Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes
ABSTRACT The microbial diversity of intertidal hot springs on the seashore of northwest Iceland was examined by combining directed in situ enrichments, artificial support colonization, and mat sampling. Analysis of 16S rRNA genes revealed the presence of clones related to both marine and terrestrial...
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American Society for Microbiology
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Online Access: | http://dx.doi.org/10.1128/aem.71.5.2771-2776.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.5.2771-2776.2005 |
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crasmicro:10.1128/aem.71.5.2771-2776.2005 2024-06-23T07:54:01+00:00 Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes Hobel, Cédric F. V. Marteinsson, Viggó T. Hreggvidsson, Gudmundur Ó. Kristjánsson, Jakob K. 2005 http://dx.doi.org/10.1128/aem.71.5.2771-2776.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.5.2771-2776.2005 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 71, issue 5, page 2771-2776 ISSN 0099-2240 1098-5336 journal-article 2005 crasmicro https://doi.org/10.1128/aem.71.5.2771-2776.2005 2024-06-10T04:07:31Z ABSTRACT The microbial diversity of intertidal hot springs on the seashore of northwest Iceland was examined by combining directed in situ enrichments, artificial support colonization, and mat sampling. Analysis of 16S rRNA genes revealed the presence of clones related to both marine and terrestrial, thermophilic, mesophilic, and psychrophilic microorganisms scattered among 11 bacterial divisions. No archaea were found. The species composition of the enrichments was affected by the length of the hot periods experienced at low tide and was very different from those found in the biomass. A total of 36 chitinase genes were detected by molecular screening of the samples with degenerate primers for glycoside hydrolase family 18. The chitinase gene diversity was at least twofold higher in the enrichment samples than in the controls, indicating that a much higher diversity of hydrolytic genes can be accessed with this approach. Article in Journal/Newspaper Iceland ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 71 5 2771 2776 |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
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English |
description |
ABSTRACT The microbial diversity of intertidal hot springs on the seashore of northwest Iceland was examined by combining directed in situ enrichments, artificial support colonization, and mat sampling. Analysis of 16S rRNA genes revealed the presence of clones related to both marine and terrestrial, thermophilic, mesophilic, and psychrophilic microorganisms scattered among 11 bacterial divisions. No archaea were found. The species composition of the enrichments was affected by the length of the hot periods experienced at low tide and was very different from those found in the biomass. A total of 36 chitinase genes were detected by molecular screening of the samples with degenerate primers for glycoside hydrolase family 18. The chitinase gene diversity was at least twofold higher in the enrichment samples than in the controls, indicating that a much higher diversity of hydrolytic genes can be accessed with this approach. |
format |
Article in Journal/Newspaper |
author |
Hobel, Cédric F. V. Marteinsson, Viggó T. Hreggvidsson, Gudmundur Ó. Kristjánsson, Jakob K. |
spellingShingle |
Hobel, Cédric F. V. Marteinsson, Viggó T. Hreggvidsson, Gudmundur Ó. Kristjánsson, Jakob K. Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes |
author_facet |
Hobel, Cédric F. V. Marteinsson, Viggó T. Hreggvidsson, Gudmundur Ó. Kristjánsson, Jakob K. |
author_sort |
Hobel, Cédric F. V. |
title |
Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes |
title_short |
Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes |
title_full |
Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes |
title_fullStr |
Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes |
title_full_unstemmed |
Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes |
title_sort |
investigation of the microbial ecology of intertidal hot springs by using diversity analysis of 16s rrna and chitinase genes |
publisher |
American Society for Microbiology |
publishDate |
2005 |
url |
http://dx.doi.org/10.1128/aem.71.5.2771-2776.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.5.2771-2776.2005 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Applied and Environmental Microbiology volume 71, issue 5, page 2771-2776 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.71.5.2771-2776.2005 |
container_title |
Applied and Environmental Microbiology |
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71 |
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5 |
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2771 |
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2776 |
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1802645941829763072 |