A Few Cosmopolitan Phylotypes Dominate Planktonic Archaeal Assemblages in Widely Different Oceanic Provinces
ABSTRACT We compared the phylogenetic compositions of marine planktonic archaeal populations in different marine provinces. Samples from eight different environments were collected at two depths (surface and aphotic zone), and 16 genetic libraries of PCR-amplified archaeal 16S rRNA genes were constr...
Published in: | Applied and Environmental Microbiology |
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American Society for Microbiology
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Online Access: | http://dx.doi.org/10.1128/aem.66.5.1777-1787.2000 https://journals.asm.org/doi/pdf/10.1128/AEM.66.5.1777-1787.2000 |
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crasmicro:10.1128/aem.66.5.1777-1787.2000 2024-10-06T13:44:08+00:00 A Few Cosmopolitan Phylotypes Dominate Planktonic Archaeal Assemblages in Widely Different Oceanic Provinces Massana, Ramon DeLong, Edward F. Pedrós-Alió, Carlos 2000 http://dx.doi.org/10.1128/aem.66.5.1777-1787.2000 https://journals.asm.org/doi/pdf/10.1128/AEM.66.5.1777-1787.2000 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 66, issue 5, page 1777-1787 ISSN 0099-2240 1098-5336 journal-article 2000 crasmicro https://doi.org/10.1128/aem.66.5.1777-1787.2000 2024-09-09T04:16:22Z ABSTRACT We compared the phylogenetic compositions of marine planktonic archaeal populations in different marine provinces. Samples from eight different environments were collected at two depths (surface and aphotic zone), and 16 genetic libraries of PCR-amplified archaeal 16S rRNA genes were constructed. The libraries were analyzed by using a three-step hierarchical approach. Membrane hybridization experiments revealed that most of the archaeal clones were affiliated with one of the two groups of marine archaea described previously, crenarchaeotal group I and euryarchaeotal group II. One of the 2,328 ribosomal DNA clones analyzed was related to a different euryarchaeal lineage, which was recently recovered from deep-water marine plankton. In temperate regions (Pacific Ocean, Atlantic Ocean, and Mediterranean Sea) both major groups were found at the two depths investigated; group II predominated at the surface, and group I predominated at depth. In Antarctic and subantarctic waters group II was practically absent. The clonal compositions of archaeal libraries were investigated by performing a restriction fragment length polymorphism (RFLP) analysis with two tetrameric restriction enzymes, which defined discrete operational taxonomic units (OTUs). The OTUs defined in this way were phylogenetically consistent; clones belonging to the same OTU were closely related. The clonal diversity as determined by the RFLP analysis was low, and most libraries were dominated by only one or two OTUs. Some OTUs were found in samples obtained from very distant places, indicating that some phylotypes were ubiquitous. A tree containing one example of each OTU detected was constructed, and this tree revealed that there were several clusters within archaeal group I and group II. The members of some of these clusters had different depth distributions. Article in Journal/Newspaper Antarc* Antarctic ASM Journals (American Society for Microbiology) Antarctic Pacific Applied and Environmental Microbiology 66 5 1777 1787 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT We compared the phylogenetic compositions of marine planktonic archaeal populations in different marine provinces. Samples from eight different environments were collected at two depths (surface and aphotic zone), and 16 genetic libraries of PCR-amplified archaeal 16S rRNA genes were constructed. The libraries were analyzed by using a three-step hierarchical approach. Membrane hybridization experiments revealed that most of the archaeal clones were affiliated with one of the two groups of marine archaea described previously, crenarchaeotal group I and euryarchaeotal group II. One of the 2,328 ribosomal DNA clones analyzed was related to a different euryarchaeal lineage, which was recently recovered from deep-water marine plankton. In temperate regions (Pacific Ocean, Atlantic Ocean, and Mediterranean Sea) both major groups were found at the two depths investigated; group II predominated at the surface, and group I predominated at depth. In Antarctic and subantarctic waters group II was practically absent. The clonal compositions of archaeal libraries were investigated by performing a restriction fragment length polymorphism (RFLP) analysis with two tetrameric restriction enzymes, which defined discrete operational taxonomic units (OTUs). The OTUs defined in this way were phylogenetically consistent; clones belonging to the same OTU were closely related. The clonal diversity as determined by the RFLP analysis was low, and most libraries were dominated by only one or two OTUs. Some OTUs were found in samples obtained from very distant places, indicating that some phylotypes were ubiquitous. A tree containing one example of each OTU detected was constructed, and this tree revealed that there were several clusters within archaeal group I and group II. The members of some of these clusters had different depth distributions. |
format |
Article in Journal/Newspaper |
author |
Massana, Ramon DeLong, Edward F. Pedrós-Alió, Carlos |
spellingShingle |
Massana, Ramon DeLong, Edward F. Pedrós-Alió, Carlos A Few Cosmopolitan Phylotypes Dominate Planktonic Archaeal Assemblages in Widely Different Oceanic Provinces |
author_facet |
Massana, Ramon DeLong, Edward F. Pedrós-Alió, Carlos |
author_sort |
Massana, Ramon |
title |
A Few Cosmopolitan Phylotypes Dominate Planktonic Archaeal Assemblages in Widely Different Oceanic Provinces |
title_short |
A Few Cosmopolitan Phylotypes Dominate Planktonic Archaeal Assemblages in Widely Different Oceanic Provinces |
title_full |
A Few Cosmopolitan Phylotypes Dominate Planktonic Archaeal Assemblages in Widely Different Oceanic Provinces |
title_fullStr |
A Few Cosmopolitan Phylotypes Dominate Planktonic Archaeal Assemblages in Widely Different Oceanic Provinces |
title_full_unstemmed |
A Few Cosmopolitan Phylotypes Dominate Planktonic Archaeal Assemblages in Widely Different Oceanic Provinces |
title_sort |
few cosmopolitan phylotypes dominate planktonic archaeal assemblages in widely different oceanic provinces |
publisher |
American Society for Microbiology |
publishDate |
2000 |
url |
http://dx.doi.org/10.1128/aem.66.5.1777-1787.2000 https://journals.asm.org/doi/pdf/10.1128/AEM.66.5.1777-1787.2000 |
geographic |
Antarctic Pacific |
geographic_facet |
Antarctic Pacific |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Applied and Environmental Microbiology volume 66, issue 5, page 1777-1787 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.66.5.1777-1787.2000 |
container_title |
Applied and Environmental Microbiology |
container_volume |
66 |
container_issue |
5 |
container_start_page |
1777 |
op_container_end_page |
1787 |
_version_ |
1812182538872946688 |