Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria
ABSTRACT A PCR approach was used to construct a database of nasA genes (called narB genes in cyanobacteria) and to detect the genetic potential for heterotrophic bacterial nitrate utilization in marine environments. A nasA -specific PCR primer set that could be used to selectively amplify the nasA g...
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Online Access: | http://dx.doi.org/10.1128/aem.67.11.5343-5348.2001 https://journals.asm.org/doi/pdf/10.1128/AEM.67.11.5343-5348.2001 |
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crasmicro:10.1128/aem.67.11.5343-5348.2001 2024-09-15T17:57:52+00:00 Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria Allen, Andrew E. Booth, Melissa G. Frischer, Marc E. Verity, Peter G. Zehr, Jonathan P. Zani, Sabino 2001 http://dx.doi.org/10.1128/aem.67.11.5343-5348.2001 https://journals.asm.org/doi/pdf/10.1128/AEM.67.11.5343-5348.2001 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 67, issue 11, page 5343-5348 ISSN 0099-2240 1098-5336 journal-article 2001 crasmicro https://doi.org/10.1128/aem.67.11.5343-5348.2001 2024-08-12T04:06:13Z ABSTRACT A PCR approach was used to construct a database of nasA genes (called narB genes in cyanobacteria) and to detect the genetic potential for heterotrophic bacterial nitrate utilization in marine environments. A nasA -specific PCR primer set that could be used to selectively amplify the nasA gene from heterotrophic bacteria was designed. Using seawater DNA extracts obtained from microbial communities in the South Atlantic Bight, the Barents Sea, and the North Pacific Gyre, we PCR amplified and sequenced nasA genes. Our results indicate that several groups of heterotrophic bacterial nasA genes are common and widely distributed in oceanic environments. Article in Journal/Newspaper Barents Sea ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 67 11 5343 5348 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT A PCR approach was used to construct a database of nasA genes (called narB genes in cyanobacteria) and to detect the genetic potential for heterotrophic bacterial nitrate utilization in marine environments. A nasA -specific PCR primer set that could be used to selectively amplify the nasA gene from heterotrophic bacteria was designed. Using seawater DNA extracts obtained from microbial communities in the South Atlantic Bight, the Barents Sea, and the North Pacific Gyre, we PCR amplified and sequenced nasA genes. Our results indicate that several groups of heterotrophic bacterial nasA genes are common and widely distributed in oceanic environments. |
format |
Article in Journal/Newspaper |
author |
Allen, Andrew E. Booth, Melissa G. Frischer, Marc E. Verity, Peter G. Zehr, Jonathan P. Zani, Sabino |
spellingShingle |
Allen, Andrew E. Booth, Melissa G. Frischer, Marc E. Verity, Peter G. Zehr, Jonathan P. Zani, Sabino Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria |
author_facet |
Allen, Andrew E. Booth, Melissa G. Frischer, Marc E. Verity, Peter G. Zehr, Jonathan P. Zani, Sabino |
author_sort |
Allen, Andrew E. |
title |
Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria |
title_short |
Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria |
title_full |
Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria |
title_fullStr |
Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria |
title_full_unstemmed |
Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria |
title_sort |
diversity and detection of nitrate assimilation genes in marine bacteria |
publisher |
American Society for Microbiology |
publishDate |
2001 |
url |
http://dx.doi.org/10.1128/aem.67.11.5343-5348.2001 https://journals.asm.org/doi/pdf/10.1128/AEM.67.11.5343-5348.2001 |
genre |
Barents Sea |
genre_facet |
Barents Sea |
op_source |
Applied and Environmental Microbiology volume 67, issue 11, page 5343-5348 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.67.11.5343-5348.2001 |
container_title |
Applied and Environmental Microbiology |
container_volume |
67 |
container_issue |
11 |
container_start_page |
5343 |
op_container_end_page |
5348 |
_version_ |
1810434071987224576 |