Class and subclass antibodies to Haemophilus influenzae type b capsule: comparison of invasive disease and natural exposure
Recently, the role of immunoglobulin G (IgG) subclasses in the immune responses to organisms with polysaccharide capsules, particularly Haemophilus influenzae type b, has been of interest. We developed assays to measure IgG2- and IgG4-specific antibodies to the polyribosylribitol phosphate (PRP) pol...
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American Society for Microbiology
1986
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Online Access: | http://dx.doi.org/10.1128/iai.53.3.486-490.1986 https://journals.asm.org/doi/pdf/10.1128/iai.53.3.486-490.1986 |
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crasmicro:10.1128/iai.53.3.486-490.1986 2023-05-15T16:07:53+02:00 Class and subclass antibodies to Haemophilus influenzae type b capsule: comparison of invasive disease and natural exposure Ramadas, K Petersen, G M Heiner, D C Ward, J I 1986 http://dx.doi.org/10.1128/iai.53.3.486-490.1986 https://journals.asm.org/doi/pdf/10.1128/iai.53.3.486-490.1986 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Infection and Immunity volume 53, issue 3, page 486-490 ISSN 0019-9567 1098-5522 Infectious Diseases Immunology Microbiology Parasitology journal-article 1986 crasmicro https://doi.org/10.1128/iai.53.3.486-490.1986 2022-10-30T09:12:34Z Recently, the role of immunoglobulin G (IgG) subclasses in the immune responses to organisms with polysaccharide capsules, particularly Haemophilus influenzae type b, has been of interest. We developed assays to measure IgG2- and IgG4-specific antibodies to the polyribosylribitol phosphate (PRP) polysaccharide antigen of H. influenzae type b and demonstrated that these assays were subclass specific. Relative levels of subclass-specific antibody were assayed in serum from 30 Alaskan Eskimo children who had invasive H. influenzae type b disease and 30 healthy controls that were matched for age and village of residence. We also measured total PRP antibody and total serum IgG4. The group with invasive H. influenzae type b disease had a significantly higher mean level of IgG4-specific PRP antibody than did the controls (P = 0.0006). However, we found no significant difference between cases and controls for IgG2-specific PRP antibody, total IgG4, or total PRP antibody. The data suggest that IgG4-specific PRP antibody is elicited by invasive H. influenzae type b disease, independent of age. The IgG4 subclass thus may be a critical determinant of the immune response to invasive infection caused by H. influenzae type b, especially for young infants who generally have a weak immune response to this organism. Article in Journal/Newspaper eskimo* ASM Journals (American Society for Microbiology - via Crossref) Infection and Immunity 53 3 486 490 |
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Open Polar |
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ASM Journals (American Society for Microbiology - via Crossref) |
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crasmicro |
language |
English |
topic |
Infectious Diseases Immunology Microbiology Parasitology |
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Infectious Diseases Immunology Microbiology Parasitology Ramadas, K Petersen, G M Heiner, D C Ward, J I Class and subclass antibodies to Haemophilus influenzae type b capsule: comparison of invasive disease and natural exposure |
topic_facet |
Infectious Diseases Immunology Microbiology Parasitology |
description |
Recently, the role of immunoglobulin G (IgG) subclasses in the immune responses to organisms with polysaccharide capsules, particularly Haemophilus influenzae type b, has been of interest. We developed assays to measure IgG2- and IgG4-specific antibodies to the polyribosylribitol phosphate (PRP) polysaccharide antigen of H. influenzae type b and demonstrated that these assays were subclass specific. Relative levels of subclass-specific antibody were assayed in serum from 30 Alaskan Eskimo children who had invasive H. influenzae type b disease and 30 healthy controls that were matched for age and village of residence. We also measured total PRP antibody and total serum IgG4. The group with invasive H. influenzae type b disease had a significantly higher mean level of IgG4-specific PRP antibody than did the controls (P = 0.0006). However, we found no significant difference between cases and controls for IgG2-specific PRP antibody, total IgG4, or total PRP antibody. The data suggest that IgG4-specific PRP antibody is elicited by invasive H. influenzae type b disease, independent of age. The IgG4 subclass thus may be a critical determinant of the immune response to invasive infection caused by H. influenzae type b, especially for young infants who generally have a weak immune response to this organism. |
format |
Article in Journal/Newspaper |
author |
Ramadas, K Petersen, G M Heiner, D C Ward, J I |
author_facet |
Ramadas, K Petersen, G M Heiner, D C Ward, J I |
author_sort |
Ramadas, K |
title |
Class and subclass antibodies to Haemophilus influenzae type b capsule: comparison of invasive disease and natural exposure |
title_short |
Class and subclass antibodies to Haemophilus influenzae type b capsule: comparison of invasive disease and natural exposure |
title_full |
Class and subclass antibodies to Haemophilus influenzae type b capsule: comparison of invasive disease and natural exposure |
title_fullStr |
Class and subclass antibodies to Haemophilus influenzae type b capsule: comparison of invasive disease and natural exposure |
title_full_unstemmed |
Class and subclass antibodies to Haemophilus influenzae type b capsule: comparison of invasive disease and natural exposure |
title_sort |
class and subclass antibodies to haemophilus influenzae type b capsule: comparison of invasive disease and natural exposure |
publisher |
American Society for Microbiology |
publishDate |
1986 |
url |
http://dx.doi.org/10.1128/iai.53.3.486-490.1986 https://journals.asm.org/doi/pdf/10.1128/iai.53.3.486-490.1986 |
genre |
eskimo* |
genre_facet |
eskimo* |
op_source |
Infection and Immunity volume 53, issue 3, page 486-490 ISSN 0019-9567 1098-5522 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/iai.53.3.486-490.1986 |
container_title |
Infection and Immunity |
container_volume |
53 |
container_issue |
3 |
container_start_page |
486 |
op_container_end_page |
490 |
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1766403935151587328 |