Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation
ABSTRACT Direct analysis of membrane lipids by liquid chromatography-electrospray mass spectrometry was used to demonstrate the role of unsaturation in ether lipids in the adaptation of Methanococcoides burtonii to low temperature. A proteomics approach using two-dimensional liquid chromatography-ma...
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Online Access: | http://dx.doi.org/10.1128/jb.186.24.8508-8515.2004 https://journals.asm.org/doi/pdf/10.1128/JB.186.24.8508-8515.2004 |
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crasmicro:10.1128/jb.186.24.8508-8515.2004 2024-09-15T17:48:13+00:00 Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation Nichols, David S. Miller, Matthew R. Davies, Noel W. Goodchild, Amber Raftery, Mark Cavicchioli, Ricardo 2004 http://dx.doi.org/10.1128/jb.186.24.8508-8515.2004 https://journals.asm.org/doi/pdf/10.1128/JB.186.24.8508-8515.2004 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Journal of Bacteriology volume 186, issue 24, page 8508-8515 ISSN 0021-9193 1098-5530 journal-article 2004 crasmicro https://doi.org/10.1128/jb.186.24.8508-8515.2004 2024-08-05T04:10:10Z ABSTRACT Direct analysis of membrane lipids by liquid chromatography-electrospray mass spectrometry was used to demonstrate the role of unsaturation in ether lipids in the adaptation of Methanococcoides burtonii to low temperature. A proteomics approach using two-dimensional liquid chromatography-mass spectrometry was used to identify enzymes involved in lipid biosynthesis, and a pathway for lipid biosynthesis was reconstructed from the M. burtonii draft genome sequence. The major phospholipids were archaeol phosphatidylglycerol, archaeol phosphatidylinositol, hydroxyarchaeol phosphatidylglycerol, and hydroxyarchaeol phosphatidylinositol. All phospholipid classes contained a series of unsaturated analogues, with the degree of unsaturation dependent on phospholipid class. The proportion of unsaturated lipids from cells grown at 4°C was significantly higher than for cells grown at 23°C. 3-Hydroxy-3-methylglutaryl coenzyme A synthase, farnesyl diphosphate synthase, and geranylgeranyl diphosphate synthase were identified in the expressed proteome, and most genes involved in the mevalonate pathway and processes leading to the formation of phosphatidylinositol and phosphatidylglycerol were identified in the genome sequence. In addition, M. burtonii encodes CDP-inositol and CDP-glycerol transferases and a number of homologs of the plant geranylgeranyl reductase. It therefore appears that the unsaturation of lipids may be due to incomplete reduction of an archaeol precursor rather than to a desaturase mechanism. This study shows that cold adaptation in M. burtonii involves specific changes in membrane lipid unsaturation. It also demonstrates that global methods of analysis for lipids and proteomics linked to a draft genome sequence can be effectively combined to infer specific mechanisms of key biological processes. Article in Journal/Newspaper Antarc* Antarctic ASM Journals (American Society for Microbiology) Journal of Bacteriology 186 24 8508 8515 |
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ASM Journals (American Society for Microbiology) |
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English |
description |
ABSTRACT Direct analysis of membrane lipids by liquid chromatography-electrospray mass spectrometry was used to demonstrate the role of unsaturation in ether lipids in the adaptation of Methanococcoides burtonii to low temperature. A proteomics approach using two-dimensional liquid chromatography-mass spectrometry was used to identify enzymes involved in lipid biosynthesis, and a pathway for lipid biosynthesis was reconstructed from the M. burtonii draft genome sequence. The major phospholipids were archaeol phosphatidylglycerol, archaeol phosphatidylinositol, hydroxyarchaeol phosphatidylglycerol, and hydroxyarchaeol phosphatidylinositol. All phospholipid classes contained a series of unsaturated analogues, with the degree of unsaturation dependent on phospholipid class. The proportion of unsaturated lipids from cells grown at 4°C was significantly higher than for cells grown at 23°C. 3-Hydroxy-3-methylglutaryl coenzyme A synthase, farnesyl diphosphate synthase, and geranylgeranyl diphosphate synthase were identified in the expressed proteome, and most genes involved in the mevalonate pathway and processes leading to the formation of phosphatidylinositol and phosphatidylglycerol were identified in the genome sequence. In addition, M. burtonii encodes CDP-inositol and CDP-glycerol transferases and a number of homologs of the plant geranylgeranyl reductase. It therefore appears that the unsaturation of lipids may be due to incomplete reduction of an archaeol precursor rather than to a desaturase mechanism. This study shows that cold adaptation in M. burtonii involves specific changes in membrane lipid unsaturation. It also demonstrates that global methods of analysis for lipids and proteomics linked to a draft genome sequence can be effectively combined to infer specific mechanisms of key biological processes. |
format |
Article in Journal/Newspaper |
author |
Nichols, David S. Miller, Matthew R. Davies, Noel W. Goodchild, Amber Raftery, Mark Cavicchioli, Ricardo |
spellingShingle |
Nichols, David S. Miller, Matthew R. Davies, Noel W. Goodchild, Amber Raftery, Mark Cavicchioli, Ricardo Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation |
author_facet |
Nichols, David S. Miller, Matthew R. Davies, Noel W. Goodchild, Amber Raftery, Mark Cavicchioli, Ricardo |
author_sort |
Nichols, David S. |
title |
Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation |
title_short |
Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation |
title_full |
Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation |
title_fullStr |
Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation |
title_full_unstemmed |
Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii Involves Membrane Lipid Unsaturation |
title_sort |
cold adaptation in the antarctic archaeon methanococcoides burtonii involves membrane lipid unsaturation |
publisher |
American Society for Microbiology |
publishDate |
2004 |
url |
http://dx.doi.org/10.1128/jb.186.24.8508-8515.2004 https://journals.asm.org/doi/pdf/10.1128/JB.186.24.8508-8515.2004 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Journal of Bacteriology volume 186, issue 24, page 8508-8515 ISSN 0021-9193 1098-5530 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/jb.186.24.8508-8515.2004 |
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Journal of Bacteriology |
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186 |
container_issue |
24 |
container_start_page |
8508 |
op_container_end_page |
8515 |
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1810289383183482880 |