Experimental Infection of Reindeer with Cervid Herpesvirus 2
ABSTRACT Cervid herpesvirus 2 (CvHV2) has been isolated from reindeer ( Rangifer tarandus tarandus ), and serological data indicate that in reindeer this virus is endemic in Fennoscandia, Alaska, Canada, and Greenland. CvHV2 has been described as a cause of subclinical genital infections in reindeer...
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crasmicro:10.1128/cvi.00218-09 2023-10-09T21:51:26+02:00 Experimental Infection of Reindeer with Cervid Herpesvirus 2 das Neves, Carlos G. Mørk, Torill Godfroid, Jacques Sørensen, Karen K. Breines, Eva Hareide, Ellinor Thiry, Julien Rimstad, Espen Thiry, Etienne Tryland, Morten 2009 http://dx.doi.org/10.1128/cvi.00218-09 https://journals.asm.org/doi/pdf/10.1128/CVI.00218-09 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Clinical and Vaccine Immunology volume 16, issue 12, page 1758-1765 ISSN 1556-6811 1556-679X Microbiology (medical) Clinical Biochemistry Immunology Immunology and Allergy journal-article 2009 crasmicro https://doi.org/10.1128/cvi.00218-09 2023-09-15T05:46:11Z ABSTRACT Cervid herpesvirus 2 (CvHV2) has been isolated from reindeer ( Rangifer tarandus tarandus ), and serological data indicate that in reindeer this virus is endemic in Fennoscandia, Alaska, Canada, and Greenland. CvHV2 has been described as a cause of subclinical genital infections in reindeer, but little information on primary infections exists. In this study, six seronegative and presumably pregnant reindeer were allocated to one of two groups. Two animals were inoculated with CvHV2 intratracheally, and two animals intravaginally, with one control animal in each group receiving sterile water. Mild hyperthermia and serous discharges from the vagina and nose were observed. No abortions were recorded, but one calf died shortly after birth. Inoculated animals seroconverted and had neutralizing antibodies after days 7 to 10 postinfection. CvHV2 was detected by PCR in nasal and vaginal swabs from animals in both groups but could be isolated only from nasal swabs in the respiratory group and from vaginal swabs in the genital group. CvHV2 was detected by PCR in various organs and tissues postmortem. In control animals, the virus could not be isolated in spite of PCR-positive nasal and vaginal swab samples and some degree of positive immunostaining. One of the animals that were inoculated intratracheally developed a hemorrhagic, necrotizing bronchopneumonia, which was CvHV2 positive by PCR and immunohistochemistry. We conclude that CvHV2 can cause systemic infection, that both genital and respiratory inoculations can lead to virus shedding, and that the virus can infect the fetus in utero. Article in Journal/Newspaper Fennoscandia Greenland Rangifer tarandus Alaska ASM Journals (American Society for Microbiology - via Crossref) Canada Greenland Clinical and Vaccine Immunology 16 12 1758 1765 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Microbiology (medical) Clinical Biochemistry Immunology Immunology and Allergy |
spellingShingle |
Microbiology (medical) Clinical Biochemistry Immunology Immunology and Allergy das Neves, Carlos G. Mørk, Torill Godfroid, Jacques Sørensen, Karen K. Breines, Eva Hareide, Ellinor Thiry, Julien Rimstad, Espen Thiry, Etienne Tryland, Morten Experimental Infection of Reindeer with Cervid Herpesvirus 2 |
topic_facet |
Microbiology (medical) Clinical Biochemistry Immunology Immunology and Allergy |
description |
ABSTRACT Cervid herpesvirus 2 (CvHV2) has been isolated from reindeer ( Rangifer tarandus tarandus ), and serological data indicate that in reindeer this virus is endemic in Fennoscandia, Alaska, Canada, and Greenland. CvHV2 has been described as a cause of subclinical genital infections in reindeer, but little information on primary infections exists. In this study, six seronegative and presumably pregnant reindeer were allocated to one of two groups. Two animals were inoculated with CvHV2 intratracheally, and two animals intravaginally, with one control animal in each group receiving sterile water. Mild hyperthermia and serous discharges from the vagina and nose were observed. No abortions were recorded, but one calf died shortly after birth. Inoculated animals seroconverted and had neutralizing antibodies after days 7 to 10 postinfection. CvHV2 was detected by PCR in nasal and vaginal swabs from animals in both groups but could be isolated only from nasal swabs in the respiratory group and from vaginal swabs in the genital group. CvHV2 was detected by PCR in various organs and tissues postmortem. In control animals, the virus could not be isolated in spite of PCR-positive nasal and vaginal swab samples and some degree of positive immunostaining. One of the animals that were inoculated intratracheally developed a hemorrhagic, necrotizing bronchopneumonia, which was CvHV2 positive by PCR and immunohistochemistry. We conclude that CvHV2 can cause systemic infection, that both genital and respiratory inoculations can lead to virus shedding, and that the virus can infect the fetus in utero. |
format |
Article in Journal/Newspaper |
author |
das Neves, Carlos G. Mørk, Torill Godfroid, Jacques Sørensen, Karen K. Breines, Eva Hareide, Ellinor Thiry, Julien Rimstad, Espen Thiry, Etienne Tryland, Morten |
author_facet |
das Neves, Carlos G. Mørk, Torill Godfroid, Jacques Sørensen, Karen K. Breines, Eva Hareide, Ellinor Thiry, Julien Rimstad, Espen Thiry, Etienne Tryland, Morten |
author_sort |
das Neves, Carlos G. |
title |
Experimental Infection of Reindeer with Cervid Herpesvirus 2 |
title_short |
Experimental Infection of Reindeer with Cervid Herpesvirus 2 |
title_full |
Experimental Infection of Reindeer with Cervid Herpesvirus 2 |
title_fullStr |
Experimental Infection of Reindeer with Cervid Herpesvirus 2 |
title_full_unstemmed |
Experimental Infection of Reindeer with Cervid Herpesvirus 2 |
title_sort |
experimental infection of reindeer with cervid herpesvirus 2 |
publisher |
American Society for Microbiology |
publishDate |
2009 |
url |
http://dx.doi.org/10.1128/cvi.00218-09 https://journals.asm.org/doi/pdf/10.1128/CVI.00218-09 |
geographic |
Canada Greenland |
geographic_facet |
Canada Greenland |
genre |
Fennoscandia Greenland Rangifer tarandus Alaska |
genre_facet |
Fennoscandia Greenland Rangifer tarandus Alaska |
op_source |
Clinical and Vaccine Immunology volume 16, issue 12, page 1758-1765 ISSN 1556-6811 1556-679X |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/cvi.00218-09 |
container_title |
Clinical and Vaccine Immunology |
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16 |
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12 |
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1758 |
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1765 |
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1779314540370984960 |