Microbial Communities from Methane Hydrate-Bearing Deep Marine Sediments in a Forearc Basin
ABSTRACT Microbial communities in cores obtained from methane hydrate-bearing deep marine sediments (down to more than 300 m below the seafloor) in the forearc basin of the Nankai Trough near Japan were characterized with cultivation-dependent and -independent techniques. Acridine orange direct coun...
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Online Access: | http://dx.doi.org/10.1128/aem.68.8.3759-3770.2002 https://journals.asm.org/doi/pdf/10.1128/AEM.68.8.3759-3770.2002 |
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crasmicro:10.1128/aem.68.8.3759-3770.2002 2024-09-15T18:18:37+00:00 Microbial Communities from Methane Hydrate-Bearing Deep Marine Sediments in a Forearc Basin Reed, David W. Fujita, Yoshiko Delwiche, Mark E. Blackwelder, D. Brad Sheridan, Peter P. Uchida, Takashi Colwell, Frederick S. 2002 http://dx.doi.org/10.1128/aem.68.8.3759-3770.2002 https://journals.asm.org/doi/pdf/10.1128/AEM.68.8.3759-3770.2002 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 68, issue 8, page 3759-3770 ISSN 0099-2240 1098-5336 journal-article 2002 crasmicro https://doi.org/10.1128/aem.68.8.3759-3770.2002 2024-08-12T04:06:05Z ABSTRACT Microbial communities in cores obtained from methane hydrate-bearing deep marine sediments (down to more than 300 m below the seafloor) in the forearc basin of the Nankai Trough near Japan were characterized with cultivation-dependent and -independent techniques. Acridine orange direct count data indicated that cell numbers generally decreased with sediment depth. Lipid biomarker analyses indicated the presence of viable biomass at concentrations greater than previously reported for terrestrial subsurface environments at similar depths. Archaeal lipids were more abundant than bacterial lipids. Methane was produced from both acetate and hydrogen in enrichments inoculated with sediment from all depths evaluated, at both 10 and 35°C. Characterization of 16S rRNA genes amplified from the sediments indicated that archaeal clones could be discretely grouped within the Euryarchaeota and Crenarchaeota domains. The bacterial clones exhibited greater overall diversity than the archaeal clones, with sequences related to the Bacteroidetes , Planctomycetes , Actinobacteria , Proteobacteria , and green nonsulfur groups. The majority of the bacterial clones were either members of a novel lineage or most closely related to uncultured clones. The results of these analyses suggest that the microbial community in this environment is distinct from those in previously characterized methane hydrate-bearing sediments. Article in Journal/Newspaper Methane hydrate ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 68 8 3759 3770 |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT Microbial communities in cores obtained from methane hydrate-bearing deep marine sediments (down to more than 300 m below the seafloor) in the forearc basin of the Nankai Trough near Japan were characterized with cultivation-dependent and -independent techniques. Acridine orange direct count data indicated that cell numbers generally decreased with sediment depth. Lipid biomarker analyses indicated the presence of viable biomass at concentrations greater than previously reported for terrestrial subsurface environments at similar depths. Archaeal lipids were more abundant than bacterial lipids. Methane was produced from both acetate and hydrogen in enrichments inoculated with sediment from all depths evaluated, at both 10 and 35°C. Characterization of 16S rRNA genes amplified from the sediments indicated that archaeal clones could be discretely grouped within the Euryarchaeota and Crenarchaeota domains. The bacterial clones exhibited greater overall diversity than the archaeal clones, with sequences related to the Bacteroidetes , Planctomycetes , Actinobacteria , Proteobacteria , and green nonsulfur groups. The majority of the bacterial clones were either members of a novel lineage or most closely related to uncultured clones. The results of these analyses suggest that the microbial community in this environment is distinct from those in previously characterized methane hydrate-bearing sediments. |
format |
Article in Journal/Newspaper |
author |
Reed, David W. Fujita, Yoshiko Delwiche, Mark E. Blackwelder, D. Brad Sheridan, Peter P. Uchida, Takashi Colwell, Frederick S. |
spellingShingle |
Reed, David W. Fujita, Yoshiko Delwiche, Mark E. Blackwelder, D. Brad Sheridan, Peter P. Uchida, Takashi Colwell, Frederick S. Microbial Communities from Methane Hydrate-Bearing Deep Marine Sediments in a Forearc Basin |
author_facet |
Reed, David W. Fujita, Yoshiko Delwiche, Mark E. Blackwelder, D. Brad Sheridan, Peter P. Uchida, Takashi Colwell, Frederick S. |
author_sort |
Reed, David W. |
title |
Microbial Communities from Methane Hydrate-Bearing Deep Marine Sediments in a Forearc Basin |
title_short |
Microbial Communities from Methane Hydrate-Bearing Deep Marine Sediments in a Forearc Basin |
title_full |
Microbial Communities from Methane Hydrate-Bearing Deep Marine Sediments in a Forearc Basin |
title_fullStr |
Microbial Communities from Methane Hydrate-Bearing Deep Marine Sediments in a Forearc Basin |
title_full_unstemmed |
Microbial Communities from Methane Hydrate-Bearing Deep Marine Sediments in a Forearc Basin |
title_sort |
microbial communities from methane hydrate-bearing deep marine sediments in a forearc basin |
publisher |
American Society for Microbiology |
publishDate |
2002 |
url |
http://dx.doi.org/10.1128/aem.68.8.3759-3770.2002 https://journals.asm.org/doi/pdf/10.1128/AEM.68.8.3759-3770.2002 |
genre |
Methane hydrate |
genre_facet |
Methane hydrate |
op_source |
Applied and Environmental Microbiology volume 68, issue 8, page 3759-3770 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.68.8.3759-3770.2002 |
container_title |
Applied and Environmental Microbiology |
container_volume |
68 |
container_issue |
8 |
container_start_page |
3759 |
op_container_end_page |
3770 |
_version_ |
1810456720754868224 |