Use of radiolabelled thymidine and leucine to estimate bacterial production in soils from continental antarctica
Tritiated thymidine incorporation (TTI) into DNA was used to examine bacterial production in two soil types from the Robertskollen group of nunataks in northwestern Dronning Maud Land, providing the first estimates of bacterial production in soil habitats on the Antarctic continent. Although estimat...
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American Society for Microbiology
1996
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Online Access: | http://dx.doi.org/10.1128/aem.62.2.694-701.1996 https://journals.asm.org/doi/pdf/10.1128/aem.62.2.694-701.1996 |
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crasmicro:10.1128/aem.62.2.694-701.1996 2023-11-05T03:36:25+01:00 Use of radiolabelled thymidine and leucine to estimate bacterial production in soils from continental antarctica Tibbles, B J Harris, J M 1996 http://dx.doi.org/10.1128/aem.62.2.694-701.1996 https://journals.asm.org/doi/pdf/10.1128/aem.62.2.694-701.1996 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 62, issue 2, page 694-701 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 1996 crasmicro https://doi.org/10.1128/aem.62.2.694-701.1996 2023-10-09T16:14:13Z Tritiated thymidine incorporation (TTI) into DNA was used to examine bacterial production in two soil types from the Robertskollen group of nunataks in northwestern Dronning Maud Land, providing the first estimates of bacterial production in soil habitats on the Antarctic continent. Although estimates of bacterial productivity in soils near to bird nests (344 (plusmn) 422 ng of C g [dry weight](sup-1) h(sup-1)) were higher than those for soils from beneath mosses (175 (plusmn) 90 ng of C g [dry weight](sup-1) h(sup-1); measured by TTI at 10(deg)C), these differences were not significant because of patchiness of bacterial activity (P > 0.05). TTI- and [(sup14)C]leucine ([(sup14)C]Leu)-derived estimates of bacterial production were similar when incubations of 3 h were used, although incubations as short as 1 h were sufficient for measurable uptake of radiolabel. Dual-label incorporation of [(sup3)H]thymidine ([(sup3)H]TdR) into DNA and [(sup14)C]Leu into protein indicated that TTI did not reflect bacterial production of in situ assemblages when incubations were longer than 3 h. Isotope dilution analysis indicated that dilution of the specific activity of exogenously supplied [(sup3)H]TdR by de novo synthesis of TdR precursor could be limited by additions of [(sup3)H]TdR at a concentration of 1 nmol per ca. 115 mg of soil. TTI exhibited a psychrotrophic response to variation in temperature, with a temperature optimum of ca. 15(deg)C and a Q(inf10) value for 0 to 10(deg)C of 2.41. Article in Journal/Newspaper Antarc* Antarctic Antarctica Dronning Maud Land ASM Journals (American Society for Microbiology - via Crossref) Applied and Environmental Microbiology 62 2 694 701 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Tibbles, B J Harris, J M Use of radiolabelled thymidine and leucine to estimate bacterial production in soils from continental antarctica |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
Tritiated thymidine incorporation (TTI) into DNA was used to examine bacterial production in two soil types from the Robertskollen group of nunataks in northwestern Dronning Maud Land, providing the first estimates of bacterial production in soil habitats on the Antarctic continent. Although estimates of bacterial productivity in soils near to bird nests (344 (plusmn) 422 ng of C g [dry weight](sup-1) h(sup-1)) were higher than those for soils from beneath mosses (175 (plusmn) 90 ng of C g [dry weight](sup-1) h(sup-1); measured by TTI at 10(deg)C), these differences were not significant because of patchiness of bacterial activity (P > 0.05). TTI- and [(sup14)C]leucine ([(sup14)C]Leu)-derived estimates of bacterial production were similar when incubations of 3 h were used, although incubations as short as 1 h were sufficient for measurable uptake of radiolabel. Dual-label incorporation of [(sup3)H]thymidine ([(sup3)H]TdR) into DNA and [(sup14)C]Leu into protein indicated that TTI did not reflect bacterial production of in situ assemblages when incubations were longer than 3 h. Isotope dilution analysis indicated that dilution of the specific activity of exogenously supplied [(sup3)H]TdR by de novo synthesis of TdR precursor could be limited by additions of [(sup3)H]TdR at a concentration of 1 nmol per ca. 115 mg of soil. TTI exhibited a psychrotrophic response to variation in temperature, with a temperature optimum of ca. 15(deg)C and a Q(inf10) value for 0 to 10(deg)C of 2.41. |
format |
Article in Journal/Newspaper |
author |
Tibbles, B J Harris, J M |
author_facet |
Tibbles, B J Harris, J M |
author_sort |
Tibbles, B J |
title |
Use of radiolabelled thymidine and leucine to estimate bacterial production in soils from continental antarctica |
title_short |
Use of radiolabelled thymidine and leucine to estimate bacterial production in soils from continental antarctica |
title_full |
Use of radiolabelled thymidine and leucine to estimate bacterial production in soils from continental antarctica |
title_fullStr |
Use of radiolabelled thymidine and leucine to estimate bacterial production in soils from continental antarctica |
title_full_unstemmed |
Use of radiolabelled thymidine and leucine to estimate bacterial production in soils from continental antarctica |
title_sort |
use of radiolabelled thymidine and leucine to estimate bacterial production in soils from continental antarctica |
publisher |
American Society for Microbiology |
publishDate |
1996 |
url |
http://dx.doi.org/10.1128/aem.62.2.694-701.1996 https://journals.asm.org/doi/pdf/10.1128/aem.62.2.694-701.1996 |
genre |
Antarc* Antarctic Antarctica Dronning Maud Land |
genre_facet |
Antarc* Antarctic Antarctica Dronning Maud Land |
op_source |
Applied and Environmental Microbiology volume 62, issue 2, page 694-701 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.62.2.694-701.1996 |
container_title |
Applied and Environmental Microbiology |
container_volume |
62 |
container_issue |
2 |
container_start_page |
694 |
op_container_end_page |
701 |
_version_ |
1781691300256415744 |