Detection of ammonium-oxidizing bacteria of the beta-subclass of the class Proteobacteria in aquatic samples with the PCR
The PCR was used as the basis for the development of a sensitive and specific assay for the detection of ammonium-oxidizing bacteria belonging to the beta-subclass of the class Proteobacteria. PCR primers were selected on the basis of nucleic acid sequence data available for seven species of nitrifi...
Published in: | Applied and Environmental Microbiology |
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American Society for Microbiology
1995
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Online Access: | http://dx.doi.org/10.1128/aem.61.4.1444-1450.1995 https://journals.asm.org/doi/pdf/10.1128/aem.61.4.1444-1450.1995 |
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crasmicro:10.1128/aem.61.4.1444-1450.1995 2023-11-05T03:36:58+01:00 Detection of ammonium-oxidizing bacteria of the beta-subclass of the class Proteobacteria in aquatic samples with the PCR Voytek, M A Ward, B B 1995 http://dx.doi.org/10.1128/aem.61.4.1444-1450.1995 https://journals.asm.org/doi/pdf/10.1128/aem.61.4.1444-1450.1995 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 61, issue 4, page 1444-1450 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 1995 crasmicro https://doi.org/10.1128/aem.61.4.1444-1450.1995 2023-10-09T16:05:32Z The PCR was used as the basis for the development of a sensitive and specific assay for the detection of ammonium-oxidizing bacteria belonging to the beta-subclass of the class Proteobacteria. PCR primers were selected on the basis of nucleic acid sequence data available for seven species of nitrifiers in this subclass. The specificity of the ammonium oxidizer primers was evaluated by testing known strains of nitrifiers, several serotyped environmental nitrifier isolates, and other members of the Proteobacteria, including four very closely related, nonnitrifying species (as determined by rRNA sequence analysis). DNA extracts from 19 bacterio-plankton samples collected from Lake Bonney, Antarctica, and the Southern California Bight were assayed for the presence of ammonium oxidizers. By using a two-stage amplification procedure, ammonium oxidizers were detected in samples collected from both sites. Chemical data collected simultaneously support the occurrence of nitrification and the presence of nitrifiers. This is the first report describing PCR primers specific for ammonium-oxidizing bacteria and the successful amplification of nitrifier genes coding for rRNA from DNA extracts from natural samples. This application of PCR is of particular importance for the detection and study of microbes, such as autotrophic nitrifiers, which are difficult or impossible to isolate from indigenous microbial communities. Article in Journal/Newspaper Antarc* Antarctica ASM Journals (American Society for Microbiology - via Crossref) Applied and Environmental Microbiology 61 4 1444 1450 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Voytek, M A Ward, B B Detection of ammonium-oxidizing bacteria of the beta-subclass of the class Proteobacteria in aquatic samples with the PCR |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
The PCR was used as the basis for the development of a sensitive and specific assay for the detection of ammonium-oxidizing bacteria belonging to the beta-subclass of the class Proteobacteria. PCR primers were selected on the basis of nucleic acid sequence data available for seven species of nitrifiers in this subclass. The specificity of the ammonium oxidizer primers was evaluated by testing known strains of nitrifiers, several serotyped environmental nitrifier isolates, and other members of the Proteobacteria, including four very closely related, nonnitrifying species (as determined by rRNA sequence analysis). DNA extracts from 19 bacterio-plankton samples collected from Lake Bonney, Antarctica, and the Southern California Bight were assayed for the presence of ammonium oxidizers. By using a two-stage amplification procedure, ammonium oxidizers were detected in samples collected from both sites. Chemical data collected simultaneously support the occurrence of nitrification and the presence of nitrifiers. This is the first report describing PCR primers specific for ammonium-oxidizing bacteria and the successful amplification of nitrifier genes coding for rRNA from DNA extracts from natural samples. This application of PCR is of particular importance for the detection and study of microbes, such as autotrophic nitrifiers, which are difficult or impossible to isolate from indigenous microbial communities. |
format |
Article in Journal/Newspaper |
author |
Voytek, M A Ward, B B |
author_facet |
Voytek, M A Ward, B B |
author_sort |
Voytek, M A |
title |
Detection of ammonium-oxidizing bacteria of the beta-subclass of the class Proteobacteria in aquatic samples with the PCR |
title_short |
Detection of ammonium-oxidizing bacteria of the beta-subclass of the class Proteobacteria in aquatic samples with the PCR |
title_full |
Detection of ammonium-oxidizing bacteria of the beta-subclass of the class Proteobacteria in aquatic samples with the PCR |
title_fullStr |
Detection of ammonium-oxidizing bacteria of the beta-subclass of the class Proteobacteria in aquatic samples with the PCR |
title_full_unstemmed |
Detection of ammonium-oxidizing bacteria of the beta-subclass of the class Proteobacteria in aquatic samples with the PCR |
title_sort |
detection of ammonium-oxidizing bacteria of the beta-subclass of the class proteobacteria in aquatic samples with the pcr |
publisher |
American Society for Microbiology |
publishDate |
1995 |
url |
http://dx.doi.org/10.1128/aem.61.4.1444-1450.1995 https://journals.asm.org/doi/pdf/10.1128/aem.61.4.1444-1450.1995 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Applied and Environmental Microbiology volume 61, issue 4, page 1444-1450 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.61.4.1444-1450.1995 |
container_title |
Applied and Environmental Microbiology |
container_volume |
61 |
container_issue |
4 |
container_start_page |
1444 |
op_container_end_page |
1450 |
_version_ |
1781692315650228224 |