Identification of a Marine Agarolytic Pseudoalteromonas Isolate and Characterization of Its Extracellular Agarase
ABSTRACT The phenotypic and agarolytic features of an unidentified marine bacteria that was isolated from the southern Pacific coast was investigated. The strain was gram negative, obligately aerobic, and polarly flagellated. On the basis of several phenotypic characters and a phylogenetic analysis...
Published in: | Applied and Environmental Microbiology |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
American Society for Microbiology
1998
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1128/aem.64.11.4378-4383.1998 https://journals.asm.org/doi/pdf/10.1128/AEM.64.11.4378-4383.1998 |
id |
crasmicro:10.1128/aem.64.11.4378-4383.1998 |
---|---|
record_format |
openpolar |
spelling |
crasmicro:10.1128/aem.64.11.4378-4383.1998 2024-09-15T17:45:34+00:00 Identification of a Marine Agarolytic Pseudoalteromonas Isolate and Characterization of Its Extracellular Agarase Vera, Jorge Alvarez, Raul Murano, Erminio Slebe, Juan Carlos Leon, Oscar 1998 http://dx.doi.org/10.1128/aem.64.11.4378-4383.1998 https://journals.asm.org/doi/pdf/10.1128/AEM.64.11.4378-4383.1998 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 64, issue 11, page 4378-4383 ISSN 0099-2240 1098-5336 journal-article 1998 crasmicro https://doi.org/10.1128/aem.64.11.4378-4383.1998 2024-07-29T04:09:02Z ABSTRACT The phenotypic and agarolytic features of an unidentified marine bacteria that was isolated from the southern Pacific coast was investigated. The strain was gram negative, obligately aerobic, and polarly flagellated. On the basis of several phenotypic characters and a phylogenetic analysis of the genes coding for the 16S rRNA, this strain was identified as Pseudoalteromonas antarctica strain N-1. In solid agar, this isolate produced a diffusible agarase that caused agar softening around the colonies. An extracellular agarase was purified by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography on DEAE-cellulose. The purified protein was determined to be homogeneous on the basis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it had a molecular mass of 33 kDa. The enzyme hydrolyzed the β-1,4-glycosydic linkages of agar, yielding neoagarotetraose and neoagarohexaose as the main products, and exhibited maximal activity at pH 7. The enzyme was stable at temperatures up to 30°C, and its activity was not affected by salt concentrations up to 0.5 M NaCl. Article in Journal/Newspaper Antarc* Antarctica ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 64 11 4378 4383 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
description |
ABSTRACT The phenotypic and agarolytic features of an unidentified marine bacteria that was isolated from the southern Pacific coast was investigated. The strain was gram negative, obligately aerobic, and polarly flagellated. On the basis of several phenotypic characters and a phylogenetic analysis of the genes coding for the 16S rRNA, this strain was identified as Pseudoalteromonas antarctica strain N-1. In solid agar, this isolate produced a diffusible agarase that caused agar softening around the colonies. An extracellular agarase was purified by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography on DEAE-cellulose. The purified protein was determined to be homogeneous on the basis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it had a molecular mass of 33 kDa. The enzyme hydrolyzed the β-1,4-glycosydic linkages of agar, yielding neoagarotetraose and neoagarohexaose as the main products, and exhibited maximal activity at pH 7. The enzyme was stable at temperatures up to 30°C, and its activity was not affected by salt concentrations up to 0.5 M NaCl. |
format |
Article in Journal/Newspaper |
author |
Vera, Jorge Alvarez, Raul Murano, Erminio Slebe, Juan Carlos Leon, Oscar |
spellingShingle |
Vera, Jorge Alvarez, Raul Murano, Erminio Slebe, Juan Carlos Leon, Oscar Identification of a Marine Agarolytic Pseudoalteromonas Isolate and Characterization of Its Extracellular Agarase |
author_facet |
Vera, Jorge Alvarez, Raul Murano, Erminio Slebe, Juan Carlos Leon, Oscar |
author_sort |
Vera, Jorge |
title |
Identification of a Marine Agarolytic Pseudoalteromonas Isolate and Characterization of Its Extracellular Agarase |
title_short |
Identification of a Marine Agarolytic Pseudoalteromonas Isolate and Characterization of Its Extracellular Agarase |
title_full |
Identification of a Marine Agarolytic Pseudoalteromonas Isolate and Characterization of Its Extracellular Agarase |
title_fullStr |
Identification of a Marine Agarolytic Pseudoalteromonas Isolate and Characterization of Its Extracellular Agarase |
title_full_unstemmed |
Identification of a Marine Agarolytic Pseudoalteromonas Isolate and Characterization of Its Extracellular Agarase |
title_sort |
identification of a marine agarolytic pseudoalteromonas isolate and characterization of its extracellular agarase |
publisher |
American Society for Microbiology |
publishDate |
1998 |
url |
http://dx.doi.org/10.1128/aem.64.11.4378-4383.1998 https://journals.asm.org/doi/pdf/10.1128/AEM.64.11.4378-4383.1998 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Applied and Environmental Microbiology volume 64, issue 11, page 4378-4383 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.64.11.4378-4383.1998 |
container_title |
Applied and Environmental Microbiology |
container_volume |
64 |
container_issue |
11 |
container_start_page |
4378 |
op_container_end_page |
4383 |
_version_ |
1810493443393191936 |