Chitinase Genes in Lake Sediments of Ardley Island, Antarctica
ABSTRACT A sediment core spanning approximately 1,600 years was collected from a lake on Ardley Island, Antarctica. The sediment core had been greatly influenced by penguin guano. Using molecular methods, the chitinolytic bacterial community along the sediment core was studied over its entire length...
Published in: | Applied and Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1128/aem.71.12.7904-7909.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.12.7904-7909.2005 |
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crasmicro:10.1128/aem.71.12.7904-7909.2005 2024-09-15T17:43:32+00:00 Chitinase Genes in Lake Sediments of Ardley Island, Antarctica Xiao, Xiang Yin, Xuebin Lin, Jian Sun, Liguang You, Ziyong Wang, Peng Wang, Fengping 2005 http://dx.doi.org/10.1128/aem.71.12.7904-7909.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.12.7904-7909.2005 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 71, issue 12, page 7904-7909 ISSN 0099-2240 1098-5336 journal-article 2005 crasmicro https://doi.org/10.1128/aem.71.12.7904-7909.2005 2024-08-19T04:06:21Z ABSTRACT A sediment core spanning approximately 1,600 years was collected from a lake on Ardley Island, Antarctica. The sediment core had been greatly influenced by penguin guano. Using molecular methods, the chitinolytic bacterial community along the sediment core was studied over its entire length. Primers targeting conserved sequences of the catalytic domains of family 18 subgroup A chitinases detected group A chitinases from a wide taxonomic range of bacteria. Using quantitative competitive PCR (QC-PCR), chitinase gene copies in each 1-cm section of the whole sediment column were quantified. QC-PCR determination of the chitinase gene copies indicated significant correlation with phosphorus and total organic carbon concentration, suggesting a historical connection between chitinase gene copies and the amount of penguin guano input into the lake sediment. Most of the chitinase genes cloned from the historic sediment core were novel. Analysis of the chitinase gene diversity in selected sediment layers and in the fresh penguin deposits indicated frequent shifts in the chitinolytic bacterial community over time. Sequence analysis of the 16S rRNA genes of chitinolytic bacteria isolated from the lake sediment revealed that the isolates belonged to Janthinobacterium species, Stenotrophomonas species of γ- Proteobacteria , Cytophaga species of the Cytophaga - Flexibacter - Bacteroides group, and Streptomyces and Norcardiopsis species of Actinobacteria . Chitinase gene fragments were cloned and sequenced from these cultivated chitinolytic bacteria. The phylogeny of the chitinase genes obtained from the isolates did not correspond well to that of the isolates, suggesting acquisition via horizontal gene transfer. Article in Journal/Newspaper Antarc* Antarctica Ardley Island ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 71 12 7904 7909 |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT A sediment core spanning approximately 1,600 years was collected from a lake on Ardley Island, Antarctica. The sediment core had been greatly influenced by penguin guano. Using molecular methods, the chitinolytic bacterial community along the sediment core was studied over its entire length. Primers targeting conserved sequences of the catalytic domains of family 18 subgroup A chitinases detected group A chitinases from a wide taxonomic range of bacteria. Using quantitative competitive PCR (QC-PCR), chitinase gene copies in each 1-cm section of the whole sediment column were quantified. QC-PCR determination of the chitinase gene copies indicated significant correlation with phosphorus and total organic carbon concentration, suggesting a historical connection between chitinase gene copies and the amount of penguin guano input into the lake sediment. Most of the chitinase genes cloned from the historic sediment core were novel. Analysis of the chitinase gene diversity in selected sediment layers and in the fresh penguin deposits indicated frequent shifts in the chitinolytic bacterial community over time. Sequence analysis of the 16S rRNA genes of chitinolytic bacteria isolated from the lake sediment revealed that the isolates belonged to Janthinobacterium species, Stenotrophomonas species of γ- Proteobacteria , Cytophaga species of the Cytophaga - Flexibacter - Bacteroides group, and Streptomyces and Norcardiopsis species of Actinobacteria . Chitinase gene fragments were cloned and sequenced from these cultivated chitinolytic bacteria. The phylogeny of the chitinase genes obtained from the isolates did not correspond well to that of the isolates, suggesting acquisition via horizontal gene transfer. |
format |
Article in Journal/Newspaper |
author |
Xiao, Xiang Yin, Xuebin Lin, Jian Sun, Liguang You, Ziyong Wang, Peng Wang, Fengping |
spellingShingle |
Xiao, Xiang Yin, Xuebin Lin, Jian Sun, Liguang You, Ziyong Wang, Peng Wang, Fengping Chitinase Genes in Lake Sediments of Ardley Island, Antarctica |
author_facet |
Xiao, Xiang Yin, Xuebin Lin, Jian Sun, Liguang You, Ziyong Wang, Peng Wang, Fengping |
author_sort |
Xiao, Xiang |
title |
Chitinase Genes in Lake Sediments of Ardley Island, Antarctica |
title_short |
Chitinase Genes in Lake Sediments of Ardley Island, Antarctica |
title_full |
Chitinase Genes in Lake Sediments of Ardley Island, Antarctica |
title_fullStr |
Chitinase Genes in Lake Sediments of Ardley Island, Antarctica |
title_full_unstemmed |
Chitinase Genes in Lake Sediments of Ardley Island, Antarctica |
title_sort |
chitinase genes in lake sediments of ardley island, antarctica |
publisher |
American Society for Microbiology |
publishDate |
2005 |
url |
http://dx.doi.org/10.1128/aem.71.12.7904-7909.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.12.7904-7909.2005 |
genre |
Antarc* Antarctica Ardley Island |
genre_facet |
Antarc* Antarctica Ardley Island |
op_source |
Applied and Environmental Microbiology volume 71, issue 12, page 7904-7909 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.71.12.7904-7909.2005 |
container_title |
Applied and Environmental Microbiology |
container_volume |
71 |
container_issue |
12 |
container_start_page |
7904 |
op_container_end_page |
7909 |
_version_ |
1810490540197675008 |