Use of Fe(III) as an Electron Acceptor To Recover Previously Uncultured Hyperthermophiles: Isolation and Characterization of Geothermobacterium ferrireducens gen. nov., sp. nov
ABSTRACT It has recently been recognized that the ability to use Fe(III) as a terminal electron acceptor is a highly conserved characteristic in hyperthermophilic microorganisms. This suggests that it may be possible to recover as-yet-uncultured hyperthermophiles in pure culture if Fe(III) is used a...
Published in: | Applied and Environmental Microbiology |
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American Society for Microbiology
2002
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Online Access: | http://dx.doi.org/10.1128/aem.68.4.1735-1742.2002 https://journals.asm.org/doi/pdf/10.1128/AEM.68.4.1735-1742.2002 |
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crasmicro:10.1128/aem.68.4.1735-1742.2002 2024-09-30T14:37:36+00:00 Use of Fe(III) as an Electron Acceptor To Recover Previously Uncultured Hyperthermophiles: Isolation and Characterization of Geothermobacterium ferrireducens gen. nov., sp. nov Kashefi, Kazem Holmes, Dawn E. Reysenbach, Anna-Louise Lovley, Derek R. 2002 http://dx.doi.org/10.1128/aem.68.4.1735-1742.2002 https://journals.asm.org/doi/pdf/10.1128/AEM.68.4.1735-1742.2002 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 68, issue 4, page 1735-1742 ISSN 0099-2240 1098-5336 journal-article 2002 crasmicro https://doi.org/10.1128/aem.68.4.1735-1742.2002 2024-09-17T04:08:06Z ABSTRACT It has recently been recognized that the ability to use Fe(III) as a terminal electron acceptor is a highly conserved characteristic in hyperthermophilic microorganisms. This suggests that it may be possible to recover as-yet-uncultured hyperthermophiles in pure culture if Fe(III) is used as an electron acceptor. As part of a study of the microbial diversity of the Obsidian Pool area in Yellowstone National Park, Wyo., hot sediment samples were used as the inoculum for enrichment cultures in media containing hydrogen as the sole electron donor and poorly crystalline Fe(III) oxide as the electron acceptor. A pure culture was recovered on solidified, Fe(III) oxide medium. The isolate, designated FW-1a, is a hyperthermophilic anaerobe that grows exclusively by coupling hydrogen oxidation to the reduction of poorly crystalline Fe(III) oxide. Organic carbon is not required for growth. Magnetite is the end product of Fe(III) oxide reduction under the culture conditions evaluated. The cells are rod shaped, about 0.5 μm by 1.0 to 1.2 μm, and motile and have a single flagellum. Strain FW-1a grows at circumneutral pH, at freshwater salinities, and at temperatures of between 65 and 100°C with an optimum of 85 to 90°C. To our knowledge this is the highest temperature optimum of any organism in the Bacteria . Analysis of the 16S ribosomal DNA (rDNA) sequence of strain FW-1a places it within the Bacteria , most closely related to abundant but uncultured microorganisms whose 16S rDNA sequences have been previously recovered from Obsidian Pool and a terrestrial hot spring in Iceland. While previous studies inferred that the uncultured microorganisms with these 16S rDNA sequences were sulfate-reducing organisms, the physiology of the strain FW-1a, which does not reduce sulfate, indicates that these organisms are just as likely to be Fe(III) reducers. These results further demonstrate that Fe(III) may be helpful for recovering as-yet-uncultured microorganisms from hydrothermal environments and illustrate that caution ... Article in Journal/Newspaper Iceland ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 68 4 1735 1742 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT It has recently been recognized that the ability to use Fe(III) as a terminal electron acceptor is a highly conserved characteristic in hyperthermophilic microorganisms. This suggests that it may be possible to recover as-yet-uncultured hyperthermophiles in pure culture if Fe(III) is used as an electron acceptor. As part of a study of the microbial diversity of the Obsidian Pool area in Yellowstone National Park, Wyo., hot sediment samples were used as the inoculum for enrichment cultures in media containing hydrogen as the sole electron donor and poorly crystalline Fe(III) oxide as the electron acceptor. A pure culture was recovered on solidified, Fe(III) oxide medium. The isolate, designated FW-1a, is a hyperthermophilic anaerobe that grows exclusively by coupling hydrogen oxidation to the reduction of poorly crystalline Fe(III) oxide. Organic carbon is not required for growth. Magnetite is the end product of Fe(III) oxide reduction under the culture conditions evaluated. The cells are rod shaped, about 0.5 μm by 1.0 to 1.2 μm, and motile and have a single flagellum. Strain FW-1a grows at circumneutral pH, at freshwater salinities, and at temperatures of between 65 and 100°C with an optimum of 85 to 90°C. To our knowledge this is the highest temperature optimum of any organism in the Bacteria . Analysis of the 16S ribosomal DNA (rDNA) sequence of strain FW-1a places it within the Bacteria , most closely related to abundant but uncultured microorganisms whose 16S rDNA sequences have been previously recovered from Obsidian Pool and a terrestrial hot spring in Iceland. While previous studies inferred that the uncultured microorganisms with these 16S rDNA sequences were sulfate-reducing organisms, the physiology of the strain FW-1a, which does not reduce sulfate, indicates that these organisms are just as likely to be Fe(III) reducers. These results further demonstrate that Fe(III) may be helpful for recovering as-yet-uncultured microorganisms from hydrothermal environments and illustrate that caution ... |
format |
Article in Journal/Newspaper |
author |
Kashefi, Kazem Holmes, Dawn E. Reysenbach, Anna-Louise Lovley, Derek R. |
spellingShingle |
Kashefi, Kazem Holmes, Dawn E. Reysenbach, Anna-Louise Lovley, Derek R. Use of Fe(III) as an Electron Acceptor To Recover Previously Uncultured Hyperthermophiles: Isolation and Characterization of Geothermobacterium ferrireducens gen. nov., sp. nov |
author_facet |
Kashefi, Kazem Holmes, Dawn E. Reysenbach, Anna-Louise Lovley, Derek R. |
author_sort |
Kashefi, Kazem |
title |
Use of Fe(III) as an Electron Acceptor To Recover Previously Uncultured Hyperthermophiles: Isolation and Characterization of Geothermobacterium ferrireducens gen. nov., sp. nov |
title_short |
Use of Fe(III) as an Electron Acceptor To Recover Previously Uncultured Hyperthermophiles: Isolation and Characterization of Geothermobacterium ferrireducens gen. nov., sp. nov |
title_full |
Use of Fe(III) as an Electron Acceptor To Recover Previously Uncultured Hyperthermophiles: Isolation and Characterization of Geothermobacterium ferrireducens gen. nov., sp. nov |
title_fullStr |
Use of Fe(III) as an Electron Acceptor To Recover Previously Uncultured Hyperthermophiles: Isolation and Characterization of Geothermobacterium ferrireducens gen. nov., sp. nov |
title_full_unstemmed |
Use of Fe(III) as an Electron Acceptor To Recover Previously Uncultured Hyperthermophiles: Isolation and Characterization of Geothermobacterium ferrireducens gen. nov., sp. nov |
title_sort |
use of fe(iii) as an electron acceptor to recover previously uncultured hyperthermophiles: isolation and characterization of geothermobacterium ferrireducens gen. nov., sp. nov |
publisher |
American Society for Microbiology |
publishDate |
2002 |
url |
http://dx.doi.org/10.1128/aem.68.4.1735-1742.2002 https://journals.asm.org/doi/pdf/10.1128/AEM.68.4.1735-1742.2002 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Applied and Environmental Microbiology volume 68, issue 4, page 1735-1742 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.68.4.1735-1742.2002 |
container_title |
Applied and Environmental Microbiology |
container_volume |
68 |
container_issue |
4 |
container_start_page |
1735 |
op_container_end_page |
1742 |
_version_ |
1811640409687851008 |