Extracellular protease from the antarctic yeast Candida humicola
The psychrotrophic, dimorphic yeast Candida humicola, isolated from Antarctic soil, secretes an acidic protease into the medium. The secretion of this protease by C. humicola was found to be dependent on the composition of the medium. In YPD or yeast nitrogen base medium containing either amino acid...
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Language: | English |
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American Society for Microbiology
1992
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Online Access: | http://dx.doi.org/10.1128/aem.58.6.1918-1923.1992 https://journals.asm.org/doi/pdf/10.1128/aem.58.6.1918-1923.1992 |
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crasmicro:10.1128/aem.58.6.1918-1923.1992 2024-09-09T19:05:19+00:00 Extracellular protease from the antarctic yeast Candida humicola Ray, M K Devi, K U Kumar, G S Shivaji, S 1992 http://dx.doi.org/10.1128/aem.58.6.1918-1923.1992 https://journals.asm.org/doi/pdf/10.1128/aem.58.6.1918-1923.1992 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 58, issue 6, page 1918-1923 ISSN 0099-2240 1098-5336 journal-article 1992 crasmicro https://doi.org/10.1128/aem.58.6.1918-1923.1992 2024-08-19T04:05:30Z The psychrotrophic, dimorphic yeast Candida humicola, isolated from Antarctic soil, secretes an acidic protease into the medium. The secretion of this protease by C. humicola was found to be dependent on the composition of the medium. In YPD or yeast nitrogen base medium containing either amino acids or ammonium sulfate as the nitrogen source, the activity of the protease in the medium was low (basal level). However, when yeast nitrogen base medium was depleted of amino acids or ammonium sulfate and supplemented with proteins, the activity of the enzyme increased. The secretion of the enzyme was greater during exponential growth at low temperatures than during growth at higher temperatures. The purified protease had a molecular mass of 36,000 Da and was inhibited by pepstatin, iodoacetamide, and sodium dodecyl sulfate. Despite the prevalent cold temperatures in Antarctica, this extracellular protease of the psychrotrophic yeast C. humicola was active at temperatures ranging from 0 to 45 degrees C, with an optimum activity at 37 degrees C. Article in Journal/Newspaper Antarc* Antarctic Antarctica ASM Journals (American Society for Microbiology) Antarctic The Antarctic Applied and Environmental Microbiology 58 6 1918 1923 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
description |
The psychrotrophic, dimorphic yeast Candida humicola, isolated from Antarctic soil, secretes an acidic protease into the medium. The secretion of this protease by C. humicola was found to be dependent on the composition of the medium. In YPD or yeast nitrogen base medium containing either amino acids or ammonium sulfate as the nitrogen source, the activity of the protease in the medium was low (basal level). However, when yeast nitrogen base medium was depleted of amino acids or ammonium sulfate and supplemented with proteins, the activity of the enzyme increased. The secretion of the enzyme was greater during exponential growth at low temperatures than during growth at higher temperatures. The purified protease had a molecular mass of 36,000 Da and was inhibited by pepstatin, iodoacetamide, and sodium dodecyl sulfate. Despite the prevalent cold temperatures in Antarctica, this extracellular protease of the psychrotrophic yeast C. humicola was active at temperatures ranging from 0 to 45 degrees C, with an optimum activity at 37 degrees C. |
format |
Article in Journal/Newspaper |
author |
Ray, M K Devi, K U Kumar, G S Shivaji, S |
spellingShingle |
Ray, M K Devi, K U Kumar, G S Shivaji, S Extracellular protease from the antarctic yeast Candida humicola |
author_facet |
Ray, M K Devi, K U Kumar, G S Shivaji, S |
author_sort |
Ray, M K |
title |
Extracellular protease from the antarctic yeast Candida humicola |
title_short |
Extracellular protease from the antarctic yeast Candida humicola |
title_full |
Extracellular protease from the antarctic yeast Candida humicola |
title_fullStr |
Extracellular protease from the antarctic yeast Candida humicola |
title_full_unstemmed |
Extracellular protease from the antarctic yeast Candida humicola |
title_sort |
extracellular protease from the antarctic yeast candida humicola |
publisher |
American Society for Microbiology |
publishDate |
1992 |
url |
http://dx.doi.org/10.1128/aem.58.6.1918-1923.1992 https://journals.asm.org/doi/pdf/10.1128/aem.58.6.1918-1923.1992 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Applied and Environmental Microbiology volume 58, issue 6, page 1918-1923 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.58.6.1918-1923.1992 |
container_title |
Applied and Environmental Microbiology |
container_volume |
58 |
container_issue |
6 |
container_start_page |
1918 |
op_container_end_page |
1923 |
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1809819322797785088 |