Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil
ABSTRACT Degradation of petroleum hydrocarbons was monitored in microcosms with diesel fuel-contaminated Arctic tundra soil incubated for 48 days at low temperatures (−5, 0, and 7°C). An additional treatment was incubation for alternating 24-h periods at 7 and −5°C. Hydrocarbons were biodegraded at...
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American Society for Microbiology
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Online Access: | http://dx.doi.org/10.1128/aem.67.11.5107-5112.2001 https://journals.asm.org/doi/pdf/10.1128/AEM.67.11.5107-5112.2001 |
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crasmicro:10.1128/aem.67.11.5107-5112.2001 2024-09-15T18:39:42+00:00 Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil Eriksson, Mikael Ka, Jong-Ok Mohn, William W. 2001 http://dx.doi.org/10.1128/aem.67.11.5107-5112.2001 https://journals.asm.org/doi/pdf/10.1128/AEM.67.11.5107-5112.2001 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 67, issue 11, page 5107-5112 ISSN 0099-2240 1098-5336 journal-article 2001 crasmicro https://doi.org/10.1128/aem.67.11.5107-5112.2001 2024-08-12T04:06:38Z ABSTRACT Degradation of petroleum hydrocarbons was monitored in microcosms with diesel fuel-contaminated Arctic tundra soil incubated for 48 days at low temperatures (−5, 0, and 7°C). An additional treatment was incubation for alternating 24-h periods at 7 and −5°C. Hydrocarbons were biodegraded at or above 0°C, and freeze-thaw cycles may have actually stimulated hydrocarbon biodegradation. Total petroleum hydrocarbon (TPH) removal over 48 days in the 7, 0, and 7 and −5°C treatments, respectively, was 450, 300, and 600 μg/g of soil. No TPH removal was observed at −5°C. Total carbon dioxide production suggested that TPH removal was due to biological mineralization. Bacterial metabolic activity, indicated by RNA/DNA ratios, was higher in the middle of the experiment (day 21) than at the start, in agreement with measured hydrocarbon removal and carbon dioxide production activities. The total numbers of culturable heterotrophs and of hydrocarbon degraders did not change significantly over the 48 days of incubation in any of the treatments. At the end of the experiment, bacterial community structure, evaluated by ribosomal intergenic spacer length analysis, was very similar in all of the treatments but the alternating 7 and −5°C treatment. Article in Journal/Newspaper Tundra ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 67 11 5107 5112 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
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English |
description |
ABSTRACT Degradation of petroleum hydrocarbons was monitored in microcosms with diesel fuel-contaminated Arctic tundra soil incubated for 48 days at low temperatures (−5, 0, and 7°C). An additional treatment was incubation for alternating 24-h periods at 7 and −5°C. Hydrocarbons were biodegraded at or above 0°C, and freeze-thaw cycles may have actually stimulated hydrocarbon biodegradation. Total petroleum hydrocarbon (TPH) removal over 48 days in the 7, 0, and 7 and −5°C treatments, respectively, was 450, 300, and 600 μg/g of soil. No TPH removal was observed at −5°C. Total carbon dioxide production suggested that TPH removal was due to biological mineralization. Bacterial metabolic activity, indicated by RNA/DNA ratios, was higher in the middle of the experiment (day 21) than at the start, in agreement with measured hydrocarbon removal and carbon dioxide production activities. The total numbers of culturable heterotrophs and of hydrocarbon degraders did not change significantly over the 48 days of incubation in any of the treatments. At the end of the experiment, bacterial community structure, evaluated by ribosomal intergenic spacer length analysis, was very similar in all of the treatments but the alternating 7 and −5°C treatment. |
format |
Article in Journal/Newspaper |
author |
Eriksson, Mikael Ka, Jong-Ok Mohn, William W. |
spellingShingle |
Eriksson, Mikael Ka, Jong-Ok Mohn, William W. Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil |
author_facet |
Eriksson, Mikael Ka, Jong-Ok Mohn, William W. |
author_sort |
Eriksson, Mikael |
title |
Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil |
title_short |
Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil |
title_full |
Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil |
title_fullStr |
Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil |
title_full_unstemmed |
Effects of Low Temperature and Freeze-Thaw Cycles on Hydrocarbon Biodegradation in Arctic Tundra Soil |
title_sort |
effects of low temperature and freeze-thaw cycles on hydrocarbon biodegradation in arctic tundra soil |
publisher |
American Society for Microbiology |
publishDate |
2001 |
url |
http://dx.doi.org/10.1128/aem.67.11.5107-5112.2001 https://journals.asm.org/doi/pdf/10.1128/AEM.67.11.5107-5112.2001 |
genre |
Tundra |
genre_facet |
Tundra |
op_source |
Applied and Environmental Microbiology volume 67, issue 11, page 5107-5112 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.67.11.5107-5112.2001 |
container_title |
Applied and Environmental Microbiology |
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67 |
container_issue |
11 |
container_start_page |
5107 |
op_container_end_page |
5112 |
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1810484045941833728 |