Ecology, Inhibitory Activity, and Morphogenesis of a Marine Antagonistic Bacterium Belonging to the Roseobacter Clade
ABSTRACT Roseobacter strain 27-4 has been isolated from a turbot larval rearing unit and is capable of reducing mortality in turbot egg yolk sac larvae. Here, we demonstrate that the supernatant of Roseobacter 27-4 is lethal to the larval pathogens Vibrio anguillarum and Vibrio splendidus in a buffe...
Published in: | Applied and Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1128/aem.71.11.7263-7270.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.11.7263-7270.2005 |
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crasmicro:10.1128/aem.71.11.7263-7270.2005 2024-06-23T07:57:20+00:00 Ecology, Inhibitory Activity, and Morphogenesis of a Marine Antagonistic Bacterium Belonging to the Roseobacter Clade Bruhn, Jesper Bartholin Nielsen, Kristian Fog Hjelm, Mette Hansen, Michael Bresciani, José Schulz, Stefan Gram, Lone 2005 http://dx.doi.org/10.1128/aem.71.11.7263-7270.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.11.7263-7270.2005 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 71, issue 11, page 7263-7270 ISSN 0099-2240 1098-5336 journal-article 2005 crasmicro https://doi.org/10.1128/aem.71.11.7263-7270.2005 2024-06-10T04:07:34Z ABSTRACT Roseobacter strain 27-4 has been isolated from a turbot larval rearing unit and is capable of reducing mortality in turbot egg yolk sac larvae. Here, we demonstrate that the supernatant of Roseobacter 27-4 is lethal to the larval pathogens Vibrio anguillarum and Vibrio splendidus in a buffer system and inhibited their growth in marine broth. Liquid chromatography (LC) with both UV spectral detection and high-resolution mass spectrometry (HR-MS) identified the known antibacterial compound thiotropocin or its closely related precursor tropodithietic acid in the bioactive fractions. Antibacterial activity correlated with the appearance of a brownish pigment and was only formed in marine broth under static growth conditions. A thick biofilm of multicellular star-shaped aggregated cells formed at the air-liquid interface under static growth conditions. Here, the bioactive compound was the base peak in the LC-UV chromatograms of the extracts where it constituted 15% of the total peak area. Aerated conditions results in 10-fold-higher cell yield, however, cultures were nonpigmented, did not produce antibacterial activity, and grew as single cells. Production of antibacterial compounds may be quorum regulated, and we identified the acylated homoserine lactone (3-hydroxy-decanoyl homoserine lactone) from cultures of Roseobacter 27-4 using LC-HR-MS. The signal molecule was primarily detected in stagnant cultures. Roseobacter 27-4 grew between 10 and 30°C but died rapidly at 37°C. Also, the antibacterial compounds was sensitive to heat and was inactivated at 37°C in less than 2 days and at 25°C in 8 days. Using Roseobacter 27-4 as a probiotic culture will require that is be established in stagnant or adhered conditions and, due to the temperature sensitivity of the active compound, constant production must be ensured. Article in Journal/Newspaper Turbot ASM Journals (American Society for Microbiology) Base Peak ENVELOPE(-125.870,-125.870,55.283,55.283) Applied and Environmental Microbiology 71 11 7263 7270 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
description |
ABSTRACT Roseobacter strain 27-4 has been isolated from a turbot larval rearing unit and is capable of reducing mortality in turbot egg yolk sac larvae. Here, we demonstrate that the supernatant of Roseobacter 27-4 is lethal to the larval pathogens Vibrio anguillarum and Vibrio splendidus in a buffer system and inhibited their growth in marine broth. Liquid chromatography (LC) with both UV spectral detection and high-resolution mass spectrometry (HR-MS) identified the known antibacterial compound thiotropocin or its closely related precursor tropodithietic acid in the bioactive fractions. Antibacterial activity correlated with the appearance of a brownish pigment and was only formed in marine broth under static growth conditions. A thick biofilm of multicellular star-shaped aggregated cells formed at the air-liquid interface under static growth conditions. Here, the bioactive compound was the base peak in the LC-UV chromatograms of the extracts where it constituted 15% of the total peak area. Aerated conditions results in 10-fold-higher cell yield, however, cultures were nonpigmented, did not produce antibacterial activity, and grew as single cells. Production of antibacterial compounds may be quorum regulated, and we identified the acylated homoserine lactone (3-hydroxy-decanoyl homoserine lactone) from cultures of Roseobacter 27-4 using LC-HR-MS. The signal molecule was primarily detected in stagnant cultures. Roseobacter 27-4 grew between 10 and 30°C but died rapidly at 37°C. Also, the antibacterial compounds was sensitive to heat and was inactivated at 37°C in less than 2 days and at 25°C in 8 days. Using Roseobacter 27-4 as a probiotic culture will require that is be established in stagnant or adhered conditions and, due to the temperature sensitivity of the active compound, constant production must be ensured. |
format |
Article in Journal/Newspaper |
author |
Bruhn, Jesper Bartholin Nielsen, Kristian Fog Hjelm, Mette Hansen, Michael Bresciani, José Schulz, Stefan Gram, Lone |
spellingShingle |
Bruhn, Jesper Bartholin Nielsen, Kristian Fog Hjelm, Mette Hansen, Michael Bresciani, José Schulz, Stefan Gram, Lone Ecology, Inhibitory Activity, and Morphogenesis of a Marine Antagonistic Bacterium Belonging to the Roseobacter Clade |
author_facet |
Bruhn, Jesper Bartholin Nielsen, Kristian Fog Hjelm, Mette Hansen, Michael Bresciani, José Schulz, Stefan Gram, Lone |
author_sort |
Bruhn, Jesper Bartholin |
title |
Ecology, Inhibitory Activity, and Morphogenesis of a Marine Antagonistic Bacterium Belonging to the Roseobacter Clade |
title_short |
Ecology, Inhibitory Activity, and Morphogenesis of a Marine Antagonistic Bacterium Belonging to the Roseobacter Clade |
title_full |
Ecology, Inhibitory Activity, and Morphogenesis of a Marine Antagonistic Bacterium Belonging to the Roseobacter Clade |
title_fullStr |
Ecology, Inhibitory Activity, and Morphogenesis of a Marine Antagonistic Bacterium Belonging to the Roseobacter Clade |
title_full_unstemmed |
Ecology, Inhibitory Activity, and Morphogenesis of a Marine Antagonistic Bacterium Belonging to the Roseobacter Clade |
title_sort |
ecology, inhibitory activity, and morphogenesis of a marine antagonistic bacterium belonging to the roseobacter clade |
publisher |
American Society for Microbiology |
publishDate |
2005 |
url |
http://dx.doi.org/10.1128/aem.71.11.7263-7270.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.11.7263-7270.2005 |
long_lat |
ENVELOPE(-125.870,-125.870,55.283,55.283) |
geographic |
Base Peak |
geographic_facet |
Base Peak |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
Applied and Environmental Microbiology volume 71, issue 11, page 7263-7270 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.71.11.7263-7270.2005 |
container_title |
Applied and Environmental Microbiology |
container_volume |
71 |
container_issue |
11 |
container_start_page |
7263 |
op_container_end_page |
7270 |
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1802650920676229120 |