Molecular polymorphism of the lyme disease agent Borrelia garinii in northern Europe is influenced by a novel enzootic Borrelia focus in the North Atlantic
Lyme disease Borrelia species are distributed in temperate areas of North America and Eurasia. To elucidate the distribution of borreliae in subarctic regions, strains isolated from Ixodes ricinus and Ixodes uriae ticks found on islands in the northern Atlantic and Baltic Sea were molecularly charac...
Published in: | Journal of Clinical Microbiology |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
American Society for Microbiology
1996
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1128/jcm.34.2.364-368.1996 https://journals.asm.org/doi/pdf/10.1128/jcm.34.2.364-368.1996 |
id |
crasmicro:10.1128/jcm.34.2.364-368.1996 |
---|---|
record_format |
openpolar |
spelling |
crasmicro:10.1128/jcm.34.2.364-368.1996 2023-05-15T17:28:22+02:00 Molecular polymorphism of the lyme disease agent Borrelia garinii in northern Europe is influenced by a novel enzootic Borrelia focus in the North Atlantic Bunikis, J Olsén, B Fingerle, V Bonnedahl, J Wilske, B Bergström, S 1996 http://dx.doi.org/10.1128/jcm.34.2.364-368.1996 https://journals.asm.org/doi/pdf/10.1128/jcm.34.2.364-368.1996 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Journal of Clinical Microbiology volume 34, issue 2, page 364-368 ISSN 0095-1137 1098-660X Microbiology (medical) journal-article 1996 crasmicro https://doi.org/10.1128/jcm.34.2.364-368.1996 2023-01-20T07:32:20Z Lyme disease Borrelia species are distributed in temperate areas of North America and Eurasia. To elucidate the distribution of borreliae in subarctic regions, strains isolated from Ixodes ricinus and Ixodes uriae ticks found on islands in the northern Atlantic and Baltic Sea were molecularly characterized. All isolates were verified as Borrelia garinii by 16S rRNA gene analysis and immunoblotting with monoclonal antibodies specific for the outer surface proteins A and C. Three ribotypes (RTs) of B. garinii were delineated. I. ricinus complex-associated RT1 was phenotypically most heterogeneous. Two newly identified ribotypes were shared by different tick species and conformed to two established OspA serotypes. RT2 was restricted to the islands in the northern Baltic Sea, whereas RT3 was recovered also from ticks found in the North Atlantic. In conclusion, molecular polymorphism of the studied borrelia isolates suggests a complex enzootic potential of B. garinii in northern Europe and implies a novel, seabird tick I. uriae-associated enzootic focus of Lyme disease borreliae in the North Atlantic. Article in Journal/Newspaper North Atlantic Subarctic ASM Journals (American Society for Microbiology - via Crossref) Ospa ENVELOPE(15.258,15.258,68.719,68.719) Journal of Clinical Microbiology 34 2 364 368 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Microbiology (medical) |
spellingShingle |
Microbiology (medical) Bunikis, J Olsén, B Fingerle, V Bonnedahl, J Wilske, B Bergström, S Molecular polymorphism of the lyme disease agent Borrelia garinii in northern Europe is influenced by a novel enzootic Borrelia focus in the North Atlantic |
topic_facet |
Microbiology (medical) |
description |
Lyme disease Borrelia species are distributed in temperate areas of North America and Eurasia. To elucidate the distribution of borreliae in subarctic regions, strains isolated from Ixodes ricinus and Ixodes uriae ticks found on islands in the northern Atlantic and Baltic Sea were molecularly characterized. All isolates were verified as Borrelia garinii by 16S rRNA gene analysis and immunoblotting with monoclonal antibodies specific for the outer surface proteins A and C. Three ribotypes (RTs) of B. garinii were delineated. I. ricinus complex-associated RT1 was phenotypically most heterogeneous. Two newly identified ribotypes were shared by different tick species and conformed to two established OspA serotypes. RT2 was restricted to the islands in the northern Baltic Sea, whereas RT3 was recovered also from ticks found in the North Atlantic. In conclusion, molecular polymorphism of the studied borrelia isolates suggests a complex enzootic potential of B. garinii in northern Europe and implies a novel, seabird tick I. uriae-associated enzootic focus of Lyme disease borreliae in the North Atlantic. |
format |
Article in Journal/Newspaper |
author |
Bunikis, J Olsén, B Fingerle, V Bonnedahl, J Wilske, B Bergström, S |
author_facet |
Bunikis, J Olsén, B Fingerle, V Bonnedahl, J Wilske, B Bergström, S |
author_sort |
Bunikis, J |
title |
Molecular polymorphism of the lyme disease agent Borrelia garinii in northern Europe is influenced by a novel enzootic Borrelia focus in the North Atlantic |
title_short |
Molecular polymorphism of the lyme disease agent Borrelia garinii in northern Europe is influenced by a novel enzootic Borrelia focus in the North Atlantic |
title_full |
Molecular polymorphism of the lyme disease agent Borrelia garinii in northern Europe is influenced by a novel enzootic Borrelia focus in the North Atlantic |
title_fullStr |
Molecular polymorphism of the lyme disease agent Borrelia garinii in northern Europe is influenced by a novel enzootic Borrelia focus in the North Atlantic |
title_full_unstemmed |
Molecular polymorphism of the lyme disease agent Borrelia garinii in northern Europe is influenced by a novel enzootic Borrelia focus in the North Atlantic |
title_sort |
molecular polymorphism of the lyme disease agent borrelia garinii in northern europe is influenced by a novel enzootic borrelia focus in the north atlantic |
publisher |
American Society for Microbiology |
publishDate |
1996 |
url |
http://dx.doi.org/10.1128/jcm.34.2.364-368.1996 https://journals.asm.org/doi/pdf/10.1128/jcm.34.2.364-368.1996 |
long_lat |
ENVELOPE(15.258,15.258,68.719,68.719) |
geographic |
Ospa |
geographic_facet |
Ospa |
genre |
North Atlantic Subarctic |
genre_facet |
North Atlantic Subarctic |
op_source |
Journal of Clinical Microbiology volume 34, issue 2, page 364-368 ISSN 0095-1137 1098-660X |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/jcm.34.2.364-368.1996 |
container_title |
Journal of Clinical Microbiology |
container_volume |
34 |
container_issue |
2 |
container_start_page |
364 |
op_container_end_page |
368 |
_version_ |
1766120992111853568 |