Molecular Epidemiology of Staphylococcus saprophyticus Isolated from Women with Uncomplicated Community-Acquired Urinary Tract Infection
ABSTRACT Staphylococcus saprophyticus is a common cause of urinary tract infections (UTIs) in women. Little is known about the molecular epidemiology of S. saprophyticus UTIs. In the current study, we compared 76 isolates of S. saprophyticus prospectively isolated from women with uncomplicated UTI p...
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crasmicro:10.1128/jcm.02071-06 2024-09-15T18:26:10+00:00 Molecular Epidemiology of Staphylococcus saprophyticus Isolated from Women with Uncomplicated Community-Acquired Urinary Tract Infection Widerström, Micael Wiström, Johan Ferry, Sven Karlsson, Carina Monsen, Tor 2007 http://dx.doi.org/10.1128/jcm.02071-06 https://journals.asm.org/doi/pdf/10.1128/JCM.02071-06 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Journal of Clinical Microbiology volume 45, issue 5, page 1561-1564 ISSN 0095-1137 1098-660X journal-article 2007 crasmicro https://doi.org/10.1128/jcm.02071-06 2024-08-26T04:06:26Z ABSTRACT Staphylococcus saprophyticus is a common cause of urinary tract infections (UTIs) in women. Little is known about the molecular epidemiology of S. saprophyticus UTIs. In the current study, we compared 76 isolates of S. saprophyticus prospectively isolated from women with uncomplicated UTI participating in a randomized placebo-controlled treatment trial performed in northern Sweden from 1995 to 1997 with 50 strains obtained in 2006 from five different locations in northern Europe with pulsed-field gel electrophoresis (PFGE). The aim was to elucidate the molecular epidemiology of this uropathogenic species and to investigate whether specific clones are associated with UTI in women. A total of 47 different PFGE profiles were detected among the 126 analyzed isolates. Ten clusters consisting of 5 to 12 isolates each showing PFGE DNA similarity of >85% were identified. Several clusters of genetically highly related isolates were detected in the original trial as well as among isolates obtained during 2006 from different locations. In the original trial, clonal persistence was found among 16 of 21 (76%) patients examined in the placebo group at follow-up 8 to 10 days after inclusion, indicating a low spontaneous short-time bacteriological cure rate. We conclude that multiple clones of S. saprophyticus were causing lower UTIs in women. The result suggests that some human-pathogenic clones of S. saprophyticus are spread over large geographical distances and that such clones may persist over long periods of time. Article in Journal/Newspaper Northern Sweden ASM Journals (American Society for Microbiology) Journal of Clinical Microbiology 45 5 1561 1564 |
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ASM Journals (American Society for Microbiology) |
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English |
description |
ABSTRACT Staphylococcus saprophyticus is a common cause of urinary tract infections (UTIs) in women. Little is known about the molecular epidemiology of S. saprophyticus UTIs. In the current study, we compared 76 isolates of S. saprophyticus prospectively isolated from women with uncomplicated UTI participating in a randomized placebo-controlled treatment trial performed in northern Sweden from 1995 to 1997 with 50 strains obtained in 2006 from five different locations in northern Europe with pulsed-field gel electrophoresis (PFGE). The aim was to elucidate the molecular epidemiology of this uropathogenic species and to investigate whether specific clones are associated with UTI in women. A total of 47 different PFGE profiles were detected among the 126 analyzed isolates. Ten clusters consisting of 5 to 12 isolates each showing PFGE DNA similarity of >85% were identified. Several clusters of genetically highly related isolates were detected in the original trial as well as among isolates obtained during 2006 from different locations. In the original trial, clonal persistence was found among 16 of 21 (76%) patients examined in the placebo group at follow-up 8 to 10 days after inclusion, indicating a low spontaneous short-time bacteriological cure rate. We conclude that multiple clones of S. saprophyticus were causing lower UTIs in women. The result suggests that some human-pathogenic clones of S. saprophyticus are spread over large geographical distances and that such clones may persist over long periods of time. |
format |
Article in Journal/Newspaper |
author |
Widerström, Micael Wiström, Johan Ferry, Sven Karlsson, Carina Monsen, Tor |
spellingShingle |
Widerström, Micael Wiström, Johan Ferry, Sven Karlsson, Carina Monsen, Tor Molecular Epidemiology of Staphylococcus saprophyticus Isolated from Women with Uncomplicated Community-Acquired Urinary Tract Infection |
author_facet |
Widerström, Micael Wiström, Johan Ferry, Sven Karlsson, Carina Monsen, Tor |
author_sort |
Widerström, Micael |
title |
Molecular Epidemiology of Staphylococcus saprophyticus Isolated from Women with Uncomplicated Community-Acquired Urinary Tract Infection |
title_short |
Molecular Epidemiology of Staphylococcus saprophyticus Isolated from Women with Uncomplicated Community-Acquired Urinary Tract Infection |
title_full |
Molecular Epidemiology of Staphylococcus saprophyticus Isolated from Women with Uncomplicated Community-Acquired Urinary Tract Infection |
title_fullStr |
Molecular Epidemiology of Staphylococcus saprophyticus Isolated from Women with Uncomplicated Community-Acquired Urinary Tract Infection |
title_full_unstemmed |
Molecular Epidemiology of Staphylococcus saprophyticus Isolated from Women with Uncomplicated Community-Acquired Urinary Tract Infection |
title_sort |
molecular epidemiology of staphylococcus saprophyticus isolated from women with uncomplicated community-acquired urinary tract infection |
publisher |
American Society for Microbiology |
publishDate |
2007 |
url |
http://dx.doi.org/10.1128/jcm.02071-06 https://journals.asm.org/doi/pdf/10.1128/JCM.02071-06 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
Journal of Clinical Microbiology volume 45, issue 5, page 1561-1564 ISSN 0095-1137 1098-660X |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/jcm.02071-06 |
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Journal of Clinical Microbiology |
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45 |
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5 |
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1561 |
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1564 |
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1810466617489883136 |