The Putative Polymerase Sequence of Infectious Salmon Anemia Virus Suggests a New Genus within the Orthomyxoviridae
ABSTRACT The infectious salmon anemia virus (ISAV) is an orthomyxovirus-like virus infecting teleosts. The disease caused by this virus has had major economic consequences for the Atlantic salmon farming industry in Norway, Canada, and Scotland. In this work, we report the cloning and sequencing of...
Published in: | Journal of Virology |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
American Society for Microbiology
1999
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1128/jvi.73.3.2136-2142.1999 https://journals.asm.org/doi/pdf/10.1128/JVI.73.3.2136-2142.1999 |
id |
crasmicro:10.1128/jvi.73.3.2136-2142.1999 |
---|---|
record_format |
openpolar |
spelling |
crasmicro:10.1128/jvi.73.3.2136-2142.1999 2024-09-30T14:32:32+00:00 The Putative Polymerase Sequence of Infectious Salmon Anemia Virus Suggests a New Genus within the Orthomyxoviridae Krossøy, Bjørn Hordvik, Ivar Nilsen, Frank Nylund, Are Endresen, Curt 1999 http://dx.doi.org/10.1128/jvi.73.3.2136-2142.1999 https://journals.asm.org/doi/pdf/10.1128/JVI.73.3.2136-2142.1999 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Journal of Virology volume 73, issue 3, page 2136-2142 ISSN 0022-538X 1098-5514 journal-article 1999 crasmicro https://doi.org/10.1128/jvi.73.3.2136-2142.1999 2024-09-17T04:08:24Z ABSTRACT The infectious salmon anemia virus (ISAV) is an orthomyxovirus-like virus infecting teleosts. The disease caused by this virus has had major economic consequences for the Atlantic salmon farming industry in Norway, Canada, and Scotland. In this work, we report the cloning and sequencing of an ISAV-specific cDNA comprising 2,245 bp with an open reading frame coding for a predicted protein with a calculated molecular weight of 80.5 kDa. The putative protein sequence shows the core polymerase motifs characteristic of all viral RNA-dependent RNA polymerases. Comparison of the conserved motifs with the corresponding regions of other segmented negative-stranded RNA viruses shows a closer relationship with members of the Orthomyxoviridae than with viruses in other families. The putative ISAV polymerase protein (PB1) has a length of 708 amino acids, a charge of +22 at neutral pH, and a pI of 9.9, which are consistent with the properties of the PB1 proteins of other members of the family. Calculations of the distances between the different PB1 proteins indicate that the ISAV is distantly related to the other members of the family but more closely related to the influenza viruses than to the Thogoto viruses. Based on these and previously published results, we propose that the ISAV comprises a new, fifth genus in the Orthomyxoviridae . Article in Journal/Newspaper Atlantic salmon ASM Journals (American Society for Microbiology) Canada Norway Journal of Virology 73 3 2136 2142 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
description |
ABSTRACT The infectious salmon anemia virus (ISAV) is an orthomyxovirus-like virus infecting teleosts. The disease caused by this virus has had major economic consequences for the Atlantic salmon farming industry in Norway, Canada, and Scotland. In this work, we report the cloning and sequencing of an ISAV-specific cDNA comprising 2,245 bp with an open reading frame coding for a predicted protein with a calculated molecular weight of 80.5 kDa. The putative protein sequence shows the core polymerase motifs characteristic of all viral RNA-dependent RNA polymerases. Comparison of the conserved motifs with the corresponding regions of other segmented negative-stranded RNA viruses shows a closer relationship with members of the Orthomyxoviridae than with viruses in other families. The putative ISAV polymerase protein (PB1) has a length of 708 amino acids, a charge of +22 at neutral pH, and a pI of 9.9, which are consistent with the properties of the PB1 proteins of other members of the family. Calculations of the distances between the different PB1 proteins indicate that the ISAV is distantly related to the other members of the family but more closely related to the influenza viruses than to the Thogoto viruses. Based on these and previously published results, we propose that the ISAV comprises a new, fifth genus in the Orthomyxoviridae . |
format |
Article in Journal/Newspaper |
author |
Krossøy, Bjørn Hordvik, Ivar Nilsen, Frank Nylund, Are Endresen, Curt |
spellingShingle |
Krossøy, Bjørn Hordvik, Ivar Nilsen, Frank Nylund, Are Endresen, Curt The Putative Polymerase Sequence of Infectious Salmon Anemia Virus Suggests a New Genus within the Orthomyxoviridae |
author_facet |
Krossøy, Bjørn Hordvik, Ivar Nilsen, Frank Nylund, Are Endresen, Curt |
author_sort |
Krossøy, Bjørn |
title |
The Putative Polymerase Sequence of Infectious Salmon Anemia Virus Suggests a New Genus within the Orthomyxoviridae |
title_short |
The Putative Polymerase Sequence of Infectious Salmon Anemia Virus Suggests a New Genus within the Orthomyxoviridae |
title_full |
The Putative Polymerase Sequence of Infectious Salmon Anemia Virus Suggests a New Genus within the Orthomyxoviridae |
title_fullStr |
The Putative Polymerase Sequence of Infectious Salmon Anemia Virus Suggests a New Genus within the Orthomyxoviridae |
title_full_unstemmed |
The Putative Polymerase Sequence of Infectious Salmon Anemia Virus Suggests a New Genus within the Orthomyxoviridae |
title_sort |
putative polymerase sequence of infectious salmon anemia virus suggests a new genus within the orthomyxoviridae |
publisher |
American Society for Microbiology |
publishDate |
1999 |
url |
http://dx.doi.org/10.1128/jvi.73.3.2136-2142.1999 https://journals.asm.org/doi/pdf/10.1128/JVI.73.3.2136-2142.1999 |
geographic |
Canada Norway |
geographic_facet |
Canada Norway |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of Virology volume 73, issue 3, page 2136-2142 ISSN 0022-538X 1098-5514 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/jvi.73.3.2136-2142.1999 |
container_title |
Journal of Virology |
container_volume |
73 |
container_issue |
3 |
container_start_page |
2136 |
op_container_end_page |
2142 |
_version_ |
1811636676882071552 |