Contribution of Type IV Pili to the Virulence of Aeromonas salmonicida subsp. salmonicida in Atlantic Salmon ( Salmo salar L.)
ABSTRACT Aeromonas salmonicida subsp. salmonicida , a bacterial pathogen of Atlantic salmon, has no visible pili, yet its genome contains genes for three type IV pilus systems. One system, Tap, is similar to the Pseudomonas aeruginosa Pil system, and a second, Flp, resembles the Actinobacillus actin...
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crasmicro:10.1128/iai.01019-07 2024-04-07T07:51:01+00:00 Contribution of Type IV Pili to the Virulence of Aeromonas salmonicida subsp. salmonicida in Atlantic Salmon ( Salmo salar L.) Boyd, Jessica M. Dacanay, Andrew Knickle, Leah C. Touhami, Ahmed Brown, Laura L. Jericho, Manfred H. Johnson, Stewart C. Reith, Michael 2008 http://dx.doi.org/10.1128/iai.01019-07 https://journals.asm.org/doi/pdf/10.1128/IAI.01019-07 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Infection and Immunity volume 76, issue 4, page 1445-1455 ISSN 0019-9567 1098-5522 Infectious Diseases Immunology Microbiology Parasitology journal-article 2008 crasmicro https://doi.org/10.1128/iai.01019-07 2024-03-08T00:23:16Z ABSTRACT Aeromonas salmonicida subsp. salmonicida , a bacterial pathogen of Atlantic salmon, has no visible pili, yet its genome contains genes for three type IV pilus systems. One system, Tap, is similar to the Pseudomonas aeruginosa Pil system, and a second, Flp, resembles the Actinobacillus actinomycetemcomitans Flp pilus, while the third has homology to the mannose-sensitive hemagglutinin pilus of Vibrio cholerae . The latter system is likely nonfunctional since eight genes, including the gene encoding the main pilin subunit, are deleted compared with the orthologous V. cholerae locus. The first two systems were characterized to investigate their expression and role in pathogenesis. The pili of A. salmonicida subsp. salmonicida were imaged using atomic force microscopy and Tap- and Flp-overexpressing strains. The Tap pili appeared to be polar, while the Flp pili appeared to be peritrichous. Strains deficient in tap and/or flp were used in live bacterial challenges of Atlantic salmon, which showed that the Tap pilus made a moderate contribution to virulence, while the Flp pilus made little or no contribution. Delivery of the tap mutant by immersion resulted in reduced cumulative morbidity compared with the cumulative morbidity observed with the wild-type strain; however, delivery by intraperitoneal injection resulted in cumulative morbidity similar to that of the wild type. Unlike the pili of other piliated bacterial pathogens, A. salmonicida subsp. salmonicida type IV pili are not absolutely required for virulence in Atlantic salmon. Significant differences in the behavior of the two mutant strains indicated that the two pilus systems are not redundant. Article in Journal/Newspaper Atlantic salmon Salmo salar ASM Journals (American Society for Microbiology) Infection and Immunity 76 4 1445 1455 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
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English |
topic |
Infectious Diseases Immunology Microbiology Parasitology |
spellingShingle |
Infectious Diseases Immunology Microbiology Parasitology Boyd, Jessica M. Dacanay, Andrew Knickle, Leah C. Touhami, Ahmed Brown, Laura L. Jericho, Manfred H. Johnson, Stewart C. Reith, Michael Contribution of Type IV Pili to the Virulence of Aeromonas salmonicida subsp. salmonicida in Atlantic Salmon ( Salmo salar L.) |
topic_facet |
Infectious Diseases Immunology Microbiology Parasitology |
description |
ABSTRACT Aeromonas salmonicida subsp. salmonicida , a bacterial pathogen of Atlantic salmon, has no visible pili, yet its genome contains genes for three type IV pilus systems. One system, Tap, is similar to the Pseudomonas aeruginosa Pil system, and a second, Flp, resembles the Actinobacillus actinomycetemcomitans Flp pilus, while the third has homology to the mannose-sensitive hemagglutinin pilus of Vibrio cholerae . The latter system is likely nonfunctional since eight genes, including the gene encoding the main pilin subunit, are deleted compared with the orthologous V. cholerae locus. The first two systems were characterized to investigate their expression and role in pathogenesis. The pili of A. salmonicida subsp. salmonicida were imaged using atomic force microscopy and Tap- and Flp-overexpressing strains. The Tap pili appeared to be polar, while the Flp pili appeared to be peritrichous. Strains deficient in tap and/or flp were used in live bacterial challenges of Atlantic salmon, which showed that the Tap pilus made a moderate contribution to virulence, while the Flp pilus made little or no contribution. Delivery of the tap mutant by immersion resulted in reduced cumulative morbidity compared with the cumulative morbidity observed with the wild-type strain; however, delivery by intraperitoneal injection resulted in cumulative morbidity similar to that of the wild type. Unlike the pili of other piliated bacterial pathogens, A. salmonicida subsp. salmonicida type IV pili are not absolutely required for virulence in Atlantic salmon. Significant differences in the behavior of the two mutant strains indicated that the two pilus systems are not redundant. |
format |
Article in Journal/Newspaper |
author |
Boyd, Jessica M. Dacanay, Andrew Knickle, Leah C. Touhami, Ahmed Brown, Laura L. Jericho, Manfred H. Johnson, Stewart C. Reith, Michael |
author_facet |
Boyd, Jessica M. Dacanay, Andrew Knickle, Leah C. Touhami, Ahmed Brown, Laura L. Jericho, Manfred H. Johnson, Stewart C. Reith, Michael |
author_sort |
Boyd, Jessica M. |
title |
Contribution of Type IV Pili to the Virulence of Aeromonas salmonicida subsp. salmonicida in Atlantic Salmon ( Salmo salar L.) |
title_short |
Contribution of Type IV Pili to the Virulence of Aeromonas salmonicida subsp. salmonicida in Atlantic Salmon ( Salmo salar L.) |
title_full |
Contribution of Type IV Pili to the Virulence of Aeromonas salmonicida subsp. salmonicida in Atlantic Salmon ( Salmo salar L.) |
title_fullStr |
Contribution of Type IV Pili to the Virulence of Aeromonas salmonicida subsp. salmonicida in Atlantic Salmon ( Salmo salar L.) |
title_full_unstemmed |
Contribution of Type IV Pili to the Virulence of Aeromonas salmonicida subsp. salmonicida in Atlantic Salmon ( Salmo salar L.) |
title_sort |
contribution of type iv pili to the virulence of aeromonas salmonicida subsp. salmonicida in atlantic salmon ( salmo salar l.) |
publisher |
American Society for Microbiology |
publishDate |
2008 |
url |
http://dx.doi.org/10.1128/iai.01019-07 https://journals.asm.org/doi/pdf/10.1128/IAI.01019-07 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Infection and Immunity volume 76, issue 4, page 1445-1455 ISSN 0019-9567 1098-5522 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/iai.01019-07 |
container_title |
Infection and Immunity |
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76 |
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4 |
container_start_page |
1445 |
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1455 |
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1795665856595755008 |