Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture
ABSTRACT The sea ice microbial community plays a key role in the productivity of the Southern Ocean. Exopolysaccharide (EPS) is a major component of the exopolymer secreted by many marine bacteria to enhance survival and is abundant in sea ice brine channels, but little is known about its function t...
Published in: | Applied and Environmental Microbiology |
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Language: | English |
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American Society for Microbiology
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Online Access: | http://dx.doi.org/10.1128/aem.71.7.3519-3523.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.7.3519-3523.2005 |
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crasmicro:10.1128/aem.71.7.3519-3523.2005 2024-10-13T14:03:03+00:00 Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture Nichols, Carol Mancuso Bowman, John P. Guezennec, Jean 2005 http://dx.doi.org/10.1128/aem.71.7.3519-3523.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.7.3519-3523.2005 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 71, issue 7, page 3519-3523 ISSN 0099-2240 1098-5336 journal-article 2005 crasmicro https://doi.org/10.1128/aem.71.7.3519-3523.2005 2024-09-17T04:07:50Z ABSTRACT The sea ice microbial community plays a key role in the productivity of the Southern Ocean. Exopolysaccharide (EPS) is a major component of the exopolymer secreted by many marine bacteria to enhance survival and is abundant in sea ice brine channels, but little is known about its function there. This study investigated the effects of temperature on EPS production in batch culture by CAM025, a marine bacterium isolated from sea ice sampled from the Southern Ocean. Previous studies have shown that CAM025 is a member of the genus Pseudoalteromonas and therefore belongs to a group found to be abundant in sea ice by culture-dependent and -independent techniques. Batch cultures were grown at −2°C, 10°C, and 20°C, and cell number, optical density, pH, glucose concentration, and viscosity were monitored. The yield of EPS at −2°C and 10°C was 30 times higher than at 20°C, which is the optimum growth temperature for many psychrotolerant strains. EPS may have a cryoprotective role in brine channels of sea ice, where extremes of high salinity and low temperature impose pressures on microbial growth and survival. The EPS produced at −2°C and 10°C had a higher uronic acid content than that produced at 20°C. The availability of iron as a trace metal is of critical importance in the Southern Ocean, where it is known to limit primary production. EPS from strain CAM025 is polyanionic and may bind dissolved cations such at trace metals, and therefore the presence of bacterial EPS in the Antarctic marine environment may have important ecological implications. Article in Journal/Newspaper Antarc* Antarctic Sea ice Southern Ocean ASM Journals (American Society for Microbiology) Antarctic Southern Ocean The Antarctic Applied and Environmental Microbiology 71 7 3519 3523 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT The sea ice microbial community plays a key role in the productivity of the Southern Ocean. Exopolysaccharide (EPS) is a major component of the exopolymer secreted by many marine bacteria to enhance survival and is abundant in sea ice brine channels, but little is known about its function there. This study investigated the effects of temperature on EPS production in batch culture by CAM025, a marine bacterium isolated from sea ice sampled from the Southern Ocean. Previous studies have shown that CAM025 is a member of the genus Pseudoalteromonas and therefore belongs to a group found to be abundant in sea ice by culture-dependent and -independent techniques. Batch cultures were grown at −2°C, 10°C, and 20°C, and cell number, optical density, pH, glucose concentration, and viscosity were monitored. The yield of EPS at −2°C and 10°C was 30 times higher than at 20°C, which is the optimum growth temperature for many psychrotolerant strains. EPS may have a cryoprotective role in brine channels of sea ice, where extremes of high salinity and low temperature impose pressures on microbial growth and survival. The EPS produced at −2°C and 10°C had a higher uronic acid content than that produced at 20°C. The availability of iron as a trace metal is of critical importance in the Southern Ocean, where it is known to limit primary production. EPS from strain CAM025 is polyanionic and may bind dissolved cations such at trace metals, and therefore the presence of bacterial EPS in the Antarctic marine environment may have important ecological implications. |
format |
Article in Journal/Newspaper |
author |
Nichols, Carol Mancuso Bowman, John P. Guezennec, Jean |
spellingShingle |
Nichols, Carol Mancuso Bowman, John P. Guezennec, Jean Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture |
author_facet |
Nichols, Carol Mancuso Bowman, John P. Guezennec, Jean |
author_sort |
Nichols, Carol Mancuso |
title |
Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture |
title_short |
Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture |
title_full |
Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture |
title_fullStr |
Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture |
title_full_unstemmed |
Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture |
title_sort |
effects of incubation temperature on growth and production of exopolysaccharides by an antarctic sea ice bacterium grown in batch culture |
publisher |
American Society for Microbiology |
publishDate |
2005 |
url |
http://dx.doi.org/10.1128/aem.71.7.3519-3523.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.7.3519-3523.2005 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Sea ice Southern Ocean |
op_source |
Applied and Environmental Microbiology volume 71, issue 7, page 3519-3523 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.71.7.3519-3523.2005 |
container_title |
Applied and Environmental Microbiology |
container_volume |
71 |
container_issue |
7 |
container_start_page |
3519 |
op_container_end_page |
3523 |
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1812819478562471936 |