An aromatic-dependent mutant of the fish pathogen Aeromonas salmonicida is attenuated in fish and is effective as a live vaccine against the salmonid disease furunculosis
Aeromonas salmonicida is the etiological agent of furunculosis in salmonid fish. The disease is responsible for severe economic losses in intensively cultured salmon and trout. Bacterin vaccines provide inadequate protection against infection. We have constructed an aromatic-dependent mutant of A. s...
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American Society for Microbiology
1993
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Online Access: | http://dx.doi.org/10.1128/iai.61.5.2172-2181.1993 https://journals.asm.org/doi/pdf/10.1128/iai.61.5.2172-2181.1993 |
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crasmicro:10.1128/iai.61.5.2172-2181.1993 2023-11-05T03:40:35+01:00 An aromatic-dependent mutant of the fish pathogen Aeromonas salmonicida is attenuated in fish and is effective as a live vaccine against the salmonid disease furunculosis Vaughan, L M Smith, P R Foster, T J 1993 http://dx.doi.org/10.1128/iai.61.5.2172-2181.1993 https://journals.asm.org/doi/pdf/10.1128/iai.61.5.2172-2181.1993 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Infection and Immunity volume 61, issue 5, page 2172-2181 ISSN 0019-9567 1098-5522 Infectious Diseases Immunology Microbiology Parasitology journal-article 1993 crasmicro https://doi.org/10.1128/iai.61.5.2172-2181.1993 2023-10-09T16:09:55Z Aeromonas salmonicida is the etiological agent of furunculosis in salmonid fish. The disease is responsible for severe economic losses in intensively cultured salmon and trout. Bacterin vaccines provide inadequate protection against infection. We have constructed an aromatic-dependent mutant of A. salmonicida in order to investigate the possibility of an effective live-attenuated vaccine. The aroA gene of A. salmonicida was cloned in Escherichia coli, and the nucleotide sequence was determined. The codon usage pattern of aroA was found to be quite distinct from that of the vapA gene coding for the surface array protein layer (A layer). The aroA gene was inactivated by inserting a fragment expressing kanamycin resistance within the coding sequence. The aroA::Kar mutation was introduced into the chromosome of virulent A. salmonicida 644Rb and 640V2 by allele replacement by using a suicide plasmid delivery system. The aroA mutation did not revert at a detectable frequency (< 10(-11). The mutation resulted in attenuation when bacteria were injected intramuscularly into Atlantic salmon (Salmo salar L.). Introduction of the wild-type aroA gene into the A. salmonicida mutants on a broad-host-range plasmid restored virulence. A. salmonicida mutant 644Rb aroA::Kar persisted in the kidney of brown trout (Salmo trutta L.) for 12 days at 10 degrees C. Vaccination of brown trout with 10(7) CFU of A. salmonicida 644Rb aroA by intraperitoneal injection resulted in a 253-fold increase in the 50% lethal dose (LD50) compared with unvaccinated controls challenged with a virulent clinical isolate 9 weeks later. A second vaccination after 6 weeks increased the LD50 by a further 16-fold. Article in Journal/Newspaper Atlantic salmon Salmo salar ASM Journals (American Society for Microbiology - via Crossref) Infection and Immunity 61 5 2172 2181 |
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Open Polar |
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ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Infectious Diseases Immunology Microbiology Parasitology |
spellingShingle |
Infectious Diseases Immunology Microbiology Parasitology Vaughan, L M Smith, P R Foster, T J An aromatic-dependent mutant of the fish pathogen Aeromonas salmonicida is attenuated in fish and is effective as a live vaccine against the salmonid disease furunculosis |
topic_facet |
Infectious Diseases Immunology Microbiology Parasitology |
description |
Aeromonas salmonicida is the etiological agent of furunculosis in salmonid fish. The disease is responsible for severe economic losses in intensively cultured salmon and trout. Bacterin vaccines provide inadequate protection against infection. We have constructed an aromatic-dependent mutant of A. salmonicida in order to investigate the possibility of an effective live-attenuated vaccine. The aroA gene of A. salmonicida was cloned in Escherichia coli, and the nucleotide sequence was determined. The codon usage pattern of aroA was found to be quite distinct from that of the vapA gene coding for the surface array protein layer (A layer). The aroA gene was inactivated by inserting a fragment expressing kanamycin resistance within the coding sequence. The aroA::Kar mutation was introduced into the chromosome of virulent A. salmonicida 644Rb and 640V2 by allele replacement by using a suicide plasmid delivery system. The aroA mutation did not revert at a detectable frequency (< 10(-11). The mutation resulted in attenuation when bacteria were injected intramuscularly into Atlantic salmon (Salmo salar L.). Introduction of the wild-type aroA gene into the A. salmonicida mutants on a broad-host-range plasmid restored virulence. A. salmonicida mutant 644Rb aroA::Kar persisted in the kidney of brown trout (Salmo trutta L.) for 12 days at 10 degrees C. Vaccination of brown trout with 10(7) CFU of A. salmonicida 644Rb aroA by intraperitoneal injection resulted in a 253-fold increase in the 50% lethal dose (LD50) compared with unvaccinated controls challenged with a virulent clinical isolate 9 weeks later. A second vaccination after 6 weeks increased the LD50 by a further 16-fold. |
format |
Article in Journal/Newspaper |
author |
Vaughan, L M Smith, P R Foster, T J |
author_facet |
Vaughan, L M Smith, P R Foster, T J |
author_sort |
Vaughan, L M |
title |
An aromatic-dependent mutant of the fish pathogen Aeromonas salmonicida is attenuated in fish and is effective as a live vaccine against the salmonid disease furunculosis |
title_short |
An aromatic-dependent mutant of the fish pathogen Aeromonas salmonicida is attenuated in fish and is effective as a live vaccine against the salmonid disease furunculosis |
title_full |
An aromatic-dependent mutant of the fish pathogen Aeromonas salmonicida is attenuated in fish and is effective as a live vaccine against the salmonid disease furunculosis |
title_fullStr |
An aromatic-dependent mutant of the fish pathogen Aeromonas salmonicida is attenuated in fish and is effective as a live vaccine against the salmonid disease furunculosis |
title_full_unstemmed |
An aromatic-dependent mutant of the fish pathogen Aeromonas salmonicida is attenuated in fish and is effective as a live vaccine against the salmonid disease furunculosis |
title_sort |
aromatic-dependent mutant of the fish pathogen aeromonas salmonicida is attenuated in fish and is effective as a live vaccine against the salmonid disease furunculosis |
publisher |
American Society for Microbiology |
publishDate |
1993 |
url |
http://dx.doi.org/10.1128/iai.61.5.2172-2181.1993 https://journals.asm.org/doi/pdf/10.1128/iai.61.5.2172-2181.1993 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Infection and Immunity volume 61, issue 5, page 2172-2181 ISSN 0019-9567 1098-5522 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/iai.61.5.2172-2181.1993 |
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Infection and Immunity |
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61 |
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5 |
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2172 |
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2181 |
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1781696722760630272 |