Genomic variation of aquatic birnaviruses analyzed with restriction fragment length polymorphisms
Aquatic birnaviruses are the most ubiquitous and diverse group of viruses in the family Birnaviridae. Several cause different diseases in a variety of fish species, such as infectious pancreatic necrosis virus in salmonids in North America, Europe, and Asia and European eel virus in eel in Asia. Mos...
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Online Access: | http://dx.doi.org/10.1128/aem.62.7.2513-2520.1996 https://journals.asm.org/doi/pdf/10.1128/aem.62.7.2513-2520.1996 |
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crasmicro:10.1128/aem.62.7.2513-2520.1996 2023-11-05T03:41:49+01:00 Genomic variation of aquatic birnaviruses analyzed with restriction fragment length polymorphisms Lee, M K Blake, S L Singer, J T Nicholson, B L 1996 http://dx.doi.org/10.1128/aem.62.7.2513-2520.1996 https://journals.asm.org/doi/pdf/10.1128/aem.62.7.2513-2520.1996 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 62, issue 7, page 2513-2520 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 1996 crasmicro https://doi.org/10.1128/aem.62.7.2513-2520.1996 2023-10-09T16:09:36Z Aquatic birnaviruses are the most ubiquitous and diverse group of viruses in the family Birnaviridae. Several cause different diseases in a variety of fish species, such as infectious pancreatic necrosis virus in salmonids in North America, Europe, and Asia and European eel virus in eel in Asia. Most isolates are antigenically related and belong to a single serogroup (serogroup A) comprising nine serotypes. Previous studies with monoclonal antibodies have demonstrated considerable variation in epitope profiles even among strains within a single serotype. The few studies of genomic variation among these viruses, which have focused on the NS/VP3 coding region, demonstrated the existence of several genogroups that generally did not correlate with antigenic groups. In this study, PCR was used to amplify a 1,180-bp cDNA genomic fragment representing most of the VP2 (the major outer capsid protein) coding region from five serotype A type strains and 17 Asian isolates. The PCR products were digested with nine different restriction enzymes. Restriction fragment length polymorphism profiles demonstrated heterogeneity among the tested viruses; however, the isolates from Asia were closely related to each other. Cluster analysis of the restriction fragment length polymorphism patterns demonstrated that these viruses could be divided into four major genogroups. In contrast to previous studies of variation in the NS/VP3 coding region, these genogroups based on variation in the VP2 coding region correlated with a serological classification based on VP2-specific monoclonal antibody reaction patterns. Furthermore, all Asian isolates tested belonged to one genogroup typified by the serotype type strain Ab. Article in Journal/Newspaper European eel ASM Journals (American Society for Microbiology - via Crossref) Applied and Environmental Microbiology 62 7 2513 2520 |
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Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Lee, M K Blake, S L Singer, J T Nicholson, B L Genomic variation of aquatic birnaviruses analyzed with restriction fragment length polymorphisms |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
Aquatic birnaviruses are the most ubiquitous and diverse group of viruses in the family Birnaviridae. Several cause different diseases in a variety of fish species, such as infectious pancreatic necrosis virus in salmonids in North America, Europe, and Asia and European eel virus in eel in Asia. Most isolates are antigenically related and belong to a single serogroup (serogroup A) comprising nine serotypes. Previous studies with monoclonal antibodies have demonstrated considerable variation in epitope profiles even among strains within a single serotype. The few studies of genomic variation among these viruses, which have focused on the NS/VP3 coding region, demonstrated the existence of several genogroups that generally did not correlate with antigenic groups. In this study, PCR was used to amplify a 1,180-bp cDNA genomic fragment representing most of the VP2 (the major outer capsid protein) coding region from five serotype A type strains and 17 Asian isolates. The PCR products were digested with nine different restriction enzymes. Restriction fragment length polymorphism profiles demonstrated heterogeneity among the tested viruses; however, the isolates from Asia were closely related to each other. Cluster analysis of the restriction fragment length polymorphism patterns demonstrated that these viruses could be divided into four major genogroups. In contrast to previous studies of variation in the NS/VP3 coding region, these genogroups based on variation in the VP2 coding region correlated with a serological classification based on VP2-specific monoclonal antibody reaction patterns. Furthermore, all Asian isolates tested belonged to one genogroup typified by the serotype type strain Ab. |
format |
Article in Journal/Newspaper |
author |
Lee, M K Blake, S L Singer, J T Nicholson, B L |
author_facet |
Lee, M K Blake, S L Singer, J T Nicholson, B L |
author_sort |
Lee, M K |
title |
Genomic variation of aquatic birnaviruses analyzed with restriction fragment length polymorphisms |
title_short |
Genomic variation of aquatic birnaviruses analyzed with restriction fragment length polymorphisms |
title_full |
Genomic variation of aquatic birnaviruses analyzed with restriction fragment length polymorphisms |
title_fullStr |
Genomic variation of aquatic birnaviruses analyzed with restriction fragment length polymorphisms |
title_full_unstemmed |
Genomic variation of aquatic birnaviruses analyzed with restriction fragment length polymorphisms |
title_sort |
genomic variation of aquatic birnaviruses analyzed with restriction fragment length polymorphisms |
publisher |
American Society for Microbiology |
publishDate |
1996 |
url |
http://dx.doi.org/10.1128/aem.62.7.2513-2520.1996 https://journals.asm.org/doi/pdf/10.1128/aem.62.7.2513-2520.1996 |
genre |
European eel |
genre_facet |
European eel |
op_source |
Applied and Environmental Microbiology volume 62, issue 7, page 2513-2520 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.62.7.2513-2520.1996 |
container_title |
Applied and Environmental Microbiology |
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62 |
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7 |
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2513 |
op_container_end_page |
2520 |
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1781698471000014848 |