Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System
ABSTRACT A newly characterized archaeal rudivirus Stygiolobus rod-shaped virus (SRV), which infects a hyperthermophilic Stygiolobus species, was isolated from a hot spring in the Azores, Portugal. Its virions are rod-shaped, 702 (± 50) by 22 (± 3) nm in size, and nonenveloped and carry three tail fi...
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crasmicro:10.1128/jb.00795-08 2024-06-23T07:54:05+00:00 Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System Vestergaard, Gisle Shah, Shiraz A. Bize, Ariane Reitberger, Werner Reuter, Monika Phan, Hien Briegel, Ariane Rachel, Reinhard Garrett, Roger A. Prangishvili, David 2008 http://dx.doi.org/10.1128/jb.00795-08 https://journals.asm.org/doi/pdf/10.1128/JB.00795-08 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Journal of Bacteriology volume 190, issue 20, page 6837-6845 ISSN 0021-9193 1098-5530 journal-article 2008 crasmicro https://doi.org/10.1128/jb.00795-08 2024-06-10T04:07:10Z ABSTRACT A newly characterized archaeal rudivirus Stygiolobus rod-shaped virus (SRV), which infects a hyperthermophilic Stygiolobus species, was isolated from a hot spring in the Azores, Portugal. Its virions are rod-shaped, 702 (± 50) by 22 (± 3) nm in size, and nonenveloped and carry three tail fibers at each terminus. The linear double-stranded DNA genome contains 28,096 bp and an inverted terminal repeat of 1,030 bp. The SRV shows morphological and genomic similarities to the other characterized rudiviruses Sulfolobus rod-shaped virus 1 (SIRV1), SIRV2, and Acidianus rod-shaped virus 1, isolated from hot acidic springs of Iceland and Italy. The single major rudiviral structural protein is shown to generate long tubular structures in vitro of similar dimensions to those of the virion, and we estimate that the virion constitutes a single, superhelical, double-stranded DNA embedded into such a protein structure. Three additional minor conserved structural proteins are also identified. Ubiquitous rudiviral proteins with assigned functions include glycosyl transferases and a S -adenosylmethionine-dependent methyltransferase, as well as a Holliday junction resolvase, a transcriptionally coupled helicase and nuclease implicated in DNA replication. Analysis of matches between known crenarchaeal chromosomal CRISPR spacer sequences, implicated in a viral defense system, and rudiviral genomes revealed that about 10% of the 3,042 unique acidothermophile spacers yield significant matches to rudiviral genomes, with a bias to highly conserved protein genes, consistent with the widespread presence of rudiviruses in hot acidophilic environments. We propose that the 12-bp indels which are commonly found in conserved rudiviral protein genes may be generated as a reaction to the presence of the host CRISPR defense system. Article in Journal/Newspaper Iceland ASM Journals (American Society for Microbiology) Journal of Bacteriology 190 20 6837 6845 |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT A newly characterized archaeal rudivirus Stygiolobus rod-shaped virus (SRV), which infects a hyperthermophilic Stygiolobus species, was isolated from a hot spring in the Azores, Portugal. Its virions are rod-shaped, 702 (± 50) by 22 (± 3) nm in size, and nonenveloped and carry three tail fibers at each terminus. The linear double-stranded DNA genome contains 28,096 bp and an inverted terminal repeat of 1,030 bp. The SRV shows morphological and genomic similarities to the other characterized rudiviruses Sulfolobus rod-shaped virus 1 (SIRV1), SIRV2, and Acidianus rod-shaped virus 1, isolated from hot acidic springs of Iceland and Italy. The single major rudiviral structural protein is shown to generate long tubular structures in vitro of similar dimensions to those of the virion, and we estimate that the virion constitutes a single, superhelical, double-stranded DNA embedded into such a protein structure. Three additional minor conserved structural proteins are also identified. Ubiquitous rudiviral proteins with assigned functions include glycosyl transferases and a S -adenosylmethionine-dependent methyltransferase, as well as a Holliday junction resolvase, a transcriptionally coupled helicase and nuclease implicated in DNA replication. Analysis of matches between known crenarchaeal chromosomal CRISPR spacer sequences, implicated in a viral defense system, and rudiviral genomes revealed that about 10% of the 3,042 unique acidothermophile spacers yield significant matches to rudiviral genomes, with a bias to highly conserved protein genes, consistent with the widespread presence of rudiviruses in hot acidophilic environments. We propose that the 12-bp indels which are commonly found in conserved rudiviral protein genes may be generated as a reaction to the presence of the host CRISPR defense system. |
format |
Article in Journal/Newspaper |
author |
Vestergaard, Gisle Shah, Shiraz A. Bize, Ariane Reitberger, Werner Reuter, Monika Phan, Hien Briegel, Ariane Rachel, Reinhard Garrett, Roger A. Prangishvili, David |
spellingShingle |
Vestergaard, Gisle Shah, Shiraz A. Bize, Ariane Reitberger, Werner Reuter, Monika Phan, Hien Briegel, Ariane Rachel, Reinhard Garrett, Roger A. Prangishvili, David Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System |
author_facet |
Vestergaard, Gisle Shah, Shiraz A. Bize, Ariane Reitberger, Werner Reuter, Monika Phan, Hien Briegel, Ariane Rachel, Reinhard Garrett, Roger A. Prangishvili, David |
author_sort |
Vestergaard, Gisle |
title |
Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System |
title_short |
Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System |
title_full |
Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System |
title_fullStr |
Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System |
title_full_unstemmed |
Stygiolobus Rod-Shaped Virus and the Interplay of Crenarchaeal Rudiviruses with the CRISPR Antiviral System |
title_sort |
stygiolobus rod-shaped virus and the interplay of crenarchaeal rudiviruses with the crispr antiviral system |
publisher |
American Society for Microbiology |
publishDate |
2008 |
url |
http://dx.doi.org/10.1128/jb.00795-08 https://journals.asm.org/doi/pdf/10.1128/JB.00795-08 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Journal of Bacteriology volume 190, issue 20, page 6837-6845 ISSN 0021-9193 1098-5530 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/jb.00795-08 |
container_title |
Journal of Bacteriology |
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190 |
container_issue |
20 |
container_start_page |
6837 |
op_container_end_page |
6845 |
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1802646043280539648 |