Combining Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization and Microautoradiography To Detect Substrate Utilization by Bacteria and Archaea in the Deep Ocean

• Published in: Applied and Environmental Microbiology
• Main Authors: Teira, Eva, Reinthaler, Thomas, Pernthaler, Annelie, Pernthaler, Jakob, Herndl, Gerhard J.
• Format: Article in Journal/Newspaper
• Language: English
• Published: American Society for Microbiology 2004
• Subjects: North Atlantic
• Online Access: http://dx.doi.org/10.1128/aem.70.7.4411-4414.2004; https://journals.asm.org/doi/pdf/10.1128/AEM.70.7.4411-4414.2004

ABSTRACT The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resulted in about twofold-higher detection rates for Archaea in deep waters. Using this method in combination with microautoradiography, we found that Archaea are more abundant than Bacteria (42% versus 32% of 4′,6′-diamidino-2-phenylindole counts) in the deep waters of the North Atlantic and that a larger fraction of Archaea than of Bacteria takes up l -aspartic acid (19% versus 10%).