Purification and characterization of purine nucleoside phosphorylase from Proteus vulgaris
Purine nucleoside phosphorylase was isolated and purified from cell extracts of Proteus vulgaris recovered from spoiling cod fish (Gadus morhua). The molecular weight and isoelectric point of the enzyme were 120,000 +/- 2,000 and pH 6.8. The Michaelis constant for inosine as substrate was 3.9 x 10(-...
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American Society for Microbiology
1990
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Online Access: | http://dx.doi.org/10.1128/aem.56.5.1435-1439.1990 https://journals.asm.org/doi/pdf/10.1128/aem.56.5.1435-1439.1990 |
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crasmicro:10.1128/aem.56.5.1435-1439.1990 2024-09-15T18:07:16+00:00 Purification and characterization of purine nucleoside phosphorylase from Proteus vulgaris Surette, M Gill, T MacLean, S 1990 http://dx.doi.org/10.1128/aem.56.5.1435-1439.1990 https://journals.asm.org/doi/pdf/10.1128/aem.56.5.1435-1439.1990 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 56, issue 5, page 1435-1439 ISSN 0099-2240 1098-5336 journal-article 1990 crasmicro https://doi.org/10.1128/aem.56.5.1435-1439.1990 2024-06-24T04:09:50Z Purine nucleoside phosphorylase was isolated and purified from cell extracts of Proteus vulgaris recovered from spoiling cod fish (Gadus morhua). The molecular weight and isoelectric point of the enzyme were 120,000 +/- 2,000 and pH 6.8. The Michaelis constant for inosine as substrate was 3.9 x 10(-5). Guanosine also served as a substrate (Km = 2.9 x 10(-5). However, the enzyme was incapable of phosphorylizing adenosine. Adenosine proved to be useful as a competitive inhibitor and was used as a ligand for affinity chromatography of purine nucleoside phosphorylase following initial purification steps of gel filtration and ion-exchange chromatography. Article in Journal/Newspaper Gadus morhua ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 56 5 1435 1439 |
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Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
Purine nucleoside phosphorylase was isolated and purified from cell extracts of Proteus vulgaris recovered from spoiling cod fish (Gadus morhua). The molecular weight and isoelectric point of the enzyme were 120,000 +/- 2,000 and pH 6.8. The Michaelis constant for inosine as substrate was 3.9 x 10(-5). Guanosine also served as a substrate (Km = 2.9 x 10(-5). However, the enzyme was incapable of phosphorylizing adenosine. Adenosine proved to be useful as a competitive inhibitor and was used as a ligand for affinity chromatography of purine nucleoside phosphorylase following initial purification steps of gel filtration and ion-exchange chromatography. |
format |
Article in Journal/Newspaper |
author |
Surette, M Gill, T MacLean, S |
spellingShingle |
Surette, M Gill, T MacLean, S Purification and characterization of purine nucleoside phosphorylase from Proteus vulgaris |
author_facet |
Surette, M Gill, T MacLean, S |
author_sort |
Surette, M |
title |
Purification and characterization of purine nucleoside phosphorylase from Proteus vulgaris |
title_short |
Purification and characterization of purine nucleoside phosphorylase from Proteus vulgaris |
title_full |
Purification and characterization of purine nucleoside phosphorylase from Proteus vulgaris |
title_fullStr |
Purification and characterization of purine nucleoside phosphorylase from Proteus vulgaris |
title_full_unstemmed |
Purification and characterization of purine nucleoside phosphorylase from Proteus vulgaris |
title_sort |
purification and characterization of purine nucleoside phosphorylase from proteus vulgaris |
publisher |
American Society for Microbiology |
publishDate |
1990 |
url |
http://dx.doi.org/10.1128/aem.56.5.1435-1439.1990 https://journals.asm.org/doi/pdf/10.1128/aem.56.5.1435-1439.1990 |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Applied and Environmental Microbiology volume 56, issue 5, page 1435-1439 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.56.5.1435-1439.1990 |
container_title |
Applied and Environmental Microbiology |
container_volume |
56 |
container_issue |
5 |
container_start_page |
1435 |
op_container_end_page |
1439 |
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1810444650920542208 |