Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa
ABSTRACT A genomic island consisting of 14 open reading frames, orfA to orfN was previously identified in Pseudomonas aeruginosa strain PAK and shown to be essential for glycosylation of flagellin. DNA microarray hybridization analysis of a number of P. aeruginosa strains from diverse origins showed...
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crasmicro:10.1128/jb.186.7.2115-2122.2004 2024-04-14T08:19:18+00:00 Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa Arora, Shiwani K. Wolfgang, Matthew C. Lory, Stephen Ramphal, Reuben 2004 http://dx.doi.org/10.1128/jb.186.7.2115-2122.2004 https://journals.asm.org/doi/pdf/10.1128/JB.186.7.2115-2122.2004 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Journal of Bacteriology volume 186, issue 7, page 2115-2122 ISSN 0021-9193 1098-5530 Molecular Biology Microbiology journal-article 2004 crasmicro https://doi.org/10.1128/jb.186.7.2115-2122.2004 2024-03-15T07:50:46Z ABSTRACT A genomic island consisting of 14 open reading frames, orfA to orfN was previously identified in Pseudomonas aeruginosa strain PAK and shown to be essential for glycosylation of flagellin. DNA microarray hybridization analysis of a number of P. aeruginosa strains from diverse origins showed that this island is polymorphic. PCR and sequence analysis confirmed that many P. aeruginosa strains carry an abbreviated version of the island (short island) in which orfD , - E and - H are polymorphic and orfI , - J , - K , - L , and - M are absent. To ascertain whether there was a relationship between the inheritance of the short island and specific flagellin sequence variants, complete or partial nucleotide sequences of flagellin genes from 24 a-type P. aeruginosa strains were determined. Two distinct flagellin subtypes, designated A1 and A2, were apparent. Strains with the complete 14-gene island (long island) were almost exclusively of the A1 type, whereas strains carrying the short island were associated with both A1- and A2-type flagellins. These findings indicate that P. aeruginosa possesses a relatively low number of distinct flagellin types and probably has the capacity to further diversify this antigenic surface protein by glycosylation. Article in Journal/Newspaper Short Island ASM Journals (American Society for Microbiology) Long Island Short Island ENVELOPE(-60.400,-60.400,-63.950,-63.950) Journal of Bacteriology 186 7 2115 2122 |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
topic |
Molecular Biology Microbiology |
spellingShingle |
Molecular Biology Microbiology Arora, Shiwani K. Wolfgang, Matthew C. Lory, Stephen Ramphal, Reuben Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa |
topic_facet |
Molecular Biology Microbiology |
description |
ABSTRACT A genomic island consisting of 14 open reading frames, orfA to orfN was previously identified in Pseudomonas aeruginosa strain PAK and shown to be essential for glycosylation of flagellin. DNA microarray hybridization analysis of a number of P. aeruginosa strains from diverse origins showed that this island is polymorphic. PCR and sequence analysis confirmed that many P. aeruginosa strains carry an abbreviated version of the island (short island) in which orfD , - E and - H are polymorphic and orfI , - J , - K , - L , and - M are absent. To ascertain whether there was a relationship between the inheritance of the short island and specific flagellin sequence variants, complete or partial nucleotide sequences of flagellin genes from 24 a-type P. aeruginosa strains were determined. Two distinct flagellin subtypes, designated A1 and A2, were apparent. Strains with the complete 14-gene island (long island) were almost exclusively of the A1 type, whereas strains carrying the short island were associated with both A1- and A2-type flagellins. These findings indicate that P. aeruginosa possesses a relatively low number of distinct flagellin types and probably has the capacity to further diversify this antigenic surface protein by glycosylation. |
format |
Article in Journal/Newspaper |
author |
Arora, Shiwani K. Wolfgang, Matthew C. Lory, Stephen Ramphal, Reuben |
author_facet |
Arora, Shiwani K. Wolfgang, Matthew C. Lory, Stephen Ramphal, Reuben |
author_sort |
Arora, Shiwani K. |
title |
Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa |
title_short |
Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa |
title_full |
Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa |
title_fullStr |
Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa |
title_full_unstemmed |
Sequence Polymorphism in the Glycosylation Island and Flagellins of Pseudomonas aeruginosa |
title_sort |
sequence polymorphism in the glycosylation island and flagellins of pseudomonas aeruginosa |
publisher |
American Society for Microbiology |
publishDate |
2004 |
url |
http://dx.doi.org/10.1128/jb.186.7.2115-2122.2004 https://journals.asm.org/doi/pdf/10.1128/JB.186.7.2115-2122.2004 |
long_lat |
ENVELOPE(-60.400,-60.400,-63.950,-63.950) |
geographic |
Long Island Short Island |
geographic_facet |
Long Island Short Island |
genre |
Short Island |
genre_facet |
Short Island |
op_source |
Journal of Bacteriology volume 186, issue 7, page 2115-2122 ISSN 0021-9193 1098-5530 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/jb.186.7.2115-2122.2004 |
container_title |
Journal of Bacteriology |
container_volume |
186 |
container_issue |
7 |
container_start_page |
2115 |
op_container_end_page |
2122 |
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1796318975793037312 |