Inhibition of Infectious Pancreatic Necrosis Virus Replication by Atlantic Salmon Mx1 Protein
ABSTRACT Mx proteins form a family of interferon (IFN)-induced GTPases with potent antiviral activity against various single-stranded RNA viruses in mammals and chickens. In fish, alpha/beta IFN has been reported to inhibit the replication of infectious pancreatic necrosis virus (IPNV), but the mode...
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crasmicro:10.1128/jvi.78.15.7938-7944.2004 2024-09-15T17:56:07+00:00 Inhibition of Infectious Pancreatic Necrosis Virus Replication by Atlantic Salmon Mx1 Protein Larsen, Rannveig Røkenes, Torunn P. Robertsen, Børre 2004 http://dx.doi.org/10.1128/jvi.78.15.7938-7944.2004 https://journals.asm.org/doi/pdf/10.1128/JVI.78.15.7938-7944.2004 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Journal of Virology volume 78, issue 15, page 7938-7944 ISSN 0022-538X 1098-5514 journal-article 2004 crasmicro https://doi.org/10.1128/jvi.78.15.7938-7944.2004 2024-08-19T04:06:13Z ABSTRACT Mx proteins form a family of interferon (IFN)-induced GTPases with potent antiviral activity against various single-stranded RNA viruses in mammals and chickens. In fish, alpha/beta IFN has been reported to inhibit the replication of infectious pancreatic necrosis virus (IPNV), but the mode of action has not been elucidated. A correlation between the inhibition of IPNV and Mx protein expression has, however, been observed. To examine whether Atlantic salmon Mx1 protein (ASMx1) possesses antiviral activity against IPNV, CHSE-214 cells constitutively expressing ASMx1 were established. ASMx1 appeared to be localized in the cytoplasm. The ASMx1-expressing clone selected showed a severely reduced IPNV-induced cytopathic effect, which was confirmed by a 500-fold reduction in virus yield. The antiviral activity against IPNV was further confirmed by the inhibition of virus protein synthesis and the reduced accumulation of virus transcripts. The present work further adds to the body of evidence which suggests that antiviral activity is a major functional role of vertebrate Mx proteins. Moreover, the list of viruses inhibited by Mx proteins is extended to include double-stranded RNA viruses. Article in Journal/Newspaper Atlantic salmon ASM Journals (American Society for Microbiology) Journal of Virology 78 15 7938 7944 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT Mx proteins form a family of interferon (IFN)-induced GTPases with potent antiviral activity against various single-stranded RNA viruses in mammals and chickens. In fish, alpha/beta IFN has been reported to inhibit the replication of infectious pancreatic necrosis virus (IPNV), but the mode of action has not been elucidated. A correlation between the inhibition of IPNV and Mx protein expression has, however, been observed. To examine whether Atlantic salmon Mx1 protein (ASMx1) possesses antiviral activity against IPNV, CHSE-214 cells constitutively expressing ASMx1 were established. ASMx1 appeared to be localized in the cytoplasm. The ASMx1-expressing clone selected showed a severely reduced IPNV-induced cytopathic effect, which was confirmed by a 500-fold reduction in virus yield. The antiviral activity against IPNV was further confirmed by the inhibition of virus protein synthesis and the reduced accumulation of virus transcripts. The present work further adds to the body of evidence which suggests that antiviral activity is a major functional role of vertebrate Mx proteins. Moreover, the list of viruses inhibited by Mx proteins is extended to include double-stranded RNA viruses. |
format |
Article in Journal/Newspaper |
author |
Larsen, Rannveig Røkenes, Torunn P. Robertsen, Børre |
spellingShingle |
Larsen, Rannveig Røkenes, Torunn P. Robertsen, Børre Inhibition of Infectious Pancreatic Necrosis Virus Replication by Atlantic Salmon Mx1 Protein |
author_facet |
Larsen, Rannveig Røkenes, Torunn P. Robertsen, Børre |
author_sort |
Larsen, Rannveig |
title |
Inhibition of Infectious Pancreatic Necrosis Virus Replication by Atlantic Salmon Mx1 Protein |
title_short |
Inhibition of Infectious Pancreatic Necrosis Virus Replication by Atlantic Salmon Mx1 Protein |
title_full |
Inhibition of Infectious Pancreatic Necrosis Virus Replication by Atlantic Salmon Mx1 Protein |
title_fullStr |
Inhibition of Infectious Pancreatic Necrosis Virus Replication by Atlantic Salmon Mx1 Protein |
title_full_unstemmed |
Inhibition of Infectious Pancreatic Necrosis Virus Replication by Atlantic Salmon Mx1 Protein |
title_sort |
inhibition of infectious pancreatic necrosis virus replication by atlantic salmon mx1 protein |
publisher |
American Society for Microbiology |
publishDate |
2004 |
url |
http://dx.doi.org/10.1128/jvi.78.15.7938-7944.2004 https://journals.asm.org/doi/pdf/10.1128/JVI.78.15.7938-7944.2004 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of Virology volume 78, issue 15, page 7938-7944 ISSN 0022-538X 1098-5514 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/jvi.78.15.7938-7944.2004 |
container_title |
Journal of Virology |
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78 |
container_issue |
15 |
container_start_page |
7938 |
op_container_end_page |
7944 |
_version_ |
1810432325220040704 |