Relationship of Critical Temperature to Macromolecular Synthesis and Growth Yield in Psychrobacter cryopegella
ABSTRACT Most microorganisms isolated from low-temperature environments (below 4°C) are eury-, not steno-, psychrophiles. While psychrophiles maximize or maintain growth yield at low temperatures to compensate for low growth rate, the mechanisms involved remain unknown, as does the strategy used by...
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Online Access: | http://dx.doi.org/10.1128/jb.186.8.2340-2345.2004 https://journals.asm.org/doi/pdf/10.1128/JB.186.8.2340-2345.2004 |
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crasmicro:10.1128/jb.186.8.2340-2345.2004 2024-09-15T18:30:09+00:00 Relationship of Critical Temperature to Macromolecular Synthesis and Growth Yield in Psychrobacter cryopegella Bakermans, Corien Nealson, Kenneth H. 2004 http://dx.doi.org/10.1128/jb.186.8.2340-2345.2004 https://journals.asm.org/doi/pdf/10.1128/JB.186.8.2340-2345.2004 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Journal of Bacteriology volume 186, issue 8, page 2340-2345 ISSN 0021-9193 1098-5530 journal-article 2004 crasmicro https://doi.org/10.1128/jb.186.8.2340-2345.2004 2024-08-12T04:06:35Z ABSTRACT Most microorganisms isolated from low-temperature environments (below 4°C) are eury-, not steno-, psychrophiles. While psychrophiles maximize or maintain growth yield at low temperatures to compensate for low growth rate, the mechanisms involved remain unknown, as does the strategy used by eurypsychrophiles to survive wide ranges of temperatures that include subzero temperatures. Our studies involve the eurypsychrophilic bacterium Psychrobacter cryopegella , which was isolated from a briny water lens within Siberian permafrost, where the temperature is −12°C. P. cryopegella is capable of reproducing from −10 to 28°C, with its maximum growth rate at 22°C. We examined the temperature dependence of growth rate, growth yield, and macromolecular (DNA, RNA, and protein) synthesis rates for P. cryopegella. Below 22°C, the growth of P. cryopegella was separated into two domains at the critical temperature ( T critical = 4°C). RNA, protein, and DNA synthesis rates decreased exponentially with decreasing temperatures. Only the temperature dependence of the DNA synthesis rate changed at T critical . When normalized to growth rate, RNA and protein synthesis reached a minimum at T critical , while DNA synthesis remained constant over the entire temperature range. Growth yield peaked at about T critical and declined rapidly as temperature decreased further. Similar to some stenopsychrophiles, P. cryopegella maximized growth yield at low temperatures and did so by streamlining growth processes at T critical . Identifying the specific processes which result in T critical will be vital to understanding both low-temperature growth and growth over a wide range of temperatures. Article in Journal/Newspaper permafrost ASM Journals (American Society for Microbiology) Journal of Bacteriology 186 8 2340 2345 |
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English |
description |
ABSTRACT Most microorganisms isolated from low-temperature environments (below 4°C) are eury-, not steno-, psychrophiles. While psychrophiles maximize or maintain growth yield at low temperatures to compensate for low growth rate, the mechanisms involved remain unknown, as does the strategy used by eurypsychrophiles to survive wide ranges of temperatures that include subzero temperatures. Our studies involve the eurypsychrophilic bacterium Psychrobacter cryopegella , which was isolated from a briny water lens within Siberian permafrost, where the temperature is −12°C. P. cryopegella is capable of reproducing from −10 to 28°C, with its maximum growth rate at 22°C. We examined the temperature dependence of growth rate, growth yield, and macromolecular (DNA, RNA, and protein) synthesis rates for P. cryopegella. Below 22°C, the growth of P. cryopegella was separated into two domains at the critical temperature ( T critical = 4°C). RNA, protein, and DNA synthesis rates decreased exponentially with decreasing temperatures. Only the temperature dependence of the DNA synthesis rate changed at T critical . When normalized to growth rate, RNA and protein synthesis reached a minimum at T critical , while DNA synthesis remained constant over the entire temperature range. Growth yield peaked at about T critical and declined rapidly as temperature decreased further. Similar to some stenopsychrophiles, P. cryopegella maximized growth yield at low temperatures and did so by streamlining growth processes at T critical . Identifying the specific processes which result in T critical will be vital to understanding both low-temperature growth and growth over a wide range of temperatures. |
format |
Article in Journal/Newspaper |
author |
Bakermans, Corien Nealson, Kenneth H. |
spellingShingle |
Bakermans, Corien Nealson, Kenneth H. Relationship of Critical Temperature to Macromolecular Synthesis and Growth Yield in Psychrobacter cryopegella |
author_facet |
Bakermans, Corien Nealson, Kenneth H. |
author_sort |
Bakermans, Corien |
title |
Relationship of Critical Temperature to Macromolecular Synthesis and Growth Yield in Psychrobacter cryopegella |
title_short |
Relationship of Critical Temperature to Macromolecular Synthesis and Growth Yield in Psychrobacter cryopegella |
title_full |
Relationship of Critical Temperature to Macromolecular Synthesis and Growth Yield in Psychrobacter cryopegella |
title_fullStr |
Relationship of Critical Temperature to Macromolecular Synthesis and Growth Yield in Psychrobacter cryopegella |
title_full_unstemmed |
Relationship of Critical Temperature to Macromolecular Synthesis and Growth Yield in Psychrobacter cryopegella |
title_sort |
relationship of critical temperature to macromolecular synthesis and growth yield in psychrobacter cryopegella |
publisher |
American Society for Microbiology |
publishDate |
2004 |
url |
http://dx.doi.org/10.1128/jb.186.8.2340-2345.2004 https://journals.asm.org/doi/pdf/10.1128/JB.186.8.2340-2345.2004 |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
Journal of Bacteriology volume 186, issue 8, page 2340-2345 ISSN 0021-9193 1098-5530 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/jb.186.8.2340-2345.2004 |
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Journal of Bacteriology |
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186 |
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8 |
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2340 |
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2345 |
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1810471623323549696 |