Comparative Genomic Analysis of Hyperthermophilic Archaeal Fuselloviridae Viruses
ABSTRACT The complete genome sequences of two Sulfolobus spindle-shaped viruses (SSVs) from acidic hot springs in Kamchatka (Russia) and Yellowstone National Park (United States) have been determined. These nonlytic temperate viruses were isolated from hyperthermophilic Sulfolobus hosts, and both vi...
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American Society for Microbiology
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Online Access: | http://dx.doi.org/10.1128/jvi.78.4.1954-1961.2004 https://journals.asm.org/doi/pdf/10.1128/JVI.78.4.1954-1961.2004 |
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crasmicro:10.1128/jvi.78.4.1954-1961.2004 2024-06-23T07:54:03+00:00 Comparative Genomic Analysis of Hyperthermophilic Archaeal Fuselloviridae Viruses Wiedenheft, Blake Stedman, Kenneth Roberto, Francisco Willits, Deborah Gleske, Anne-Kathrin Zoeller, Luisa Snyder, Jamie Douglas, Trevor Young, Mark 2004 http://dx.doi.org/10.1128/jvi.78.4.1954-1961.2004 https://journals.asm.org/doi/pdf/10.1128/JVI.78.4.1954-1961.2004 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Journal of Virology volume 78, issue 4, page 1954-1961 ISSN 0022-538X 1098-5514 journal-article 2004 crasmicro https://doi.org/10.1128/jvi.78.4.1954-1961.2004 2024-06-10T04:07:22Z ABSTRACT The complete genome sequences of two Sulfolobus spindle-shaped viruses (SSVs) from acidic hot springs in Kamchatka (Russia) and Yellowstone National Park (United States) have been determined. These nonlytic temperate viruses were isolated from hyperthermophilic Sulfolobus hosts, and both viruses share the spindle-shaped morphology characteristic of the Fuselloviridae family. These two genomes, in combination with the previously determined SSV1 genome from Japan and the SSV2 genome from Iceland, have allowed us to carry out a phylogenetic comparison of these geographically distributed hyperthermal viruses. Each virus contains a circular double-stranded DNA genome of ∼15 kbp with approximately 34 open reading frames (ORFs). These Fusellovirus ORFs show little or no similarity to genes in the public databases. In contrast, 18 ORFs are common to all four isolates and may represent the minimal gene set defining this viral group. In general, ORFs on one half of the genome are colinear and highly conserved, while ORFs on the other half are not. One shared ORF among all four genomes is an integrase of the tyrosine recombinase family. All four viral genomes integrate into their host tRNA genes. The specific tRNA gene used for integration varies, and one genome integrates into multiple loci. Several unique ORFs are found in the genome of each isolate. Article in Journal/Newspaper Iceland Kamchatka ASM Journals (American Society for Microbiology) Journal of Virology 78 4 1954 1961 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT The complete genome sequences of two Sulfolobus spindle-shaped viruses (SSVs) from acidic hot springs in Kamchatka (Russia) and Yellowstone National Park (United States) have been determined. These nonlytic temperate viruses were isolated from hyperthermophilic Sulfolobus hosts, and both viruses share the spindle-shaped morphology characteristic of the Fuselloviridae family. These two genomes, in combination with the previously determined SSV1 genome from Japan and the SSV2 genome from Iceland, have allowed us to carry out a phylogenetic comparison of these geographically distributed hyperthermal viruses. Each virus contains a circular double-stranded DNA genome of ∼15 kbp with approximately 34 open reading frames (ORFs). These Fusellovirus ORFs show little or no similarity to genes in the public databases. In contrast, 18 ORFs are common to all four isolates and may represent the minimal gene set defining this viral group. In general, ORFs on one half of the genome are colinear and highly conserved, while ORFs on the other half are not. One shared ORF among all four genomes is an integrase of the tyrosine recombinase family. All four viral genomes integrate into their host tRNA genes. The specific tRNA gene used for integration varies, and one genome integrates into multiple loci. Several unique ORFs are found in the genome of each isolate. |
format |
Article in Journal/Newspaper |
author |
Wiedenheft, Blake Stedman, Kenneth Roberto, Francisco Willits, Deborah Gleske, Anne-Kathrin Zoeller, Luisa Snyder, Jamie Douglas, Trevor Young, Mark |
spellingShingle |
Wiedenheft, Blake Stedman, Kenneth Roberto, Francisco Willits, Deborah Gleske, Anne-Kathrin Zoeller, Luisa Snyder, Jamie Douglas, Trevor Young, Mark Comparative Genomic Analysis of Hyperthermophilic Archaeal Fuselloviridae Viruses |
author_facet |
Wiedenheft, Blake Stedman, Kenneth Roberto, Francisco Willits, Deborah Gleske, Anne-Kathrin Zoeller, Luisa Snyder, Jamie Douglas, Trevor Young, Mark |
author_sort |
Wiedenheft, Blake |
title |
Comparative Genomic Analysis of Hyperthermophilic Archaeal Fuselloviridae Viruses |
title_short |
Comparative Genomic Analysis of Hyperthermophilic Archaeal Fuselloviridae Viruses |
title_full |
Comparative Genomic Analysis of Hyperthermophilic Archaeal Fuselloviridae Viruses |
title_fullStr |
Comparative Genomic Analysis of Hyperthermophilic Archaeal Fuselloviridae Viruses |
title_full_unstemmed |
Comparative Genomic Analysis of Hyperthermophilic Archaeal Fuselloviridae Viruses |
title_sort |
comparative genomic analysis of hyperthermophilic archaeal fuselloviridae viruses |
publisher |
American Society for Microbiology |
publishDate |
2004 |
url |
http://dx.doi.org/10.1128/jvi.78.4.1954-1961.2004 https://journals.asm.org/doi/pdf/10.1128/JVI.78.4.1954-1961.2004 |
genre |
Iceland Kamchatka |
genre_facet |
Iceland Kamchatka |
op_source |
Journal of Virology volume 78, issue 4, page 1954-1961 ISSN 0022-538X 1098-5514 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/jvi.78.4.1954-1961.2004 |
container_title |
Journal of Virology |
container_volume |
78 |
container_issue |
4 |
container_start_page |
1954 |
op_container_end_page |
1961 |
_version_ |
1802645992750710784 |