Unveiling the Organisms behind Novel Eukaryotic Ribosomal DNA Sequences from the Ocean
ABSTRACT Despite the fact that the smallest eukaryotes (cells less than 5 μm in diameter) play key roles in marine food webs, particularly in open oligotrophic areas, the study of their in situ diversity started just one year ago. Perhaps the most remarkable finding of the most recent studies has be...
Published in: | Applied and Environmental Microbiology |
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American Society for Microbiology
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Online Access: | http://dx.doi.org/10.1128/aem.68.9.4554-4558.2002 https://journals.asm.org/doi/pdf/10.1128/AEM.68.9.4554-4558.2002 |
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crasmicro:10.1128/aem.68.9.4554-4558.2002 2024-06-23T07:45:50+00:00 Unveiling the Organisms behind Novel Eukaryotic Ribosomal DNA Sequences from the Ocean Massana, Ramon Guillou, Laure Díez, Beatriz Pedrós-Alió, Carlos 2002 http://dx.doi.org/10.1128/aem.68.9.4554-4558.2002 https://journals.asm.org/doi/pdf/10.1128/AEM.68.9.4554-4558.2002 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 68, issue 9, page 4554-4558 ISSN 0099-2240 1098-5336 journal-article 2002 crasmicro https://doi.org/10.1128/aem.68.9.4554-4558.2002 2024-06-10T04:07:24Z ABSTRACT Despite the fact that the smallest eukaryotes (cells less than 5 μm in diameter) play key roles in marine food webs, particularly in open oligotrophic areas, the study of their in situ diversity started just one year ago. Perhaps the most remarkable finding of the most recent studies has been the discovery of completely new phylogenetic lineages, such as novel clades belonging to the stramenopile and alveolate phyla. The two new groups account for a significant fraction of clones in genetic libraries from North Atlantic, equatorial Pacific, Antarctic, and Mediterranean Sea waters. However, the identities and ecological relevance of these organisms remain unknown. Here we investigate the phylogenetic relationships, morphology, in situ abundance, and ecological role of novel stramenopiles. They form at least eight independent clades within the stramenopile basal branches, indicating a large phylogenetic diversity within the group. Two lineages were visualized and enumerated in field samples and enrichments by fluorescent in situ hybridization using specific rRNA-targeted oligonucleotide probes. The targeted organisms were 2- to 3-μm-diameter, round-shaped, nonpigmented flagellates. Further, they were found to be bacterivorous. One lineage accounted for up to 46% (average during an annual cycle, 19%) of heterotrophic flagellates in a coastal environment, providing evidence that novel stramenopiles are important and unrecognized components of the total stock of bacterial grazers. Article in Journal/Newspaper Antarc* Antarctic North Atlantic ASM Journals (American Society for Microbiology) Antarctic Pacific Applied and Environmental Microbiology 68 9 4554 4558 |
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Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT Despite the fact that the smallest eukaryotes (cells less than 5 μm in diameter) play key roles in marine food webs, particularly in open oligotrophic areas, the study of their in situ diversity started just one year ago. Perhaps the most remarkable finding of the most recent studies has been the discovery of completely new phylogenetic lineages, such as novel clades belonging to the stramenopile and alveolate phyla. The two new groups account for a significant fraction of clones in genetic libraries from North Atlantic, equatorial Pacific, Antarctic, and Mediterranean Sea waters. However, the identities and ecological relevance of these organisms remain unknown. Here we investigate the phylogenetic relationships, morphology, in situ abundance, and ecological role of novel stramenopiles. They form at least eight independent clades within the stramenopile basal branches, indicating a large phylogenetic diversity within the group. Two lineages were visualized and enumerated in field samples and enrichments by fluorescent in situ hybridization using specific rRNA-targeted oligonucleotide probes. The targeted organisms were 2- to 3-μm-diameter, round-shaped, nonpigmented flagellates. Further, they were found to be bacterivorous. One lineage accounted for up to 46% (average during an annual cycle, 19%) of heterotrophic flagellates in a coastal environment, providing evidence that novel stramenopiles are important and unrecognized components of the total stock of bacterial grazers. |
format |
Article in Journal/Newspaper |
author |
Massana, Ramon Guillou, Laure Díez, Beatriz Pedrós-Alió, Carlos |
spellingShingle |
Massana, Ramon Guillou, Laure Díez, Beatriz Pedrós-Alió, Carlos Unveiling the Organisms behind Novel Eukaryotic Ribosomal DNA Sequences from the Ocean |
author_facet |
Massana, Ramon Guillou, Laure Díez, Beatriz Pedrós-Alió, Carlos |
author_sort |
Massana, Ramon |
title |
Unveiling the Organisms behind Novel Eukaryotic Ribosomal DNA Sequences from the Ocean |
title_short |
Unveiling the Organisms behind Novel Eukaryotic Ribosomal DNA Sequences from the Ocean |
title_full |
Unveiling the Organisms behind Novel Eukaryotic Ribosomal DNA Sequences from the Ocean |
title_fullStr |
Unveiling the Organisms behind Novel Eukaryotic Ribosomal DNA Sequences from the Ocean |
title_full_unstemmed |
Unveiling the Organisms behind Novel Eukaryotic Ribosomal DNA Sequences from the Ocean |
title_sort |
unveiling the organisms behind novel eukaryotic ribosomal dna sequences from the ocean |
publisher |
American Society for Microbiology |
publishDate |
2002 |
url |
http://dx.doi.org/10.1128/aem.68.9.4554-4558.2002 https://journals.asm.org/doi/pdf/10.1128/AEM.68.9.4554-4558.2002 |
geographic |
Antarctic Pacific |
geographic_facet |
Antarctic Pacific |
genre |
Antarc* Antarctic North Atlantic |
genre_facet |
Antarc* Antarctic North Atlantic |
op_source |
Applied and Environmental Microbiology volume 68, issue 9, page 4554-4558 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.68.9.4554-4558.2002 |
container_title |
Applied and Environmental Microbiology |
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68 |
container_issue |
9 |
container_start_page |
4554 |
op_container_end_page |
4558 |
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1802642602769514496 |