Cyanobacterial Diversity in Natural and Artificial Microbial Mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): a Morphological and Molecular Approach
ABSTRACT Currently, there is no consensus concerning the geographic distribution and extent of endemism in Antarctic cyanobacteria. In this paper we describe the phenotypic and genotypic diversity of cyanobacteria in a field microbial mat sample from Lake Fryxell and in an artificial cold-adapted sa...
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Online Access: | http://dx.doi.org/10.1128/aem.69.9.5157-5169.2003 https://journals.asm.org/doi/pdf/10.1128/AEM.69.9.5157-5169.2003 |
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crasmicro:10.1128/aem.69.9.5157-5169.2003 2024-06-23T07:47:06+00:00 Cyanobacterial Diversity in Natural and Artificial Microbial Mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): a Morphological and Molecular Approach Taton, Arnaud Grubisic, Stana Brambilla, Evelyne De Wit, Rutger Wilmotte, Annick 2003 http://dx.doi.org/10.1128/aem.69.9.5157-5169.2003 https://journals.asm.org/doi/pdf/10.1128/AEM.69.9.5157-5169.2003 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 69, issue 9, page 5157-5169 ISSN 0099-2240 1098-5336 journal-article 2003 crasmicro https://doi.org/10.1128/aem.69.9.5157-5169.2003 2024-06-10T04:07:25Z ABSTRACT Currently, there is no consensus concerning the geographic distribution and extent of endemism in Antarctic cyanobacteria. In this paper we describe the phenotypic and genotypic diversity of cyanobacteria in a field microbial mat sample from Lake Fryxell and in an artificial cold-adapted sample cultured in a benthic gradient chamber (BGC) by using an inoculum from the same mat. Light microscopy and molecular tools, including 16S rRNA gene clone libraries, denaturing gradient gel electrophoresis, and sequencing, were used. For the first time in the study of cyanobacterial diversity of environmental samples, internal transcribed spacer (ITS) sequences were retrieved and analyzed to complement the information obtained from the 16S rRNA gene. Microscopy allowed eight morphotypes to be identified, only one of which is likely to be an Antarctic endemic morphotype. Molecular analysis, however, revealed an entirely different pattern. A much higher number of phylotypes (15 phylotypes) was found, but no sequences from Nodularia and Hydrocoryne , as observed by microscopy, were retrieved. The 16S rRNA gene sequences determined in this study were distributed in 11 phylogenetic lineages, 3 of which were exclusively Antarctic and 2 of which were novel. Collectively, these Antarctic sequences together with all the other polar sequences were distributed in 22 lineages, 9 of which were exclusively Antarctic, including the 2 novel lineages observed in this study. The cultured BGC mat had lower diversity than the field mat. However, the two samples shared three morphotypes and three phylotypes. Moreover, the BGC mat allowed enrichment of one additional phylotype. ITS sequence analysis revealed a complex signal that was difficult to interpret. Finally, this study provided evidence of molecular diversity of cyanobacteria in Antarctica that is much greater than the diversity currently known based on traditional microscopic analysis. Furthermore, Antarctic endemic species were more abundant than was estimated on the basis of ... Article in Journal/Newspaper Antarc* Antarctic Antarctica McMurdo Dry Valleys ASM Journals (American Society for Microbiology) Antarctic Fryxell ENVELOPE(163.183,163.183,-77.617,-77.617) Lake Fryxell ENVELOPE(163.183,163.183,-77.617,-77.617) McMurdo Dry Valleys Applied and Environmental Microbiology 69 9 5157 5169 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT Currently, there is no consensus concerning the geographic distribution and extent of endemism in Antarctic cyanobacteria. In this paper we describe the phenotypic and genotypic diversity of cyanobacteria in a field microbial mat sample from Lake Fryxell and in an artificial cold-adapted sample cultured in a benthic gradient chamber (BGC) by using an inoculum from the same mat. Light microscopy and molecular tools, including 16S rRNA gene clone libraries, denaturing gradient gel electrophoresis, and sequencing, were used. For the first time in the study of cyanobacterial diversity of environmental samples, internal transcribed spacer (ITS) sequences were retrieved and analyzed to complement the information obtained from the 16S rRNA gene. Microscopy allowed eight morphotypes to be identified, only one of which is likely to be an Antarctic endemic morphotype. Molecular analysis, however, revealed an entirely different pattern. A much higher number of phylotypes (15 phylotypes) was found, but no sequences from Nodularia and Hydrocoryne , as observed by microscopy, were retrieved. The 16S rRNA gene sequences determined in this study were distributed in 11 phylogenetic lineages, 3 of which were exclusively Antarctic and 2 of which were novel. Collectively, these Antarctic sequences together with all the other polar sequences were distributed in 22 lineages, 9 of which were exclusively Antarctic, including the 2 novel lineages observed in this study. The cultured BGC mat had lower diversity than the field mat. However, the two samples shared three morphotypes and three phylotypes. Moreover, the BGC mat allowed enrichment of one additional phylotype. ITS sequence analysis revealed a complex signal that was difficult to interpret. Finally, this study provided evidence of molecular diversity of cyanobacteria in Antarctica that is much greater than the diversity currently known based on traditional microscopic analysis. Furthermore, Antarctic endemic species were more abundant than was estimated on the basis of ... |
format |
Article in Journal/Newspaper |
author |
Taton, Arnaud Grubisic, Stana Brambilla, Evelyne De Wit, Rutger Wilmotte, Annick |
spellingShingle |
Taton, Arnaud Grubisic, Stana Brambilla, Evelyne De Wit, Rutger Wilmotte, Annick Cyanobacterial Diversity in Natural and Artificial Microbial Mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): a Morphological and Molecular Approach |
author_facet |
Taton, Arnaud Grubisic, Stana Brambilla, Evelyne De Wit, Rutger Wilmotte, Annick |
author_sort |
Taton, Arnaud |
title |
Cyanobacterial Diversity in Natural and Artificial Microbial Mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): a Morphological and Molecular Approach |
title_short |
Cyanobacterial Diversity in Natural and Artificial Microbial Mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): a Morphological and Molecular Approach |
title_full |
Cyanobacterial Diversity in Natural and Artificial Microbial Mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): a Morphological and Molecular Approach |
title_fullStr |
Cyanobacterial Diversity in Natural and Artificial Microbial Mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): a Morphological and Molecular Approach |
title_full_unstemmed |
Cyanobacterial Diversity in Natural and Artificial Microbial Mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): a Morphological and Molecular Approach |
title_sort |
cyanobacterial diversity in natural and artificial microbial mats of lake fryxell (mcmurdo dry valleys, antarctica): a morphological and molecular approach |
publisher |
American Society for Microbiology |
publishDate |
2003 |
url |
http://dx.doi.org/10.1128/aem.69.9.5157-5169.2003 https://journals.asm.org/doi/pdf/10.1128/AEM.69.9.5157-5169.2003 |
long_lat |
ENVELOPE(163.183,163.183,-77.617,-77.617) ENVELOPE(163.183,163.183,-77.617,-77.617) |
geographic |
Antarctic Fryxell Lake Fryxell McMurdo Dry Valleys |
geographic_facet |
Antarctic Fryxell Lake Fryxell McMurdo Dry Valleys |
genre |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
genre_facet |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
op_source |
Applied and Environmental Microbiology volume 69, issue 9, page 5157-5169 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.69.9.5157-5169.2003 |
container_title |
Applied and Environmental Microbiology |
container_volume |
69 |
container_issue |
9 |
container_start_page |
5157 |
op_container_end_page |
5169 |
_version_ |
1802650939697397760 |