Diel Rhythms in Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase and Glutamine Synthetase Gene Expression in a Natural Population of Marine Picoplanktonic Cyanobacteria ( Synechococcus spp.)
ABSTRACT Diel periodicity in the expression of key genes involved in carbon and nitrogen assimilation in marine Synechococcus spp. was investigated in a natural population growing in the surface waters of a cyclonic eddy in the northeast Atlantic Ocean. Synechococcus sp. cell concentrations within t...
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Online Access: | http://dx.doi.org/10.1128/aem.65.8.3651-3659.1999 https://journals.asm.org/doi/pdf/10.1128/AEM.65.8.3651-3659.1999 |
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crasmicro:10.1128/aem.65.8.3651-3659.1999 2023-11-05T03:44:17+01:00 Diel Rhythms in Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase and Glutamine Synthetase Gene Expression in a Natural Population of Marine Picoplanktonic Cyanobacteria ( Synechococcus spp.) Wyman, Michael 1999 http://dx.doi.org/10.1128/aem.65.8.3651-3659.1999 https://journals.asm.org/doi/pdf/10.1128/AEM.65.8.3651-3659.1999 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 65, issue 8, page 3651-3659 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 1999 crasmicro https://doi.org/10.1128/aem.65.8.3651-3659.1999 2023-10-09T16:00:37Z ABSTRACT Diel periodicity in the expression of key genes involved in carbon and nitrogen assimilation in marine Synechococcus spp. was investigated in a natural population growing in the surface waters of a cyclonic eddy in the northeast Atlantic Ocean. Synechococcus sp. cell concentrations within the upper mixed layer showed a net increase of three- to fourfold during the course of the experiment (13 to 22 July 1991), the population undergoing approximately one synchronous division per day. Consistent with the observed temporal pattern of phycoerythrin (CpeBA) biosynthesis, comparatively little variation was found in cpeBA mRNA abundance during either of the diel cycles investigated. In marked contrast, the relative abundance of transcripts originating from the genes encoding the large subunit of ribulose bisphosphate carboxylase/oxygenase ( rbcL ) and glutamine synthetase ( glnA ) showed considerable systematic temporal variation and oscillated during the course of each diel cycle in a reciprocal rhythm. Whereas activation of rbcL transcription was clearly not light dependent, expression of glnA appeared sensitive to endogenous changes in the physiological demands for nitrogen that arise as a natural consequence of temporal periodicity in photosynthetic carbon assimilation. The data presented support the hypothesis that a degree of temporal separation may exist between the most active periods of carbon and nitrogen assimilation in natural populations of marine Synecoccoccus spp. Article in Journal/Newspaper Northeast Atlantic ASM Journals (American Society for Microbiology - via Crossref) Applied and Environmental Microbiology 65 8 3651 3659 |
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Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Wyman, Michael Diel Rhythms in Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase and Glutamine Synthetase Gene Expression in a Natural Population of Marine Picoplanktonic Cyanobacteria ( Synechococcus spp.) |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
ABSTRACT Diel periodicity in the expression of key genes involved in carbon and nitrogen assimilation in marine Synechococcus spp. was investigated in a natural population growing in the surface waters of a cyclonic eddy in the northeast Atlantic Ocean. Synechococcus sp. cell concentrations within the upper mixed layer showed a net increase of three- to fourfold during the course of the experiment (13 to 22 July 1991), the population undergoing approximately one synchronous division per day. Consistent with the observed temporal pattern of phycoerythrin (CpeBA) biosynthesis, comparatively little variation was found in cpeBA mRNA abundance during either of the diel cycles investigated. In marked contrast, the relative abundance of transcripts originating from the genes encoding the large subunit of ribulose bisphosphate carboxylase/oxygenase ( rbcL ) and glutamine synthetase ( glnA ) showed considerable systematic temporal variation and oscillated during the course of each diel cycle in a reciprocal rhythm. Whereas activation of rbcL transcription was clearly not light dependent, expression of glnA appeared sensitive to endogenous changes in the physiological demands for nitrogen that arise as a natural consequence of temporal periodicity in photosynthetic carbon assimilation. The data presented support the hypothesis that a degree of temporal separation may exist between the most active periods of carbon and nitrogen assimilation in natural populations of marine Synecoccoccus spp. |
format |
Article in Journal/Newspaper |
author |
Wyman, Michael |
author_facet |
Wyman, Michael |
author_sort |
Wyman, Michael |
title |
Diel Rhythms in Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase and Glutamine Synthetase Gene Expression in a Natural Population of Marine Picoplanktonic Cyanobacteria ( Synechococcus spp.) |
title_short |
Diel Rhythms in Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase and Glutamine Synthetase Gene Expression in a Natural Population of Marine Picoplanktonic Cyanobacteria ( Synechococcus spp.) |
title_full |
Diel Rhythms in Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase and Glutamine Synthetase Gene Expression in a Natural Population of Marine Picoplanktonic Cyanobacteria ( Synechococcus spp.) |
title_fullStr |
Diel Rhythms in Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase and Glutamine Synthetase Gene Expression in a Natural Population of Marine Picoplanktonic Cyanobacteria ( Synechococcus spp.) |
title_full_unstemmed |
Diel Rhythms in Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase and Glutamine Synthetase Gene Expression in a Natural Population of Marine Picoplanktonic Cyanobacteria ( Synechococcus spp.) |
title_sort |
diel rhythms in ribulose-1,5-bisphosphate carboxylase/oxygenase and glutamine synthetase gene expression in a natural population of marine picoplanktonic cyanobacteria ( synechococcus spp.) |
publisher |
American Society for Microbiology |
publishDate |
1999 |
url |
http://dx.doi.org/10.1128/aem.65.8.3651-3659.1999 https://journals.asm.org/doi/pdf/10.1128/AEM.65.8.3651-3659.1999 |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_source |
Applied and Environmental Microbiology volume 65, issue 8, page 3651-3659 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.65.8.3651-3659.1999 |
container_title |
Applied and Environmental Microbiology |
container_volume |
65 |
container_issue |
8 |
container_start_page |
3651 |
op_container_end_page |
3659 |
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1781703769053986816 |