16S rRNA Sequences and Differences in Bacteria Isolated from the Muztag Ata Glacier at Increasing Depths
ABSTRACT Small subunit 16S rRNA sequences, growth temperatures, and phylogenetic relationships have been established for 129 bacterial isolates recovered under aerobic growth conditions from different regions of a 22-m ice core from the Muztag Ata Mountain glacier on the Pamirs Plateau (China). Only...
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Online Access: | http://dx.doi.org/10.1128/aem.71.8.4619-4627.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.8.4619-4627.2005 |
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crasmicro:10.1128/aem.71.8.4619-4627.2005 2024-09-15T18:12:02+00:00 16S rRNA Sequences and Differences in Bacteria Isolated from the Muztag Ata Glacier at Increasing Depths Xiang, Shurong Yao, Tandong An, Lizhe Xu, Bingliang Wang, Junxia 2005 http://dx.doi.org/10.1128/aem.71.8.4619-4627.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.8.4619-4627.2005 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 71, issue 8, page 4619-4627 ISSN 0099-2240 1098-5336 journal-article 2005 crasmicro https://doi.org/10.1128/aem.71.8.4619-4627.2005 2024-08-26T04:06:40Z ABSTRACT Small subunit 16S rRNA sequences, growth temperatures, and phylogenetic relationships have been established for 129 bacterial isolates recovered under aerobic growth conditions from different regions of a 22-m ice core from the Muztag Ata Mountain glacier on the Pamirs Plateau (China). Only 11% were psychrophiles (grew at 2°C or −2°C up to ∼20°C), although the majority (82%) were psychrotolerant (grew at 2°C or −2°C up to 37°C). The majority of the isolates had 16S rRNA sequences similar to previously determined sequences, ranging from 85% to 100% identical to database sequences. Based on their 16S rRNA sequences, 42.6% of the isolates were high-G+C (HGC) gram-positive bacteria, 23.3% wereγ - Proteobacteria , 14.7% were α- Proteobacteria , 14.7% were Flavobacteria , and 4.7% were low-G+C (LGC) gram-positive bacteria. There were clear differences in the depth distribution, with Proteobacteria , HGC/ Cytophaga - Flavobacterium - Bacteroides (CFB), Proteobacteria , LGC/CFB/HGC, Cryobacterium psychrophilum , HGC/CFB, Proteobacteria /HGC/CFB, and HGC/CFB being the predominant isolates from ice that originated from 2.7 to 3.8, 6.2, 7.5, 8.3, 9.0, 9.7, 12.5, and 15.3 m below the surface, respectively. This layered distribution of bacterial isolates presumably reflects both differences in bacteria inhabiting the glacier's surface, differences in bacteria deposited serendipitously on the glacier's surface by wind and snowfall, and nutrient availability within the ice. Article in Journal/Newspaper ice core ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 71 8 4619 4627 |
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ASM Journals (American Society for Microbiology) |
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English |
description |
ABSTRACT Small subunit 16S rRNA sequences, growth temperatures, and phylogenetic relationships have been established for 129 bacterial isolates recovered under aerobic growth conditions from different regions of a 22-m ice core from the Muztag Ata Mountain glacier on the Pamirs Plateau (China). Only 11% were psychrophiles (grew at 2°C or −2°C up to ∼20°C), although the majority (82%) were psychrotolerant (grew at 2°C or −2°C up to 37°C). The majority of the isolates had 16S rRNA sequences similar to previously determined sequences, ranging from 85% to 100% identical to database sequences. Based on their 16S rRNA sequences, 42.6% of the isolates were high-G+C (HGC) gram-positive bacteria, 23.3% wereγ - Proteobacteria , 14.7% were α- Proteobacteria , 14.7% were Flavobacteria , and 4.7% were low-G+C (LGC) gram-positive bacteria. There were clear differences in the depth distribution, with Proteobacteria , HGC/ Cytophaga - Flavobacterium - Bacteroides (CFB), Proteobacteria , LGC/CFB/HGC, Cryobacterium psychrophilum , HGC/CFB, Proteobacteria /HGC/CFB, and HGC/CFB being the predominant isolates from ice that originated from 2.7 to 3.8, 6.2, 7.5, 8.3, 9.0, 9.7, 12.5, and 15.3 m below the surface, respectively. This layered distribution of bacterial isolates presumably reflects both differences in bacteria inhabiting the glacier's surface, differences in bacteria deposited serendipitously on the glacier's surface by wind and snowfall, and nutrient availability within the ice. |
format |
Article in Journal/Newspaper |
author |
Xiang, Shurong Yao, Tandong An, Lizhe Xu, Bingliang Wang, Junxia |
spellingShingle |
Xiang, Shurong Yao, Tandong An, Lizhe Xu, Bingliang Wang, Junxia 16S rRNA Sequences and Differences in Bacteria Isolated from the Muztag Ata Glacier at Increasing Depths |
author_facet |
Xiang, Shurong Yao, Tandong An, Lizhe Xu, Bingliang Wang, Junxia |
author_sort |
Xiang, Shurong |
title |
16S rRNA Sequences and Differences in Bacteria Isolated from the Muztag Ata Glacier at Increasing Depths |
title_short |
16S rRNA Sequences and Differences in Bacteria Isolated from the Muztag Ata Glacier at Increasing Depths |
title_full |
16S rRNA Sequences and Differences in Bacteria Isolated from the Muztag Ata Glacier at Increasing Depths |
title_fullStr |
16S rRNA Sequences and Differences in Bacteria Isolated from the Muztag Ata Glacier at Increasing Depths |
title_full_unstemmed |
16S rRNA Sequences and Differences in Bacteria Isolated from the Muztag Ata Glacier at Increasing Depths |
title_sort |
16s rrna sequences and differences in bacteria isolated from the muztag ata glacier at increasing depths |
publisher |
American Society for Microbiology |
publishDate |
2005 |
url |
http://dx.doi.org/10.1128/aem.71.8.4619-4627.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.8.4619-4627.2005 |
genre |
ice core |
genre_facet |
ice core |
op_source |
Applied and Environmental Microbiology volume 71, issue 8, page 4619-4627 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.71.8.4619-4627.2005 |
container_title |
Applied and Environmental Microbiology |
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71 |
container_issue |
8 |
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4619 |
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4627 |
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1810449617668538368 |