.1128/JVI.79.21.13641-13655.2005 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Virology volume 79, issue 21, page 13641-13655 ISSN 0022-538X 1098-5514 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/jvi.79.21.13641-13655.2005 |
container_title |
Journal of Virology |
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79 |
container_issue |
21 |
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13641 |
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13655 |
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1768383628735152128 |