A Single Dose of an Avian H3N8 Influenza Virus Vaccine Is Highly Immunogenic and Efficacious against a Recently Emerged Seal Influenza Virus in Mice and Ferrets
ABSTRACT H3N8 influenza viruses are a commonly found subtype in wild birds, usually causing mild or no disease in infected birds. However, they have crossed the species barrier and have been associated with outbreaks in dogs, pigs, donkeys, and seals and therefore pose a threat to humans. A live att...
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Online Access: | http://dx.doi.org/10.1128/jvi.00280-15 https://journals.asm.org/doi/pdf/10.1128/JVI.00280-15 |
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crasmicro:10.1128/jvi.00280-15 2024-03-03T08:45:08+00:00 A Single Dose of an Avian H3N8 Influenza Virus Vaccine Is Highly Immunogenic and Efficacious against a Recently Emerged Seal Influenza Virus in Mice and Ferrets Baz, Mariana Paskel, Myeisha Matsuoka, Yumiko Zengel, James R. Cheng, Xing Treanor, John J. Jin, Hong Subbarao, Kanta Perlman, S. 2015 http://dx.doi.org/10.1128/jvi.00280-15 https://journals.asm.org/doi/pdf/10.1128/JVI.00280-15 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Journal of Virology volume 89, issue 13, page 6907-6917 ISSN 0022-538X 1098-5514 Virology Insect Science Immunology Microbiology journal-article 2015 crasmicro https://doi.org/10.1128/jvi.00280-15 2024-02-03T23:13:12Z ABSTRACT H3N8 influenza viruses are a commonly found subtype in wild birds, usually causing mild or no disease in infected birds. However, they have crossed the species barrier and have been associated with outbreaks in dogs, pigs, donkeys, and seals and therefore pose a threat to humans. A live attenuated, cold-adapted ( ca ) H3N8 vaccine virus was generated by reverse genetics using the wild-type (wt) hemagglutinin (HA) and neuraminidase (NA) genes from the A/blue-winged teal/Texas/Sg-00079/2007 (H3N8) (tl/TX/079/07) wt virus and the six internal protein gene segments from the ca influenza A virus vaccine donor strain, A/Ann Arbor/6/60 ca (H2N2), the backbone of the licensed seasonal live attenuated influenza vaccine. One dose of the tl/TX/079/07 ca vaccine induced a robust neutralizing antibody response against the homologous (tl/TX/079/07) and two heterologous influenza viruses, including the recently emerged A/harbor seal/New Hampshire/179629/2011 (H3N8) and A/northern pintail/Alaska/44228-129/2006 (H3N8) viruses, and conferred robust protection against the homologous and heterologous influenza viruses. We also analyzed human sera against the tl/TX/079/07 H3N8 avian influenza virus and observed low but detectable antibody reactivity in elderly subjects, suggesting that older H3N2 influenza viruses confer some cross-reactive antibody. The latter observation was confirmed in a ferret study. The safety, immunogenicity, and efficacy of the tl/TX/079/07 ca vaccine in mice and ferrets support further evaluation of this vaccine in humans for use in the event of transmission of an H3N8 avian influenza virus to humans. The human and ferret serology data suggest that a single dose of the vaccine may be sufficient in older subjects. IMPORTANCE Although natural infection of humans with an avian H3N8 influenza virus has not yet been reported, this influenza virus subtype has already crossed the species barrier and productively infected mammals. Pandemic preparedness is an important public health priority. Therefore, we ... Article in Journal/Newspaper harbor seal Alaska ASM Journals (American Society for Microbiology) Journal of Virology 89 13 6907 6917 |
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Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
topic |
Virology Insect Science Immunology Microbiology |
spellingShingle |
Virology Insect Science Immunology Microbiology Baz, Mariana Paskel, Myeisha Matsuoka, Yumiko Zengel, James R. Cheng, Xing Treanor, John J. Jin, Hong Subbarao, Kanta A Single Dose of an Avian H3N8 Influenza Virus Vaccine Is Highly Immunogenic and Efficacious against a Recently Emerged Seal Influenza Virus in Mice and Ferrets |
topic_facet |
Virology Insect Science Immunology Microbiology |
description |
ABSTRACT H3N8 influenza viruses are a commonly found subtype in wild birds, usually causing mild or no disease in infected birds. However, they have crossed the species barrier and have been associated with outbreaks in dogs, pigs, donkeys, and seals and therefore pose a threat to humans. A live attenuated, cold-adapted ( ca ) H3N8 vaccine virus was generated by reverse genetics using the wild-type (wt) hemagglutinin (HA) and neuraminidase (NA) genes from the A/blue-winged teal/Texas/Sg-00079/2007 (H3N8) (tl/TX/079/07) wt virus and the six internal protein gene segments from the ca influenza A virus vaccine donor strain, A/Ann Arbor/6/60 ca (H2N2), the backbone of the licensed seasonal live attenuated influenza vaccine. One dose of the tl/TX/079/07 ca vaccine induced a robust neutralizing antibody response against the homologous (tl/TX/079/07) and two heterologous influenza viruses, including the recently emerged A/harbor seal/New Hampshire/179629/2011 (H3N8) and A/northern pintail/Alaska/44228-129/2006 (H3N8) viruses, and conferred robust protection against the homologous and heterologous influenza viruses. We also analyzed human sera against the tl/TX/079/07 H3N8 avian influenza virus and observed low but detectable antibody reactivity in elderly subjects, suggesting that older H3N2 influenza viruses confer some cross-reactive antibody. The latter observation was confirmed in a ferret study. The safety, immunogenicity, and efficacy of the tl/TX/079/07 ca vaccine in mice and ferrets support further evaluation of this vaccine in humans for use in the event of transmission of an H3N8 avian influenza virus to humans. The human and ferret serology data suggest that a single dose of the vaccine may be sufficient in older subjects. IMPORTANCE Although natural infection of humans with an avian H3N8 influenza virus has not yet been reported, this influenza virus subtype has already crossed the species barrier and productively infected mammals. Pandemic preparedness is an important public health priority. Therefore, we ... |
author2 |
Perlman, S. |
format |
Article in Journal/Newspaper |
author |
Baz, Mariana Paskel, Myeisha Matsuoka, Yumiko Zengel, James R. Cheng, Xing Treanor, John J. Jin, Hong Subbarao, Kanta |
author_facet |
Baz, Mariana Paskel, Myeisha Matsuoka, Yumiko Zengel, James R. Cheng, Xing Treanor, John J. Jin, Hong Subbarao, Kanta |
author_sort |
Baz, Mariana |
title |
A Single Dose of an Avian H3N8 Influenza Virus Vaccine Is Highly Immunogenic and Efficacious against a Recently Emerged Seal Influenza Virus in Mice and Ferrets |
title_short |
A Single Dose of an Avian H3N8 Influenza Virus Vaccine Is Highly Immunogenic and Efficacious against a Recently Emerged Seal Influenza Virus in Mice and Ferrets |
title_full |
A Single Dose of an Avian H3N8 Influenza Virus Vaccine Is Highly Immunogenic and Efficacious against a Recently Emerged Seal Influenza Virus in Mice and Ferrets |
title_fullStr |
A Single Dose of an Avian H3N8 Influenza Virus Vaccine Is Highly Immunogenic and Efficacious against a Recently Emerged Seal Influenza Virus in Mice and Ferrets |
title_full_unstemmed |
A Single Dose of an Avian H3N8 Influenza Virus Vaccine Is Highly Immunogenic and Efficacious against a Recently Emerged Seal Influenza Virus in Mice and Ferrets |
title_sort |
single dose of an avian h3n8 influenza virus vaccine is highly immunogenic and efficacious against a recently emerged seal influenza virus in mice and ferrets |
publisher |
American Society for Microbiology |
publishDate |
2015 |
url |
http://dx.doi.org/10.1128/jvi.00280-15 https://journals.asm.org/doi/pdf/10.1128/JVI.00280-15 |
genre |
harbor seal Alaska |
genre_facet |
harbor seal Alaska |
op_source |
Journal of Virology volume 89, issue 13, page 6907-6917 ISSN 0022-538X 1098-5514 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/jvi.00280-15 |
container_title |
Journal of Virology |
container_volume |
89 |
container_issue |
13 |
container_start_page |
6907 |
op_container_end_page |
6917 |
_version_ |
1792500669293264896 |