Analysis of Dissimilatory Sulfite Reductase and 16S rRNA Gene Fragments from Deep-Sea Hydrothermal Sites of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific
ABSTRACT This study describes the occurrence of unique dissimilatory sulfite reductase (DSR) genes at a depth of 1,380 m from the deep-sea hydrothermal vent field at the Suiyo Seamount, Izu-Bonin Arc, Western Pacific, Japan. The DSR genes were obtained from microbes that grew in a catheter-type in s...
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Online Access: | http://dx.doi.org/10.1128/aem.70.1.393-403.2004 https://journals.asm.org/doi/pdf/10.1128/AEM.70.1.393-403.2004 |
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crasmicro:10.1128/aem.70.1.393-403.2004 2024-06-23T07:50:40+00:00 Analysis of Dissimilatory Sulfite Reductase and 16S rRNA Gene Fragments from Deep-Sea Hydrothermal Sites of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific Nakagawa, Tatsunori Ishibashi, Jun-Ichiro Maruyama, Akihiko Yamanaka, Toshiro Morimoto, Yusuke Kimura, Hiroyuki Urabe, Tetsuro Fukui, Manabu 2004 http://dx.doi.org/10.1128/aem.70.1.393-403.2004 https://journals.asm.org/doi/pdf/10.1128/AEM.70.1.393-403.2004 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 70, issue 1, page 393-403 ISSN 0099-2240 1098-5336 journal-article 2004 crasmicro https://doi.org/10.1128/aem.70.1.393-403.2004 2024-06-03T08:10:55Z ABSTRACT This study describes the occurrence of unique dissimilatory sulfite reductase (DSR) genes at a depth of 1,380 m from the deep-sea hydrothermal vent field at the Suiyo Seamount, Izu-Bonin Arc, Western Pacific, Japan. The DSR genes were obtained from microbes that grew in a catheter-type in situ growth chamber deployed for 3 days on a vent and from the effluent water of drilled holes at 5°C and natural vent fluids at 7°C. DSR clones SUIYOdsr-A and SUIYOdsr-B were not closely related to cultivated species or environmental clones. Moreover, samples of microbial communities were examined by PCR-denaturing gradient gel electrophoresis (DGGE) analysis of the 16S rRNA gene. The sequence analysis of 16S rRNA gene fragments obtained from the vent catheter after a 3-day incubation revealed the occurrence of bacterial DGGE bands affiliated with the Aquificae and γ- and ε- Proteobacteria as well as the occurrence of archaeal phylotypes affiliated with the Thermococcales and of a unique archaeon sequence that clustered with “ Nanoarchaeota .” The DGGE bands obtained from drilled holes and natural vent fluids from 7 to 300°C were affiliated with the δ- Proteobacteria , genus Thiomicrospira , and Pelodictyon . The dominant DGGE bands retrieved from the effluent water of casing pipes at 3 and 4°C were closely related to phylotypes obtained from the Arctic Ocean. Our results suggest the presence of microorganisms corresponding to a unique DSR lineage not detected previously from other geothermal environments. Article in Journal/Newspaper Arctic Arctic Ocean ASM Journals (American Society for Microbiology) Arctic Arctic Ocean Pacific Applied and Environmental Microbiology 70 1 393 403 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT This study describes the occurrence of unique dissimilatory sulfite reductase (DSR) genes at a depth of 1,380 m from the deep-sea hydrothermal vent field at the Suiyo Seamount, Izu-Bonin Arc, Western Pacific, Japan. The DSR genes were obtained from microbes that grew in a catheter-type in situ growth chamber deployed for 3 days on a vent and from the effluent water of drilled holes at 5°C and natural vent fluids at 7°C. DSR clones SUIYOdsr-A and SUIYOdsr-B were not closely related to cultivated species or environmental clones. Moreover, samples of microbial communities were examined by PCR-denaturing gradient gel electrophoresis (DGGE) analysis of the 16S rRNA gene. The sequence analysis of 16S rRNA gene fragments obtained from the vent catheter after a 3-day incubation revealed the occurrence of bacterial DGGE bands affiliated with the Aquificae and γ- and ε- Proteobacteria as well as the occurrence of archaeal phylotypes affiliated with the Thermococcales and of a unique archaeon sequence that clustered with “ Nanoarchaeota .” The DGGE bands obtained from drilled holes and natural vent fluids from 7 to 300°C were affiliated with the δ- Proteobacteria , genus Thiomicrospira , and Pelodictyon . The dominant DGGE bands retrieved from the effluent water of casing pipes at 3 and 4°C were closely related to phylotypes obtained from the Arctic Ocean. Our results suggest the presence of microorganisms corresponding to a unique DSR lineage not detected previously from other geothermal environments. |
format |
Article in Journal/Newspaper |
author |
Nakagawa, Tatsunori Ishibashi, Jun-Ichiro Maruyama, Akihiko Yamanaka, Toshiro Morimoto, Yusuke Kimura, Hiroyuki Urabe, Tetsuro Fukui, Manabu |
spellingShingle |
Nakagawa, Tatsunori Ishibashi, Jun-Ichiro Maruyama, Akihiko Yamanaka, Toshiro Morimoto, Yusuke Kimura, Hiroyuki Urabe, Tetsuro Fukui, Manabu Analysis of Dissimilatory Sulfite Reductase and 16S rRNA Gene Fragments from Deep-Sea Hydrothermal Sites of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific |
author_facet |
Nakagawa, Tatsunori Ishibashi, Jun-Ichiro Maruyama, Akihiko Yamanaka, Toshiro Morimoto, Yusuke Kimura, Hiroyuki Urabe, Tetsuro Fukui, Manabu |
author_sort |
Nakagawa, Tatsunori |
title |
Analysis of Dissimilatory Sulfite Reductase and 16S rRNA Gene Fragments from Deep-Sea Hydrothermal Sites of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific |
title_short |
Analysis of Dissimilatory Sulfite Reductase and 16S rRNA Gene Fragments from Deep-Sea Hydrothermal Sites of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific |
title_full |
Analysis of Dissimilatory Sulfite Reductase and 16S rRNA Gene Fragments from Deep-Sea Hydrothermal Sites of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific |
title_fullStr |
Analysis of Dissimilatory Sulfite Reductase and 16S rRNA Gene Fragments from Deep-Sea Hydrothermal Sites of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific |
title_full_unstemmed |
Analysis of Dissimilatory Sulfite Reductase and 16S rRNA Gene Fragments from Deep-Sea Hydrothermal Sites of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific |
title_sort |
analysis of dissimilatory sulfite reductase and 16s rrna gene fragments from deep-sea hydrothermal sites of the suiyo seamount, izu-bonin arc, western pacific |
publisher |
American Society for Microbiology |
publishDate |
2004 |
url |
http://dx.doi.org/10.1128/aem.70.1.393-403.2004 https://journals.asm.org/doi/pdf/10.1128/AEM.70.1.393-403.2004 |
geographic |
Arctic Arctic Ocean Pacific |
geographic_facet |
Arctic Arctic Ocean Pacific |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_source |
Applied and Environmental Microbiology volume 70, issue 1, page 393-403 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.70.1.393-403.2004 |
container_title |
Applied and Environmental Microbiology |
container_volume |
70 |
container_issue |
1 |
container_start_page |
393 |
op_container_end_page |
403 |
_version_ |
1802641584194322432 |