Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ + CD83 + Antigen-Presenting Cells
ABSTRACT The histozoic myxozoan parasite Kudoa thyrsites causes postmortem myoliquefaction and is responsible for economic losses to salmon aquaculture in the Pacific Northwest. Despite its importance, little is known about the host-parasite relationship, including the host response to infection. Th...
Published in: | Infection and Immunity |
---|---|
Main Authors: | , , , , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
American Society for Microbiology
2018
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1128/iai.00556-17 https://journals.asm.org/doi/pdf/10.1128/IAI.00556-17 |
id |
crasmicro:10.1128/iai.00556-17 |
---|---|
record_format |
openpolar |
spelling |
crasmicro:10.1128/iai.00556-17 2024-04-28T08:13:24+00:00 Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ + CD83 + Antigen-Presenting Cells Braden, Laura M. Rasmussen, Karina J. Purcell, Sara L. Ellis, Lauren Mahony, Amelia Cho, Steven Whyte, Shona K. Jones, Simon R. M. Fast, Mark D. Appleton, Judith A. 2018 http://dx.doi.org/10.1128/iai.00556-17 https://journals.asm.org/doi/pdf/10.1128/IAI.00556-17 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Infection and Immunity volume 86, issue 1 ISSN 0019-9567 1098-5522 Infectious Diseases Immunology Microbiology Parasitology journal-article 2018 crasmicro https://doi.org/10.1128/iai.00556-17 2024-04-09T06:52:51Z ABSTRACT The histozoic myxozoan parasite Kudoa thyrsites causes postmortem myoliquefaction and is responsible for economic losses to salmon aquaculture in the Pacific Northwest. Despite its importance, little is known about the host-parasite relationship, including the host response to infection. The present work sought to characterize the immune response in Atlantic salmon during infection, recovery, and reexposure to K. thyrsites . After exposure to infective seawater, infected and uninfected smolts were sampled three times over 4,275 degree-days. Histological analysis revealed infection severity decreased over time in exposed fish, while in controls there was no evidence of infection. Following a secondary exposure of all fish, severity of infection in the controls was similar to that measured in exposed fish at the first sampling time but was significantly reduced in reexposed fish, suggesting the acquisition of protective immunity. Using immunohistochemistry, we detected a population of MHIIβ + cells in infected muscle that followed a pattern of abundance concordant with parasite prevalence. Infiltration of these cells into infected myocytes preceded destruction of the plasmodium and dissemination of myxospores. Dual labeling indicated a majority of these cells were CD83 + /MHIIβ + . Using reverse transcription-quantitative PCR, we detected significant induction of cellular effectors, including macrophage/dendritic cells ( mhii / cd83 / mcsf ), B cells ( igm / igt ), and cytotoxic T cells ( cd8 / nkl ), in the musculature of infected fish. These data support a role for cellular effectors such as antigen-presenting cells (monocyte/macrophage and dendritic cells) along with B and T cells in the acquired protective immune response of Atlantic salmon against K. thyrsites . Article in Journal/Newspaper Atlantic salmon Salmo salar ASM Journals (American Society for Microbiology) Infection and Immunity 86 1 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
topic |
Infectious Diseases Immunology Microbiology Parasitology |
spellingShingle |
Infectious Diseases Immunology Microbiology Parasitology Braden, Laura M. Rasmussen, Karina J. Purcell, Sara L. Ellis, Lauren Mahony, Amelia Cho, Steven Whyte, Shona K. Jones, Simon R. M. Fast, Mark D. Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ + CD83 + Antigen-Presenting Cells |
topic_facet |
Infectious Diseases Immunology Microbiology Parasitology |
description |
ABSTRACT The histozoic myxozoan parasite Kudoa thyrsites causes postmortem myoliquefaction and is responsible for economic losses to salmon aquaculture in the Pacific Northwest. Despite its importance, little is known about the host-parasite relationship, including the host response to infection. The present work sought to characterize the immune response in Atlantic salmon during infection, recovery, and reexposure to K. thyrsites . After exposure to infective seawater, infected and uninfected smolts were sampled three times over 4,275 degree-days. Histological analysis revealed infection severity decreased over time in exposed fish, while in controls there was no evidence of infection. Following a secondary exposure of all fish, severity of infection in the controls was similar to that measured in exposed fish at the first sampling time but was significantly reduced in reexposed fish, suggesting the acquisition of protective immunity. Using immunohistochemistry, we detected a population of MHIIβ + cells in infected muscle that followed a pattern of abundance concordant with parasite prevalence. Infiltration of these cells into infected myocytes preceded destruction of the plasmodium and dissemination of myxospores. Dual labeling indicated a majority of these cells were CD83 + /MHIIβ + . Using reverse transcription-quantitative PCR, we detected significant induction of cellular effectors, including macrophage/dendritic cells ( mhii / cd83 / mcsf ), B cells ( igm / igt ), and cytotoxic T cells ( cd8 / nkl ), in the musculature of infected fish. These data support a role for cellular effectors such as antigen-presenting cells (monocyte/macrophage and dendritic cells) along with B and T cells in the acquired protective immune response of Atlantic salmon against K. thyrsites . |
author2 |
Appleton, Judith A. |
format |
Article in Journal/Newspaper |
author |
Braden, Laura M. Rasmussen, Karina J. Purcell, Sara L. Ellis, Lauren Mahony, Amelia Cho, Steven Whyte, Shona K. Jones, Simon R. M. Fast, Mark D. |
author_facet |
Braden, Laura M. Rasmussen, Karina J. Purcell, Sara L. Ellis, Lauren Mahony, Amelia Cho, Steven Whyte, Shona K. Jones, Simon R. M. Fast, Mark D. |
author_sort |
Braden, Laura M. |
title |
Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ + CD83 + Antigen-Presenting Cells |
title_short |
Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ + CD83 + Antigen-Presenting Cells |
title_full |
Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ + CD83 + Antigen-Presenting Cells |
title_fullStr |
Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ + CD83 + Antigen-Presenting Cells |
title_full_unstemmed |
Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ + CD83 + Antigen-Presenting Cells |
title_sort |
acquired protective immunity in atlantic salmon salmo salar against the myxozoan kudoa thyrsites involves induction of mhiiβ + cd83 + antigen-presenting cells |
publisher |
American Society for Microbiology |
publishDate |
2018 |
url |
http://dx.doi.org/10.1128/iai.00556-17 https://journals.asm.org/doi/pdf/10.1128/IAI.00556-17 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Infection and Immunity volume 86, issue 1 ISSN 0019-9567 1098-5522 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/iai.00556-17 |
container_title |
Infection and Immunity |
container_volume |
86 |
container_issue |
1 |
_version_ |
1797579913248636928 |