Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada
ABSTRACT Shiga-toxigenic Escherichia coli (STEC) is an important cause of diarrheal disease. The most notorious STEC serotype is O157:H7, which is associated with hemorrhagic colitis and hemolytic-uremic syndrome (HUS). As a result, this serotype is routinely screened for in clinical microbiology la...
Published in: | Journal of Clinical Microbiology |
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crasmicro:10.1128/jcm.01693-10 2024-09-15T18:26:39+00:00 Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada Couturier, Marc Roger Lee, Bonita Zelyas, Nathan Chui, Linda 2011 http://dx.doi.org/10.1128/jcm.01693-10 https://journals.asm.org/doi/pdf/10.1128/JCM.01693-10 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Journal of Clinical Microbiology volume 49, issue 2, page 574-578 ISSN 0095-1137 1098-660X journal-article 2011 crasmicro https://doi.org/10.1128/jcm.01693-10 2024-08-05T04:10:15Z ABSTRACT Shiga-toxigenic Escherichia coli (STEC) is an important cause of diarrheal disease. The most notorious STEC serotype is O157:H7, which is associated with hemorrhagic colitis and hemolytic-uremic syndrome (HUS). As a result, this serotype is routinely screened for in clinical microbiology laboratories. With the bias toward the identification of the O157 serogroup in routine diagnostic processes, non-O157 STEC has been largely underrepresented in the epidemiology of STEC infections. This diagnostic bias is further complicated by the fact that many non-O157 STEC infections cause nonspecific gastroenteritis symptoms reminiscent of enteric viral infections. In this study, real-time PCR was used to amplify Shiga toxin genetic determinants ( stx 1 and stx 2 ) from enriched stool samples that were initially submitted for the testing of enteric viruses in patients with suspected viral gastroenteritis between May and September of 2006, 2007, and 2008 ( n = 2,702). Samples were submitted from the province of Alberta, Yukon, the Northwest Territories, and Nunavut, Canada. A total of 38 samples (1.4%) tested positive for Shiga toxin genes, and 15 isolates were cultured for further characterization. Several of the serotypes identified (O157:H7, O26:HNM, O26:H11, O103:H25, O121:H19, and O145:HNM) have been previously associated with outbreaks and HUS. This study outlines the importance of combining molecular methods with classical culture techniques to enhance the detection of emerging non-O157 as well as O157 serotypes in diarrheal stool samples. Furthermore, atypical diarrhea disease caused by non-O157 STEC can be routinely missed due to screening only for viral agents. Article in Journal/Newspaper Northwest Territories Nunavut Yukon ASM Journals (American Society for Microbiology) Journal of Clinical Microbiology 49 2 574 578 |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT Shiga-toxigenic Escherichia coli (STEC) is an important cause of diarrheal disease. The most notorious STEC serotype is O157:H7, which is associated with hemorrhagic colitis and hemolytic-uremic syndrome (HUS). As a result, this serotype is routinely screened for in clinical microbiology laboratories. With the bias toward the identification of the O157 serogroup in routine diagnostic processes, non-O157 STEC has been largely underrepresented in the epidemiology of STEC infections. This diagnostic bias is further complicated by the fact that many non-O157 STEC infections cause nonspecific gastroenteritis symptoms reminiscent of enteric viral infections. In this study, real-time PCR was used to amplify Shiga toxin genetic determinants ( stx 1 and stx 2 ) from enriched stool samples that were initially submitted for the testing of enteric viruses in patients with suspected viral gastroenteritis between May and September of 2006, 2007, and 2008 ( n = 2,702). Samples were submitted from the province of Alberta, Yukon, the Northwest Territories, and Nunavut, Canada. A total of 38 samples (1.4%) tested positive for Shiga toxin genes, and 15 isolates were cultured for further characterization. Several of the serotypes identified (O157:H7, O26:HNM, O26:H11, O103:H25, O121:H19, and O145:HNM) have been previously associated with outbreaks and HUS. This study outlines the importance of combining molecular methods with classical culture techniques to enhance the detection of emerging non-O157 as well as O157 serotypes in diarrheal stool samples. Furthermore, atypical diarrhea disease caused by non-O157 STEC can be routinely missed due to screening only for viral agents. |
format |
Article in Journal/Newspaper |
author |
Couturier, Marc Roger Lee, Bonita Zelyas, Nathan Chui, Linda |
spellingShingle |
Couturier, Marc Roger Lee, Bonita Zelyas, Nathan Chui, Linda Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada |
author_facet |
Couturier, Marc Roger Lee, Bonita Zelyas, Nathan Chui, Linda |
author_sort |
Couturier, Marc Roger |
title |
Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada |
title_short |
Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada |
title_full |
Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada |
title_fullStr |
Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada |
title_full_unstemmed |
Shiga-Toxigenic Escherichia coli Detection in Stool Samples Screened for Viral Gastroenteritis in Alberta, Canada |
title_sort |
shiga-toxigenic escherichia coli detection in stool samples screened for viral gastroenteritis in alberta, canada |
publisher |
American Society for Microbiology |
publishDate |
2011 |
url |
http://dx.doi.org/10.1128/jcm.01693-10 https://journals.asm.org/doi/pdf/10.1128/JCM.01693-10 |
genre |
Northwest Territories Nunavut Yukon |
genre_facet |
Northwest Territories Nunavut Yukon |
op_source |
Journal of Clinical Microbiology volume 49, issue 2, page 574-578 ISSN 0095-1137 1098-660X |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/jcm.01693-10 |
container_title |
Journal of Clinical Microbiology |
container_volume |
49 |
container_issue |
2 |
container_start_page |
574 |
op_container_end_page |
578 |
_version_ |
1810467167877988352 |