Experimental Yersinia pestis infection in rodents after intragastric inoculation and ingestion of bacteria
To clarify the pathogenesis of oral plague infection, we studied the susceptibility of three species of rodents to intragastric inoculation of Yersinia pestis, described the pathology and progression of infection, and measured antibody responses to fraction IA antigen of Y. pestis. The 50% lethal do...
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American Society for Microbiology
1982
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Online Access: | http://dx.doi.org/10.1128/iai.36.3.1160-1167.1982 https://journals.asm.org/doi/pdf/10.1128/iai.36.3.1160-1167.1982 |
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crasmicro:10.1128/iai.36.3.1160-1167.1982 2023-10-09T21:55:33+02:00 Experimental Yersinia pestis infection in rodents after intragastric inoculation and ingestion of bacteria Butler, T Fu, Y S Furman, L Almeida, C Almeida, A 1982 http://dx.doi.org/10.1128/iai.36.3.1160-1167.1982 https://journals.asm.org/doi/pdf/10.1128/iai.36.3.1160-1167.1982 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Infection and Immunity volume 36, issue 3, page 1160-1167 ISSN 0019-9567 1098-5522 Infectious Diseases Immunology Microbiology Parasitology journal-article 1982 crasmicro https://doi.org/10.1128/iai.36.3.1160-1167.1982 2023-09-15T05:46:44Z To clarify the pathogenesis of oral plague infection, we studied the susceptibility of three species of rodents to intragastric inoculation of Yersinia pestis, described the pathology and progression of infection, and measured antibody responses to fraction IA antigen of Y. pestis. The 50% lethal doses of bacteria by intragastric inoculation for Mus musculus, Zygodontomys pixuna, and Rattus rattus were log10 = 6.32, 5.46, and 9.62, respectively, which were at least 1,000-fold higher than the values obtained by subcutaneous inoculation. M. musculus was shown to be susceptible to lethal infection also when bacteria were ingested in drinking water. Microscopic pathology was consistent with heavy systemic infection. Quantitative cultures of tissues at different times after intragastric inoculation revealed that infections of blood, liver, and spleen preceded infections of Peyer's patches and mesenteric lymph nodes. Stool cultures were negative. The strain of Y. pestis used for inoculation was killed when exposed to a buffered solution at pH less than or equal to 3. Antibody responses were observed in some of the surviving rodents after intragastric challenge. These results showed that Y. pestis was an effective oral pathogen that produced fatal systemic infections and self-limited infections with immunity but did not produce enteric pathology or lead to fecal excretion of bacteria. Article in Journal/Newspaper Rattus rattus ASM Journals (American Society for Microbiology - via Crossref) Infection and Immunity 36 3 1160 1167 |
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ASM Journals (American Society for Microbiology - via Crossref) |
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language |
English |
topic |
Infectious Diseases Immunology Microbiology Parasitology |
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Infectious Diseases Immunology Microbiology Parasitology Butler, T Fu, Y S Furman, L Almeida, C Almeida, A Experimental Yersinia pestis infection in rodents after intragastric inoculation and ingestion of bacteria |
topic_facet |
Infectious Diseases Immunology Microbiology Parasitology |
description |
To clarify the pathogenesis of oral plague infection, we studied the susceptibility of three species of rodents to intragastric inoculation of Yersinia pestis, described the pathology and progression of infection, and measured antibody responses to fraction IA antigen of Y. pestis. The 50% lethal doses of bacteria by intragastric inoculation for Mus musculus, Zygodontomys pixuna, and Rattus rattus were log10 = 6.32, 5.46, and 9.62, respectively, which were at least 1,000-fold higher than the values obtained by subcutaneous inoculation. M. musculus was shown to be susceptible to lethal infection also when bacteria were ingested in drinking water. Microscopic pathology was consistent with heavy systemic infection. Quantitative cultures of tissues at different times after intragastric inoculation revealed that infections of blood, liver, and spleen preceded infections of Peyer's patches and mesenteric lymph nodes. Stool cultures were negative. The strain of Y. pestis used for inoculation was killed when exposed to a buffered solution at pH less than or equal to 3. Antibody responses were observed in some of the surviving rodents after intragastric challenge. These results showed that Y. pestis was an effective oral pathogen that produced fatal systemic infections and self-limited infections with immunity but did not produce enteric pathology or lead to fecal excretion of bacteria. |
format |
Article in Journal/Newspaper |
author |
Butler, T Fu, Y S Furman, L Almeida, C Almeida, A |
author_facet |
Butler, T Fu, Y S Furman, L Almeida, C Almeida, A |
author_sort |
Butler, T |
title |
Experimental Yersinia pestis infection in rodents after intragastric inoculation and ingestion of bacteria |
title_short |
Experimental Yersinia pestis infection in rodents after intragastric inoculation and ingestion of bacteria |
title_full |
Experimental Yersinia pestis infection in rodents after intragastric inoculation and ingestion of bacteria |
title_fullStr |
Experimental Yersinia pestis infection in rodents after intragastric inoculation and ingestion of bacteria |
title_full_unstemmed |
Experimental Yersinia pestis infection in rodents after intragastric inoculation and ingestion of bacteria |
title_sort |
experimental yersinia pestis infection in rodents after intragastric inoculation and ingestion of bacteria |
publisher |
American Society for Microbiology |
publishDate |
1982 |
url |
http://dx.doi.org/10.1128/iai.36.3.1160-1167.1982 https://journals.asm.org/doi/pdf/10.1128/iai.36.3.1160-1167.1982 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Infection and Immunity volume 36, issue 3, page 1160-1167 ISSN 0019-9567 1098-5522 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/iai.36.3.1160-1167.1982 |
container_title |
Infection and Immunity |
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36 |
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3 |
container_start_page |
1160 |
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1167 |
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1779319504944234496 |