LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence
ABSTRACT Vibrio ( Aliivibrio ) salmonicida is the causal agent of cold-water vibriosis, a fatal bacterial septicemia primarily of farmed salmonid fish. The molecular mechanisms of invasion, colonization, and growth of V. salmonicida in the host are still largely unknown, and few virulence factors ha...
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Online Access: | http://dx.doi.org/10.1128/iai.06038-11 https://journals.asm.org/doi/pdf/10.1128/IAI.06038-11 |
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crasmicro:10.1128/iai.06038-11 2023-11-05T03:40:34+01:00 LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence Bjelland, Ane Mohn Sørum, Henning Tegegne, Daget Ayana Winther-Larsen, Hanne C. Willassen, Nils Peder Hansen, Hilde Camilli, A. 2012 http://dx.doi.org/10.1128/iai.06038-11 https://journals.asm.org/doi/pdf/10.1128/IAI.06038-11 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Infection and Immunity volume 80, issue 5, page 1681-1689 ISSN 0019-9567 1098-5522 Infectious Diseases Immunology Microbiology Parasitology journal-article 2012 crasmicro https://doi.org/10.1128/iai.06038-11 2023-10-09T16:11:19Z ABSTRACT Vibrio ( Aliivibrio ) salmonicida is the causal agent of cold-water vibriosis, a fatal bacterial septicemia primarily of farmed salmonid fish. The molecular mechanisms of invasion, colonization, and growth of V. salmonicida in the host are still largely unknown, and few virulence factors have been identified. Quorum sensing (QS) is a cell-to-cell communication system known to regulate virulence and other activities in several bacterial species. The genome of V. salmonicida LFI1238 encodes products presumably involved in several QS systems. In this study, the gene encoding LitR, a homolog of the master regulator of QS in V. fischeri , was deleted. Compared to the parental strain, the litR mutant showed increased motility, adhesion, cell-to-cell aggregation, and biofilm formation. Furthermore, the litR mutant produced less cryptic bioluminescence, whereas production of acylhomoserine lactones was unaffected. Our results also indicate a salinity-sensitive regulation of LitR. Finally, reduced mortality was observed in Atlantic salmon infected with the litR mutant, implying that the fish were more susceptible to infection with the wild type than with the mutant strain. We hypothesize that LitR inhibits biofilm formation and favors planktonic growth, with the latter being more adapted for pathogenesis in the fish host. Article in Journal/Newspaper Atlantic salmon ASM Journals (American Society for Microbiology - via Crossref) Infection and Immunity 80 5 1681 1689 |
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Open Polar |
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ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Infectious Diseases Immunology Microbiology Parasitology |
spellingShingle |
Infectious Diseases Immunology Microbiology Parasitology Bjelland, Ane Mohn Sørum, Henning Tegegne, Daget Ayana Winther-Larsen, Hanne C. Willassen, Nils Peder Hansen, Hilde LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence |
topic_facet |
Infectious Diseases Immunology Microbiology Parasitology |
description |
ABSTRACT Vibrio ( Aliivibrio ) salmonicida is the causal agent of cold-water vibriosis, a fatal bacterial septicemia primarily of farmed salmonid fish. The molecular mechanisms of invasion, colonization, and growth of V. salmonicida in the host are still largely unknown, and few virulence factors have been identified. Quorum sensing (QS) is a cell-to-cell communication system known to regulate virulence and other activities in several bacterial species. The genome of V. salmonicida LFI1238 encodes products presumably involved in several QS systems. In this study, the gene encoding LitR, a homolog of the master regulator of QS in V. fischeri , was deleted. Compared to the parental strain, the litR mutant showed increased motility, adhesion, cell-to-cell aggregation, and biofilm formation. Furthermore, the litR mutant produced less cryptic bioluminescence, whereas production of acylhomoserine lactones was unaffected. Our results also indicate a salinity-sensitive regulation of LitR. Finally, reduced mortality was observed in Atlantic salmon infected with the litR mutant, implying that the fish were more susceptible to infection with the wild type than with the mutant strain. We hypothesize that LitR inhibits biofilm formation and favors planktonic growth, with the latter being more adapted for pathogenesis in the fish host. |
author2 |
Camilli, A. |
format |
Article in Journal/Newspaper |
author |
Bjelland, Ane Mohn Sørum, Henning Tegegne, Daget Ayana Winther-Larsen, Hanne C. Willassen, Nils Peder Hansen, Hilde |
author_facet |
Bjelland, Ane Mohn Sørum, Henning Tegegne, Daget Ayana Winther-Larsen, Hanne C. Willassen, Nils Peder Hansen, Hilde |
author_sort |
Bjelland, Ane Mohn |
title |
LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence |
title_short |
LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence |
title_full |
LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence |
title_fullStr |
LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence |
title_full_unstemmed |
LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence |
title_sort |
litr of vibrio salmonicida is a salinity-sensitive quorum-sensing regulator of phenotypes involved in host interactions and virulence |
publisher |
American Society for Microbiology |
publishDate |
2012 |
url |
http://dx.doi.org/10.1128/iai.06038-11 https://journals.asm.org/doi/pdf/10.1128/IAI.06038-11 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Infection and Immunity volume 80, issue 5, page 1681-1689 ISSN 0019-9567 1098-5522 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/iai.06038-11 |
container_title |
Infection and Immunity |
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80 |
container_issue |
5 |
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1681 |
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1689 |
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1781696697675546624 |