Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments
ABSTRACT Thirteen psychrophilic sulfate-reducing isolates from two permanently cold fjords of the Arctic island Spitsbergen (Hornsund and Storfjord) were phylogenetically analyzed. They all belonged to the δ subclass of Proteobacteria and were widely distributed within this group, indicating that ps...
Published in: | Applied and Environmental Microbiology |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
American Society for Microbiology
1999
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1128/aem.65.9.3976-3981.1999 https://journals.asm.org/doi/pdf/10.1128/AEM.65.9.3976-3981.1999 |
id |
crasmicro:10.1128/aem.65.9.3976-3981.1999 |
---|---|
record_format |
openpolar |
spelling |
crasmicro:10.1128/aem.65.9.3976-3981.1999 2024-03-03T08:41:40+00:00 Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments Sahm, Kerstin Knoblauch, Christian Amann, Rudolf 1999 http://dx.doi.org/10.1128/aem.65.9.3976-3981.1999 https://journals.asm.org/doi/pdf/10.1128/AEM.65.9.3976-3981.1999 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 65, issue 9, page 3976-3981 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 1999 crasmicro https://doi.org/10.1128/aem.65.9.3976-3981.1999 2024-02-03T23:13:58Z ABSTRACT Thirteen psychrophilic sulfate-reducing isolates from two permanently cold fjords of the Arctic island Spitsbergen (Hornsund and Storfjord) were phylogenetically analyzed. They all belonged to the δ subclass of Proteobacteria and were widely distributed within this group, indicating that psychrophily is a polyphyletic property. A new 16S rRNA-directed oligonucleotide probe was designed against the largest coherent cluster of these isolates. The new probe, as well as a set of available probes, was applied in rRNA slot blot hybridization to investigate the composition of the sulfate-reducing bacterial community in the sediments. rRNA related to the new cluster of incompletely oxidizing, psychrophilic isolates made up 1.4 to 20.9% of eubacterial rRNA at Storfjord and 0.6 to 3.5% of eubacterial rRNA at Hornsund. This group was the second-most-abundant group of sulfate reducers at these sites. Denaturing gradient gel electrophoresis and hybridization analysis showed bands identical to those produced by our isolates. The data indicate that the psychrophilic isolates are quantitatively important in Svalbard sediments. Article in Journal/Newspaper Arctic Hornsund Svalbard Spitsbergen ASM Journals (American Society for Microbiology) Arctic Svalbard Hornsund ENVELOPE(15.865,15.865,76.979,76.979) Arctic Island ENVELOPE(-74.766,-74.766,62.234,62.234) Applied and Environmental Microbiology 65 9 3976 3981 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Sahm, Kerstin Knoblauch, Christian Amann, Rudolf Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
ABSTRACT Thirteen psychrophilic sulfate-reducing isolates from two permanently cold fjords of the Arctic island Spitsbergen (Hornsund and Storfjord) were phylogenetically analyzed. They all belonged to the δ subclass of Proteobacteria and were widely distributed within this group, indicating that psychrophily is a polyphyletic property. A new 16S rRNA-directed oligonucleotide probe was designed against the largest coherent cluster of these isolates. The new probe, as well as a set of available probes, was applied in rRNA slot blot hybridization to investigate the composition of the sulfate-reducing bacterial community in the sediments. rRNA related to the new cluster of incompletely oxidizing, psychrophilic isolates made up 1.4 to 20.9% of eubacterial rRNA at Storfjord and 0.6 to 3.5% of eubacterial rRNA at Hornsund. This group was the second-most-abundant group of sulfate reducers at these sites. Denaturing gradient gel electrophoresis and hybridization analysis showed bands identical to those produced by our isolates. The data indicate that the psychrophilic isolates are quantitatively important in Svalbard sediments. |
format |
Article in Journal/Newspaper |
author |
Sahm, Kerstin Knoblauch, Christian Amann, Rudolf |
author_facet |
Sahm, Kerstin Knoblauch, Christian Amann, Rudolf |
author_sort |
Sahm, Kerstin |
title |
Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments |
title_short |
Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments |
title_full |
Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments |
title_fullStr |
Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments |
title_full_unstemmed |
Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments |
title_sort |
phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine arctic sediments |
publisher |
American Society for Microbiology |
publishDate |
1999 |
url |
http://dx.doi.org/10.1128/aem.65.9.3976-3981.1999 https://journals.asm.org/doi/pdf/10.1128/AEM.65.9.3976-3981.1999 |
long_lat |
ENVELOPE(15.865,15.865,76.979,76.979) ENVELOPE(-74.766,-74.766,62.234,62.234) |
geographic |
Arctic Svalbard Hornsund Arctic Island |
geographic_facet |
Arctic Svalbard Hornsund Arctic Island |
genre |
Arctic Hornsund Svalbard Spitsbergen |
genre_facet |
Arctic Hornsund Svalbard Spitsbergen |
op_source |
Applied and Environmental Microbiology volume 65, issue 9, page 3976-3981 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.65.9.3976-3981.1999 |
container_title |
Applied and Environmental Microbiology |
container_volume |
65 |
container_issue |
9 |
container_start_page |
3976 |
op_container_end_page |
3981 |
_version_ |
1792497317255839744 |