Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil
ABSTRACT Seventy-five aerobic heterotrophs have been isolated from a packed-column bioreactor inoculated with soil from Antarctica. The column was maintained at 10°C and continuously fed with a casein-containing medium to enrich protease producers. Twenty-eight isolates were selected for further cha...
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Online Access: | http://dx.doi.org/10.1128/aem.69.3.1457-1464.2003 https://journals.asm.org/doi/pdf/10.1128/AEM.69.3.1457-1464.2003 |
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crasmicro:10.1128/aem.69.3.1457-1464.2003 2024-06-23T07:47:48+00:00 Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil Wery, Nathalie Gerike, Ursula Sharman, Ajay Chaudhuri, Julian B. Hough, David W. Danson, Michael J. 2003 http://dx.doi.org/10.1128/aem.69.3.1457-1464.2003 https://journals.asm.org/doi/pdf/10.1128/AEM.69.3.1457-1464.2003 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 69, issue 3, page 1457-1464 ISSN 0099-2240 1098-5336 journal-article 2003 crasmicro https://doi.org/10.1128/aem.69.3.1457-1464.2003 2024-05-27T12:59:34Z ABSTRACT Seventy-five aerobic heterotrophs have been isolated from a packed-column bioreactor inoculated with soil from Antarctica. The column was maintained at 10°C and continuously fed with a casein-containing medium to enrich protease producers. Twenty-eight isolates were selected for further characterization on the basis of morphology and production of clearing zones on skim milk plates. Phenotypic tests indicated that the strains were mainly psychrotrophs and presented a high morphological and metabolical diversity. The extracellular protease activities tested were optimal at neutral pH and between 30 and 45°C. 16S ribosomal DNA sequence analyses showed that the bioreactor was colonized by a wide variety of taxons, belonging to various bacterial divisions: α-, β-, and γ- Proteobacteria the Flexibacter - Cytophaga - Bacteroides group; and high G+C gram-positive bacteria and low G+C gram-positive bacteria. Some strains represent candidates for new species of the genera Chryseobacterium and Massilia . This diversity demonstrates that the bioreactor is an efficient enrichment tool compared to traditional isolation strategies. Article in Journal/Newspaper Antarc* Antarctic Antarctica ASM Journals (American Society for Microbiology) Antarctic Applied and Environmental Microbiology 69 3 1457 1464 |
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Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
description |
ABSTRACT Seventy-five aerobic heterotrophs have been isolated from a packed-column bioreactor inoculated with soil from Antarctica. The column was maintained at 10°C and continuously fed with a casein-containing medium to enrich protease producers. Twenty-eight isolates were selected for further characterization on the basis of morphology and production of clearing zones on skim milk plates. Phenotypic tests indicated that the strains were mainly psychrotrophs and presented a high morphological and metabolical diversity. The extracellular protease activities tested were optimal at neutral pH and between 30 and 45°C. 16S ribosomal DNA sequence analyses showed that the bioreactor was colonized by a wide variety of taxons, belonging to various bacterial divisions: α-, β-, and γ- Proteobacteria the Flexibacter - Cytophaga - Bacteroides group; and high G+C gram-positive bacteria and low G+C gram-positive bacteria. Some strains represent candidates for new species of the genera Chryseobacterium and Massilia . This diversity demonstrates that the bioreactor is an efficient enrichment tool compared to traditional isolation strategies. |
format |
Article in Journal/Newspaper |
author |
Wery, Nathalie Gerike, Ursula Sharman, Ajay Chaudhuri, Julian B. Hough, David W. Danson, Michael J. |
spellingShingle |
Wery, Nathalie Gerike, Ursula Sharman, Ajay Chaudhuri, Julian B. Hough, David W. Danson, Michael J. Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil |
author_facet |
Wery, Nathalie Gerike, Ursula Sharman, Ajay Chaudhuri, Julian B. Hough, David W. Danson, Michael J. |
author_sort |
Wery, Nathalie |
title |
Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil |
title_short |
Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil |
title_full |
Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil |
title_fullStr |
Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil |
title_full_unstemmed |
Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil |
title_sort |
use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil |
publisher |
American Society for Microbiology |
publishDate |
2003 |
url |
http://dx.doi.org/10.1128/aem.69.3.1457-1464.2003 https://journals.asm.org/doi/pdf/10.1128/AEM.69.3.1457-1464.2003 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Applied and Environmental Microbiology volume 69, issue 3, page 1457-1464 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.69.3.1457-1464.2003 |
container_title |
Applied and Environmental Microbiology |
container_volume |
69 |
container_issue |
3 |
container_start_page |
1457 |
op_container_end_page |
1464 |
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1802637968988438528 |