Putative Transposases Conserved in Exiguobacterium Isolates from Ancient Siberian Permafrost and from Contemporary Surface Habitats
ABSTRACT Gram-positive bacteria of the genus Exiguobacterium have been repeatedly isolated from Siberian permafrost ranging in age from 20,000 to 2 to 3 million years and have been sporadically recovered from markedly diverse habitats, including microbial mats in Lake Fryxell (Antarctic), surface wa...
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Online Access: | http://dx.doi.org/10.1128/aem.71.11.6954-6962.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.11.6954-6962.2005 |
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crasmicro:10.1128/aem.71.11.6954-6962.2005 2024-09-09T19:06:11+00:00 Putative Transposases Conserved in Exiguobacterium Isolates from Ancient Siberian Permafrost and from Contemporary Surface Habitats Vishnivetskaya, Tatiana A. Kathariou, Sophia 2005 http://dx.doi.org/10.1128/aem.71.11.6954-6962.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.11.6954-6962.2005 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 71, issue 11, page 6954-6962 ISSN 0099-2240 1098-5336 journal-article 2005 crasmicro https://doi.org/10.1128/aem.71.11.6954-6962.2005 2024-08-19T04:06:19Z ABSTRACT Gram-positive bacteria of the genus Exiguobacterium have been repeatedly isolated from Siberian permafrost ranging in age from 20,000 to 2 to 3 million years and have been sporadically recovered from markedly diverse habitats, including microbial mats in Lake Fryxell (Antarctic), surface water, and food-processing environments. However, there is currently no information on genomic diversity of this microorganism or on the physiological strategies that have allowed its survival under prolonged freezing in the permafrost. Analysis of the genome sequence of the most ancient available Exiguobacterium isolate ( Exiguobacterium sp. strain 255-15, from 2 to 3 million-year-old Siberian permafrost) revealed numerous putative transposase sequences, primarily of the IS 200 /IS 605 , IS 30 , and IS 3 families, with four transposase families identified. Several of the transposase genes appeared to be part of insertion sequences. Southern blots with different transposase probes yielded high-resolution genomic fingerprints which differentiated the different permafrost isolates from each other and from the Exiguobacterium spp. type strains which have been derived from diverse surface habitats. Each of the Exiguobacterium sp. strain 255-15 transposases that were used as probes had highly conserved homologs in the genome of other Exiguobacterium strains, both from permafrost and from modern sites. These findings suggest that, prior to their entrapment in permafrost, Exiguobacterium isolates had acquired transposases and that conserved transposases are present in Exiguobacterium spp., which now can be isolated from various modern surface habitats. Article in Journal/Newspaper Antarc* Antarctic permafrost ASM Journals (American Society for Microbiology) Antarctic Fryxell ENVELOPE(163.183,163.183,-77.617,-77.617) Lake Fryxell ENVELOPE(163.183,163.183,-77.617,-77.617) Applied and Environmental Microbiology 71 11 6954 6962 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT Gram-positive bacteria of the genus Exiguobacterium have been repeatedly isolated from Siberian permafrost ranging in age from 20,000 to 2 to 3 million years and have been sporadically recovered from markedly diverse habitats, including microbial mats in Lake Fryxell (Antarctic), surface water, and food-processing environments. However, there is currently no information on genomic diversity of this microorganism or on the physiological strategies that have allowed its survival under prolonged freezing in the permafrost. Analysis of the genome sequence of the most ancient available Exiguobacterium isolate ( Exiguobacterium sp. strain 255-15, from 2 to 3 million-year-old Siberian permafrost) revealed numerous putative transposase sequences, primarily of the IS 200 /IS 605 , IS 30 , and IS 3 families, with four transposase families identified. Several of the transposase genes appeared to be part of insertion sequences. Southern blots with different transposase probes yielded high-resolution genomic fingerprints which differentiated the different permafrost isolates from each other and from the Exiguobacterium spp. type strains which have been derived from diverse surface habitats. Each of the Exiguobacterium sp. strain 255-15 transposases that were used as probes had highly conserved homologs in the genome of other Exiguobacterium strains, both from permafrost and from modern sites. These findings suggest that, prior to their entrapment in permafrost, Exiguobacterium isolates had acquired transposases and that conserved transposases are present in Exiguobacterium spp., which now can be isolated from various modern surface habitats. |
format |
Article in Journal/Newspaper |
author |
Vishnivetskaya, Tatiana A. Kathariou, Sophia |
spellingShingle |
Vishnivetskaya, Tatiana A. Kathariou, Sophia Putative Transposases Conserved in Exiguobacterium Isolates from Ancient Siberian Permafrost and from Contemporary Surface Habitats |
author_facet |
Vishnivetskaya, Tatiana A. Kathariou, Sophia |
author_sort |
Vishnivetskaya, Tatiana A. |
title |
Putative Transposases Conserved in Exiguobacterium Isolates from Ancient Siberian Permafrost and from Contemporary Surface Habitats |
title_short |
Putative Transposases Conserved in Exiguobacterium Isolates from Ancient Siberian Permafrost and from Contemporary Surface Habitats |
title_full |
Putative Transposases Conserved in Exiguobacterium Isolates from Ancient Siberian Permafrost and from Contemporary Surface Habitats |
title_fullStr |
Putative Transposases Conserved in Exiguobacterium Isolates from Ancient Siberian Permafrost and from Contemporary Surface Habitats |
title_full_unstemmed |
Putative Transposases Conserved in Exiguobacterium Isolates from Ancient Siberian Permafrost and from Contemporary Surface Habitats |
title_sort |
putative transposases conserved in exiguobacterium isolates from ancient siberian permafrost and from contemporary surface habitats |
publisher |
American Society for Microbiology |
publishDate |
2005 |
url |
http://dx.doi.org/10.1128/aem.71.11.6954-6962.2005 https://journals.asm.org/doi/pdf/10.1128/AEM.71.11.6954-6962.2005 |
long_lat |
ENVELOPE(163.183,163.183,-77.617,-77.617) ENVELOPE(163.183,163.183,-77.617,-77.617) |
geographic |
Antarctic Fryxell Lake Fryxell |
geographic_facet |
Antarctic Fryxell Lake Fryxell |
genre |
Antarc* Antarctic permafrost |
genre_facet |
Antarc* Antarctic permafrost |
op_source |
Applied and Environmental Microbiology volume 71, issue 11, page 6954-6962 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.71.11.6954-6962.2005 |
container_title |
Applied and Environmental Microbiology |
container_volume |
71 |
container_issue |
11 |
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6954 |
op_container_end_page |
6962 |
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1809820229031690240 |