Genomic characterization of the virus causing infectious salmon anemia in Atlantic salmon (Salmo salar L.): an orthomyxo-like virus in a teleost
The genome of infectious salmon anemia virus (ISAV), which infects farmed Atlantic salmon (Salmo salar L.), is characterized here. The virus has an RNA genome, as shown by using specific DNA virus metabolic inhibitors and radioactive in vivo labeling of ISAV nucleic acid. Electrophoresis of [14C]uri...
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American Society for Microbiology
1997
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Online Access: | http://dx.doi.org/10.1128/jvi.71.10.7681-7686.1997 https://journals.asm.org/doi/pdf/10.1128/jvi.71.10.7681-7686.1997 |
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crasmicro:10.1128/jvi.71.10.7681-7686.1997 2024-06-23T07:51:20+00:00 Genomic characterization of the virus causing infectious salmon anemia in Atlantic salmon (Salmo salar L.): an orthomyxo-like virus in a teleost Mjaaland, S Rimstad, E Falk, K Dannevig, B H 1997 http://dx.doi.org/10.1128/jvi.71.10.7681-7686.1997 https://journals.asm.org/doi/pdf/10.1128/jvi.71.10.7681-7686.1997 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Journal of Virology volume 71, issue 10, page 7681-7686 ISSN 0022-538X 1098-5514 journal-article 1997 crasmicro https://doi.org/10.1128/jvi.71.10.7681-7686.1997 2024-06-10T04:07:22Z The genome of infectious salmon anemia virus (ISAV), which infects farmed Atlantic salmon (Salmo salar L.), is characterized here. The virus has an RNA genome, as shown by using specific DNA virus metabolic inhibitors and radioactive in vivo labeling of ISAV nucleic acid. Electrophoresis of [14C]uridine-labeled ISAV RNA revealed that the ISAV genome is segmented. The genome consists of eight segments that range from 1.0 to 2.3 kb, with a total molecular size of approximately 14.5 kb. One ISAV-specific molecular clone, corresponding to the smallest genome segment, was obtained by cDNA cloning of mRNA from an ISAV-infected cell culture. This clone gave a positive hybridization signal on Northern blots of pelleted ISAV. Pretreatment of the ISAV pellet with RNase A resulted in the disappearance of the positive hybridization signal, demonstrating that the genome is single stranded. Reverse transcriptase PCR with primers corresponding to sequences from the molecular clone and target RNA from ISAV-infected and noninfected fish tissues gave specific positive reactions. Alignments of the nucleotide sequence of the molecular clone did not reveal significant homology with any other available sequence in databases. However, the data presented here, together with morphological and replicational properties previously described, indicate that ISAV has a strong resemblance to members of the Orthomyxoviridae family. This is the first thoroughly characterized orthomyxo-like virus isolated from a teleost. Article in Journal/Newspaper Atlantic salmon Salmo salar ASM Journals (American Society for Microbiology) Journal of Virology 71 10 7681 7686 |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
The genome of infectious salmon anemia virus (ISAV), which infects farmed Atlantic salmon (Salmo salar L.), is characterized here. The virus has an RNA genome, as shown by using specific DNA virus metabolic inhibitors and radioactive in vivo labeling of ISAV nucleic acid. Electrophoresis of [14C]uridine-labeled ISAV RNA revealed that the ISAV genome is segmented. The genome consists of eight segments that range from 1.0 to 2.3 kb, with a total molecular size of approximately 14.5 kb. One ISAV-specific molecular clone, corresponding to the smallest genome segment, was obtained by cDNA cloning of mRNA from an ISAV-infected cell culture. This clone gave a positive hybridization signal on Northern blots of pelleted ISAV. Pretreatment of the ISAV pellet with RNase A resulted in the disappearance of the positive hybridization signal, demonstrating that the genome is single stranded. Reverse transcriptase PCR with primers corresponding to sequences from the molecular clone and target RNA from ISAV-infected and noninfected fish tissues gave specific positive reactions. Alignments of the nucleotide sequence of the molecular clone did not reveal significant homology with any other available sequence in databases. However, the data presented here, together with morphological and replicational properties previously described, indicate that ISAV has a strong resemblance to members of the Orthomyxoviridae family. This is the first thoroughly characterized orthomyxo-like virus isolated from a teleost. |
format |
Article in Journal/Newspaper |
author |
Mjaaland, S Rimstad, E Falk, K Dannevig, B H |
spellingShingle |
Mjaaland, S Rimstad, E Falk, K Dannevig, B H Genomic characterization of the virus causing infectious salmon anemia in Atlantic salmon (Salmo salar L.): an orthomyxo-like virus in a teleost |
author_facet |
Mjaaland, S Rimstad, E Falk, K Dannevig, B H |
author_sort |
Mjaaland, S |
title |
Genomic characterization of the virus causing infectious salmon anemia in Atlantic salmon (Salmo salar L.): an orthomyxo-like virus in a teleost |
title_short |
Genomic characterization of the virus causing infectious salmon anemia in Atlantic salmon (Salmo salar L.): an orthomyxo-like virus in a teleost |
title_full |
Genomic characterization of the virus causing infectious salmon anemia in Atlantic salmon (Salmo salar L.): an orthomyxo-like virus in a teleost |
title_fullStr |
Genomic characterization of the virus causing infectious salmon anemia in Atlantic salmon (Salmo salar L.): an orthomyxo-like virus in a teleost |
title_full_unstemmed |
Genomic characterization of the virus causing infectious salmon anemia in Atlantic salmon (Salmo salar L.): an orthomyxo-like virus in a teleost |
title_sort |
genomic characterization of the virus causing infectious salmon anemia in atlantic salmon (salmo salar l.): an orthomyxo-like virus in a teleost |
publisher |
American Society for Microbiology |
publishDate |
1997 |
url |
http://dx.doi.org/10.1128/jvi.71.10.7681-7686.1997 https://journals.asm.org/doi/pdf/10.1128/jvi.71.10.7681-7686.1997 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Virology volume 71, issue 10, page 7681-7686 ISSN 0022-538X 1098-5514 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/jvi.71.10.7681-7686.1997 |
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Journal of Virology |
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71 |
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10 |
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7681 |
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7686 |
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1802642391002251264 |