Mineralization of glucose and lignocellulose by four arctic freshwater sediments in response to nutrient enrichment
Microbial biomass and activity were examined in four different arctic sediments: littoral lake sediment and profundal lake sediment from Toolik Lake, Alaska, thaw pond sediment, and eroding river bank peat. The thaw pond sediment had the largest viable microbial biomass, while the profundal sediment...
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1992
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Online Access: | http://dx.doi.org/10.1128/aem.58.5.1554-1563.1992 https://journals.asm.org/doi/pdf/10.1128/aem.58.5.1554-1563.1992 |
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crasmicro:10.1128/aem.58.5.1554-1563.1992 2023-11-05T03:39:30+01:00 Mineralization of glucose and lignocellulose by four arctic freshwater sediments in response to nutrient enrichment McKinley, V L Vestal, J R 1992 http://dx.doi.org/10.1128/aem.58.5.1554-1563.1992 https://journals.asm.org/doi/pdf/10.1128/aem.58.5.1554-1563.1992 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 58, issue 5, page 1554-1563 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 1992 crasmicro https://doi.org/10.1128/aem.58.5.1554-1563.1992 2023-10-09T15:50:40Z Microbial biomass and activity were examined in four different arctic sediments: littoral lake sediment and profundal lake sediment from Toolik Lake, Alaska, thaw pond sediment, and eroding river bank peat. The thaw pond sediment had the largest viable microbial biomass, while the profundal sediment had the smallest. Rates of glucose or acetate incorporation into lipids, glucose mineralization, and lignocellulose mineralization (all normalized per unit of biomass) were highest in the river peat sample, however. The kinetics of glucose mineralization in the profundal sediment were very different from those in the other three samples: although the initial rate of mineralization was five times lower than that in the peat and two times lower than that in the littoral and thaw pond sediments, the maximum amount of 14CO2 evolved from [14C]glucose eventually equaled that in the peat and exceeded that in the littoral and thaw pond sediments by 2.0 and 3.5 times, respectively. Carex aquatilis [14C-cellulose]- and [14C-lignin]lignocellulose mineralization rates in the profundal sediment equaled or exceeded those in the littoral sediment after 16 and 46 days, but the pattern of nutrient limitation differed: the profundal sediment was the only one sampled that exhibited nitrogen limitation, while the other three sediments appeared to be limited primarily by phosphorus. The addition of nitrogen and phosphorus together had no cumulative effects on lignocellulose mineralization. When the rates of mineralization or incorporation of glucose are compared with those of lignocellulose, the results of this study indicate that profundal sediment communities may be better able to utilize the more recalcitrant substrates relative to the labile substrates than microbial communities from sediments rich in detritus and standing macrophytes. Article in Journal/Newspaper Arctic Carex aquatilis Alaska ASM Journals (American Society for Microbiology - via Crossref) Applied and Environmental Microbiology 58 5 1554 1563 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology McKinley, V L Vestal, J R Mineralization of glucose and lignocellulose by four arctic freshwater sediments in response to nutrient enrichment |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
Microbial biomass and activity were examined in four different arctic sediments: littoral lake sediment and profundal lake sediment from Toolik Lake, Alaska, thaw pond sediment, and eroding river bank peat. The thaw pond sediment had the largest viable microbial biomass, while the profundal sediment had the smallest. Rates of glucose or acetate incorporation into lipids, glucose mineralization, and lignocellulose mineralization (all normalized per unit of biomass) were highest in the river peat sample, however. The kinetics of glucose mineralization in the profundal sediment were very different from those in the other three samples: although the initial rate of mineralization was five times lower than that in the peat and two times lower than that in the littoral and thaw pond sediments, the maximum amount of 14CO2 evolved from [14C]glucose eventually equaled that in the peat and exceeded that in the littoral and thaw pond sediments by 2.0 and 3.5 times, respectively. Carex aquatilis [14C-cellulose]- and [14C-lignin]lignocellulose mineralization rates in the profundal sediment equaled or exceeded those in the littoral sediment after 16 and 46 days, but the pattern of nutrient limitation differed: the profundal sediment was the only one sampled that exhibited nitrogen limitation, while the other three sediments appeared to be limited primarily by phosphorus. The addition of nitrogen and phosphorus together had no cumulative effects on lignocellulose mineralization. When the rates of mineralization or incorporation of glucose are compared with those of lignocellulose, the results of this study indicate that profundal sediment communities may be better able to utilize the more recalcitrant substrates relative to the labile substrates than microbial communities from sediments rich in detritus and standing macrophytes. |
format |
Article in Journal/Newspaper |
author |
McKinley, V L Vestal, J R |
author_facet |
McKinley, V L Vestal, J R |
author_sort |
McKinley, V L |
title |
Mineralization of glucose and lignocellulose by four arctic freshwater sediments in response to nutrient enrichment |
title_short |
Mineralization of glucose and lignocellulose by four arctic freshwater sediments in response to nutrient enrichment |
title_full |
Mineralization of glucose and lignocellulose by four arctic freshwater sediments in response to nutrient enrichment |
title_fullStr |
Mineralization of glucose and lignocellulose by four arctic freshwater sediments in response to nutrient enrichment |
title_full_unstemmed |
Mineralization of glucose and lignocellulose by four arctic freshwater sediments in response to nutrient enrichment |
title_sort |
mineralization of glucose and lignocellulose by four arctic freshwater sediments in response to nutrient enrichment |
publisher |
American Society for Microbiology |
publishDate |
1992 |
url |
http://dx.doi.org/10.1128/aem.58.5.1554-1563.1992 https://journals.asm.org/doi/pdf/10.1128/aem.58.5.1554-1563.1992 |
genre |
Arctic Carex aquatilis Alaska |
genre_facet |
Arctic Carex aquatilis Alaska |
op_source |
Applied and Environmental Microbiology volume 58, issue 5, page 1554-1563 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.58.5.1554-1563.1992 |
container_title |
Applied and Environmental Microbiology |
container_volume |
58 |
container_issue |
5 |
container_start_page |
1554 |
op_container_end_page |
1563 |
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1781695341251264512 |